Process for preparing spinosad

A spinosad, preparation technology, applied in the field of spinosad preparation, to achieve the effects of low production cost, high yield and high purity

Inactive Publication Date: 2010-01-20
HUNAN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Especially for Lepidoptera and Thysanoptera pests, it has extremely strong selectivity and insecticidal activity, while its toxicity to non-target organisms is very low, and it is very safe for humans and other mammals. So far, it has not been found to be effective with other insecticides. cross resistance

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Inoculate a preservation tube of the actinomycete Saccharopolyspora spinosa S078-8 strain in a shaker flask with seed culture solution, cultivate it for 2.5 days at 30°C, and insert the seed solution into the seeds tank, when inoculating, the pH of the culture medium in the tank is 7.0; (2) put the medium into the seed tank, sterilize it with high-pressure steam, and after cooling to 30°C, insert the activated shake flask seed solution; (3) Fill the fermenter with culture medium, pass through high-pressure steam to sterilize, and after cooling to 30°C, transfer the seed liquid according to 10% of the feeding volume; (4) Seed tank cultivation: at a temperature of 30°C, a tank pressure of 0.5Kg / cm 2 , aeration rate 1:0.8 (V / V), cultured for 2.5 days under the condition of stirring rate 250rpm; (5) fermenter culture: at a temperature of 31°C, a tank pressure of 0.5Kg / cm 2 , aeration rate 1::1.3 (V / V), under the condition of stirring rate 350rpm, cultivated for 11 days;...

Embodiment 2

[0028] (1) Inoculate a preservation tube of the actinomycete Saccharopolyspora spinosa S078-8 strain into a shaker flask containing seed culture solution, cultivate it for 2 days at 32°C, and insert the seed solution into the seeds tank, during inoculation, the pH of the medium in the tank was 6.0; (2) with the step (2) of Example 1; (3) the fermenter was filled with medium, the sterilization method was the same as the step (2), after sterilization , cooled to 28°C, and transferred to the seed liquid according to 15% of the feeding volume; (4) seed tank cultivation: at a temperature of 28°C, the tank pressure is 0.3Kg / cm 2 , ventilation rate 1:0.7 (V / V), cultured for 3 days under the condition of stirring rate 200rpm; (5) fermenter culture: at a temperature of 28°C, a tank pressure of 0.8Kg / cm 2 , ventilation rate 1: 0.5 (V / V), cultivated 12 days under the condition of agitation rate 200rpm; When checking with a microscope that 70% mycelium was lysed, stop the fermentation, pu...

Embodiment 3

[0033] (1) Inoculate a preservation tube of the actinomycete Saccharopolyspora spinosa S078-8 strain into a shaker flask with seed culture solution, cultivate it for 3 days at 28°C, and insert the seed solution into the seeds tank, when inoculating, the pH of the medium in the tank is 8.0; (2) fill the seed tank with medium, and after sterilization, insert the activated shake flask seed solution; (3) load the medium into the fermenter, sterilize After inoculation, cool to 28 ℃, move into seed solution according to 8% of feeding volume; (4) Seed tank cultivation: at temperature 28 ℃, tank pressure 0.6Kg / cm 2 , ventilation rate 1:0.9 (V / V), cultured for 2 days under the condition of stirring rate 300rpm; (5) fermenter culture: at a temperature of 28°C, a tank pressure of 0.8Kg / cm 2 , aeration rate 1:1.5 (V / V), cultured for 10 days at a stirring rate of 450rpm; during this period, the fermented cells in the fermenter entered the logarithmic growth phase, and the stirring speed an...

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PUM

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Abstract

The invention discloses a process for preparing spinosad, which comprises two parts, namely an actinomyces saccharopolyspora spinosa S078-8 fermentation process and a process for extracting a spinosad crystal product by using fermentation liquor which is obtained by fermenting the actinomyces saccharopolyspora spinosa S078-8 and contains the spinosad. The process has the advantages that firstly, the yield is high, the yield of the spinosad finally achieves more than 2,000 to 4,000 milligrams per liter through HPLC measurement, and in particular, the content of the n-butyl spinosad component with highest insecticidal potency in the fermentation liquor reaches 1,500 to 2,800 milligrams per liter; and secondly, the spinosad crystal product has high purity which reaches 95 to 98 percent.

Description

technical field [0001] The invention relates to a process for preparing spinosyns, in particular to a process for preparing spinosyns by utilizing the actinomycete Saccharopolyspora spinosa. Background technique [0002] Spinosyn (spinosad) is a secondary metabolite produced by the soil actinomycete Saccharopolyspora spinosa, which belongs to macrolide compounds, and its main active ingredient is spinosyn A (accounting for about %-90%) and spinosyn D (about 10%-15%). As a new type of high-efficiency broad-spectrum macrolide insecticidal active substance, spinosyn has been proved to have a wide insecticidal spectrum, including Lepidoptera, Thysanoptera, and Coleoptera. , Diptera (Diptera), Hymenoptera (Hymenoptera), Isoptera (Isoptera) and other pests. Especially for Lepidoptera and Thysanoptera pests, it has extremely strong selectivity and insecticidal activity, while its toxicity to non-target organisms is very low, and it is very safe for humans and other mammals. So fa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/62C12R1/01
Inventor 夏立秋丁学知张友明余子全易勇胡胜标罗玉双杨尉黄璠
Owner HUNAN NORMAL UNIVERSITY
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