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Preparation method of CdTe-doxorubicin hydrochloride fluorescent probe

A technology of doxorubicin hydrochloride and probe is applied in the directions of biochemical equipment and methods, chemical instruments and methods, luminescent materials, etc., and achieves the effects of low cost, good fluorescence characteristics and simple synthesis method.

Inactive Publication Date: 2010-02-10
NORTHEAST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the current research is mainly focused on biomarkers, and quantum dot probes with dual functions (labeling and treatment) have not been reported yet.

Method used

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  • Preparation method of CdTe-doxorubicin hydrochloride fluorescent probe
  • Preparation method of CdTe-doxorubicin hydrochloride fluorescent probe
  • Preparation method of CdTe-doxorubicin hydrochloride fluorescent probe

Examples

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Effect test

Synthetic example 1

[0020] Take 0.0014g of doxorubicin hydrochloride and dissolve it in 20mL of secondary water, add 6mL to reduce CdCl with NaHTe 2 The pH of the CdTe solution prepared and purified by isopropanol was adjusted to be neutral. Under the protection of nitrogen, the reaction system was heated to 40° C., condensed and refluxed for 20 minutes to obtain the CdTe-doxorubicin hydrochloride probe.

Synthetic example 2

[0022] Dissolve 0.0008 g of doxorubicin hydrochloride in 30 mL of secondary water, add 9 mL of the CdTe solution prepared according to the method of Example 1 above, and adjust the pH to be neutral. Under the protection of nitrogen, the reaction system was heated to 80° C., condensed and refluxed for 40 minutes to obtain the CdTe-doxorubicin hydrochloride probe.

Synthetic example 3

[0024] Dissolve 0.0007 g of doxorubicin hydrochloride in 24 mL of secondary water, add 6 mL of the CdTe solution prepared according to the method of Example 1 above, and adjust the pH to be neutral. Under the protection of nitrogen, the reaction system was heated to 40° C., condensed and refluxed for 20 minutes to obtain the CdTe-doxorubicin hydrochloride probe.

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Abstract

The invention belongs to the technical fields of semiconductors and biological tags, particularly relates to a preparation method of a CdTe-doxorubicin hydrochloride fluorescent probe, which comprisesthe following steps: using surface finishing technology, mixing doxorubicin hydrochloride and purified orange CdTe solution to regulate the pH value of the system; under the protection of nitrogen, heating and reflux-condensing to prepare the CdTe-doxorubicin hydrochloride fluorescent probe. The fluorescent probe can produce strong fluorescence under the irradiation of an ultraviolet lamp; and through spectroscopic analysis, the CdTe-doxorubicin hydrochloride fluorescent probe has favorable fluorescence properties. Strong interactions exist between the CdTe-doxorubicin hydrochloride fluorescent probe and polypeptides, and the CdTe-doxorubicin hydrochloride fluorescent probe is successfully used for tagging hepatoma carcinoma cells. The method has the advantages of simple method for synthesizing materials, easy control on components, low cost, favorable fluorescence properties of the obtained quantum dot probe, and the like.

Description

technical field [0001] The invention belongs to the technical field of semiconductors and biomarkers, and in particular relates to a preparation method of a fluorescent probe. Background technique [0002] Due to the particularity and complexity of biological systems, in order to detect more biologically active substances, there are high requirements for laser-excited bioluminescent probes. At present, the most commonly used fluorescent probes are organic dyes, but their excitation spectra are narrow, so it is difficult to excite multiple components at the same time; and their fluorescence emission spectra are wide, and the distribution is asymmetric, so it is necessary to simultaneously Detection of multiple components is difficult. The most serious defect of organic dyes is poor photochemical stability. Photobleaching and photolysis make it impossible for each dye probe to emit too many fluorescent photons on average, and photolysis products often have a killing effect on...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/88C09K11/06C12Q1/02
Inventor 尚庆坤冯雪娇刘俊余单桂晔张维静刘红剑
Owner NORTHEAST NORMAL UNIVERSITY