Paraffin wax slicing method of ocean shellfish oocyte
A paraffin section and egg cell technology, which is applied to measurement devices, material analysis by optical means, instruments, etc., can solve the problems of small eggs and have not yet been seen, achieve clear egg sections, facilitate popularization and application, and overcome inconvenience in operation.
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Embodiment 1
[0036] 1. Sample fixation: Take meretrix meretrix from Shandong population, and conduct research on oxytocin and embryonic development at the Qingjiang Research Base of the Provincial Marine Aquaculture Research Institute in Wenzhou, Zhejiang. Take clam eggs of different development stages in 5mL centrifuge tubes, take samples every 5 minutes for 0-60 minutes after fertilization, and take samples every 10 minutes for 60-120 minutes, slowly add 2-3 mL of cold Bouin's fixative solution, and fix for 30 minutes. After the sample settles, replace the secondary fixative; fix with Bouin's solution for 5 hours, transfer to 70% alcohol, and store in a refrigerator at 4°C.
[0037] 2. Egg cell pretreatment: add 2 drops of 1% eosin dye solution to the centrifuge tube containing the egg cells, and stain for 1-2 minutes to make the surface of the egg cells dyed pink; take a square weighing paper and use a fine needle Puncture some small holes in the paper, then fold the paper, and wrap the...
Embodiment 2
[0044] The wide-shelled whole sea bamboo shoots are taken from the sea area of Dongtou County, Wenzhou. After fertilization, fertilized eggs of different development stages are fixed. Since the diameter of the wide-shelled whole sea bamboo shoot eggs is only 40-50 μm, it is easy to lose when changing the fixative, so the amount of fixed eggs should be larger, and the centrifuge should be centrifuged at low speed to speed up the sedimentation.
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