Method for integrated application of bulrush biotic substance

An application method and biomass technology are applied in the comprehensive application field of reed biomass to achieve the effects of easy absorption and utilization, high feeding value and beautifying living environment.

Inactive Publication Date: 2010-02-17
YANCHENG INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But at present, there is no enterprise in the world that uses cellulosic raw materials to produce fuel ethanol on an industrial scale

Method used

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  • Method for integrated application of bulrush biotic substance
  • Method for integrated application of bulrush biotic substance

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Remove the rotten and dead leaves, wash them, dry them naturally, crush them with a grinder, and sieve them through a 60-mesh sieve. Using supercritical CO 2 The total flavonoids in reed leaves were extracted by extraction technology, and the four factors of extraction temperature, time, pressure and entrainer dosage were studied, and the total flavonoids in the extract were quantitatively analyzed by ultraviolet spectrophotometry. Supercritical CO 2The optimal process conditions for extracting total flavonoids are: temperature 65℃, time 1.5h, pressure 30MPa, entrainer dosage 4.5ml / g. The extraction rate of flavonoids is 70.48%.

Embodiment 2

[0041] Mix well-growing colonies on the medium with a small amount of distilled water with a coating stick, then pour the bacterial liquid into the sterilized and cooled liquid medium, and put it in a shaker (at a temperature of 37°C and a rotation speed of 100r / min) for cultivation 24h to measure its absorbance. After adding 10ml reed extract in each bacterium suspension, shaking table culture 24h measures the absorbance of each bacterium suspension respectively again, as shown in table 1; Bacteriostasis %=(absorbance of original bacterium liquid-adding the absorbance of bacterium liquid after extract) Absorbance) / absorbance of the original bacterial solution × 100%, and the bacteriostatic rate is shown in Table 2.

[0042] Table 1 The comparison of the absorbance of each bacterial strain before and after adding the extract

[0043]

[0044] Table 2 The inhibition rate of reed extract to various strains

[0045] bacteria

[0046] Therefore, it can be preliminar...

Embodiment 3

[0048] Determination of the antioxidative activity of reed extract to oil: Weigh 4 parts of 30g oil (Luhua peanut oil) and place it in a beaker, 1 part is the blank control group (without extracting solution), and 3 parts are measured according to 0.05% of the oil weight, 0.05% of the oil weight, 0.10%, 0.50% added to the extract. Mix evenly, place in a constant temperature oven at (60±0.5)°C, cover each beaker with a watch glass, stir once every 12 hours, and exchange its position in the oven. Samples were taken every few days to measure the peroxide value (POV) of peanut oil, and this was used to represent the oxidation rate of peanut oil, and then to calculate the antioxidant activity of the extract. Accurately weigh 3g of the mixed sample (filter if necessary), put it into an iodine measuring bottle, add 30mL of chloroform-glacial acetic acid mixture, dissolve the sample, add 0.5mL of saturated potassium iodide solution, immediately stopper and shake for 1min, and keep it ...

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Abstract

The invention relates to a technical method for the integrated application of a bulrush biotic substance. The method for the integrated application of the bulrush biotic substance comprises the following steps: firstly, adopting supercritical extraction to extract and separate a biological active substance in the stem and leaves of bulrush and obtaining an optimal technological condition; concentrating an extracting solution by depressurization and forming a concentrated solution as an application method of an antioxidant and a bacteriostatic agent; establishing a foundation for the research of the bulrush as a purely natural green medicine source; carrying out diastatic fermentation after carrying out corresponding preprocessing on the residues of the stem and leaves of the bulrush rich in cellulose biotic substances after extracting the biological active substance, and forming a production method of biological fuel of ethanol; taking the residues of the bulrush which is rich in single cell protein (SCP) and is easy to absorb after being fermented by microzymes as the feed of milch cows and obtaining the feeding value of the bulrush; and forming an integrated application system ofthe biotic substance of the bulrush and providing a foundation for the integrated biologic utilization of the bulrush.

Description

technical field [0001] The invention relates to a technical method for extracting active components of reed biomass, producing cellulose ethanol, and producing straw protein feed. Background technique [0002] Reed (Phragmitescommunis.), a perennial plant of the grass family Phragmites genus, has strong stress resistance and survival competitiveness. It is widely distributed all over the country. Under natural conditions, reeds are rarely attacked by diseases and insect pests. Therefore, it is speculated that it contains and releases highly active substances that can control the pathogens and pests that invade it, and can inhibit the survival of other plants. Competition for space etc. The rapid growth of reed has become a major problem restricting the development of corresponding areas, and it cannot be turned into industrial raw materials with high added value and high-tech content. In addition to being used as weaving and papermaking materials, most of them are burned d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B5/00A23K1/16C12P7/10A01N65/44A01P1/00C12R1/645A23K20/147
CPCY02E50/16Y02E50/10Y02P20/54
Inventor 余晓红邵荣董锐刘必前许伟
Owner YANCHENG INST OF TECH
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