High-purity syringin, preparation method and application

A syringin and high-purity technology is applied in the field of preparation of high-purity syringin, can solve the problems of being unsuitable for industrial production and high cost, and achieve the effects of low price, less residue and low cost

Inactive Publication Date: 2010-02-10
苑立超
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] According to the current domestic extraction process, the content of syringin in the Acanthopanax plant is very small, and there is no large amount of syringin extracted for industrial use in practical applications, only a

Method used

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  • High-purity syringin, preparation method and application

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Comparison scheme
Effect test

Embodiment 1

[0032] The preparation method of high-purity syringin comprises: dissolving, macroporous adsorption resin chromatography, decolorization, macroporous adsorption resin chromatography, crystallization, and recrystallization, and is characterized in that:

[0033] a. The dissolution is to dissolve the Acanthopanax ointment with 10 to 30 times of water, then let it stand, and centrifuge;

[0034] b. The macroporous adsorption resin chromatography is to filter the centrate and then go to the macroporous resin chromatography column, first remove impurities with water, then remove impurities with low-concentration C1---C5 lower alcohols, and then use C1---C5 - Desorb the lower alcohol of C5, collect the components containing syringin in sections, and concentrate under reduced pressure;

[0035] c. decolorize the decolorized resin on the concentrated solution, and collect the part containing syringin;

[0036] d. The collected liquid is then subjected to macroporous adsorption resin ...

Embodiment 2

[0039] Take Acanthopanax extractum 200g, add 10 times of water to dissolve, then let it stand, and centrifuge. After the centrate is filtered, the liquid is placed on D101 macroporous adsorption resin. Wash with water until colorless, then remove impurities with 5 and 10% ethanol, and desorb with 20% ethanol. Fractions containing syringin were collected and concentrated under reduced pressure. D900 type decolorizing resin was applied to the concentrated solution, and the part containing syringin was collected. The collected liquid was then applied to D101 macroporous adsorption resin, removed with 10% ethanol, then removed with 15% ethanol, desorbed with 40% ethanol, collected the part with a purity greater than 85%, and evaporated to dryness under reduced pressure. Dissolve with ethanol, crystallize, then dissolve with ethanol, and recrystallize to obtain syringin with a purity of 98.56%.

Embodiment 3

[0041] Take Acanthopanax extractum 300g, add 30 times of water to dissolve, then let it stand, and centrifuge. After the centrifuge is filtered, the liquid is placed on the AB-8 macroporous adsorption resin. First wash with water until colorless, then remove impurities with 5 and 15% ethanol, and desorb with 25% ethanol. Fractions containing syringin were collected and concentrated under reduced pressure. D900 type decolorizing resin was applied to the concentrated solution, and the part containing syringin was collected. The collected solution was then applied to D101 macroporous adsorption resin, removed with 5% ethanol, then removed with 10% ethanol, desorbed with 20% ethanol, collected the fraction with a purity greater than 85%, and evaporated to dryness under reduced pressure. Dissolve with ethanol, crystallize, then dissolve with ethanol, and recrystallize to obtain syringin with a purity of 98.67%.

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Abstract

The invention discloses high-purity syringin, a preparation method and application. The current domestic extraction process has quite high cost and is not suitable for industrialized production. The method comprises dissolution, macroporous absorbent resin chromatography, decolorization, crystallization and recrystallization, wherein a, the dissolution comprises dissolving acanthopanax paste by 10to 30 times of water, then keeping the solution standing and centrifugating the solution; b, the chromatography comprises filtering the centrifugated solution and then filling the centrifugated solution in a macroporous resin chromatography column, removing impurities by water, removing the impurities and desorbing the solution by alcohol with low concentration, collecting components of the syringin by segmenting, and reducing the pressure and concentrating the components; c, the concentrated solution decolorization comprises collecting the part containing the syringin; d, the collected solution re-chromatography comprises removing the impurities and desorbing the solution by lower than 10 percent low-grade alcohol, collecting components of which purities are more than 85 percent by segmenting, and reducing the pressure and evaporating the solution; and e, the solution is crystallized or recrystallized by ethanol of different concentrations to obtain the high-purity syringin. The extracted syringin has low cost, and creates the condition for wide application of medicine produced by the raw materials.

Description

Technical field: [0001] The invention relates to a preparation method and application of high-purity syringin. Background technique: [0002] At present, there is no high-purity syringin bulk drug for the pharmaceutical industry to use in large quantities. Syringin is a substance isolated and extracted from the bark of Oleaceae Syringa vulgaris L., Araliaceae Acanthopanax and other plants. It is the main active ingredient in Chinese medicinal materials such as Acanthopanax. Molecular formula: C 17 h 24 o 9 , molecular weight: 372.36, has a clear chemical structure: [0003] [0004] According to the current domestic extraction process, the content of syringin in the Acanthopanax plant is very small, and there is no large amount of syringin extracted for industrial use in practical applications, only a small amount of it exists in the extract of Acanthopanax as a traditional Chinese medicine Syringin, the high-purity syringin extracted in the prior art, has a very high...

Claims

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Application Information

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IPC IPC(8): C07H15/203A61K31/7034A61P9/00A61P1/16A61P35/00A61P25/28A61P25/20
Inventor 苑立超苑树俐
Owner 苑立超
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