Compound bactericidal composition containing boscalid

A technology of boscalid and its composition, which is applied in the field of compound bactericidal composition containing boscalid, can solve the problems of high cost and resistance, reduce production and use costs, reduce single selection pressure, The effect of reducing the dosage of pesticides in the field

Inactive Publication Date: 2010-02-24
SHENZHEN NOPOSION AGROCHEM
0 Cites 13 Cited by

AI-Extracted Technical Summary

Problems solved by technology

Boscalid is a new and highly effective agent for the control of botrytis cinerea a...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Abstract

The invention relates to a bactericidal composition, the effective ingredients of which are boscalid (A) and any one (B) of prochloraz, cyprodinil and fluazinum, wherein, the mass ratio of A to B is 50:1-1:50. The A and B have obvious synergized action after being compounded, and the compound bactericidal composition can be used for preventing and treating diseases, especially fungal diseases of crops, such as fruit trees, flowers, cotton, wheat, paddy, rape, vegetables and the like,.

Application Domain

Technology Topic

Image

  • Compound bactericidal composition containing boscalid
  • Compound bactericidal composition containing boscalid

Examples

  • Experimental program(9)

Example Embodiment

[0022] Bioassay Example 1: Toxicity determination of a compound of boscalid (A) and prochloraz, cyprodinil, and fluazinam (B) against cucumber gray mold
[0023] Test object: Cucumber Botrytis cinerea Pers.
[0024] The potted method was used in this experiment. Cucumber gray mold susceptible to sowing varieties, grown in pots until 2-4 true leaves, numbered for later use. On the basis of the preliminary test, set 5-7 series of mass concentrations according to the activity of the drug. For each treatment of 4 pots, spray 10ml of the medicinal solution evenly on the leaves until they are completely wet, and wait for the medicinal solution to dry naturally for later use. And the treatment containing only solvent and surfactant but no active ingredient was set as blank control. After 24 hours of chemical treatment, a crop sprayer was used to evenly spray and inoculate the conidia suspension of Botrytis cinerea on cucumber leaves. The preparation process of the conidia suspension of cinerea cinerea was as follows: the pathogenic bacteria used in the test were cultivated on a suitable medium until a large number of spores were produced, then the spores were washed with sterile water, filtered with double-layer gauze, and prepared into 1 ×10 5 spores/mL spore suspension (add a little surfactant to facilitate attachment). After inoculation, move to a humidity chamber (more than 95% relative humidity, temperature 20-22° C.) and cultivate in the dark for 24 hours, then cultivate for 7 days at 20-25° C. and relative humidity 80%-90%. The lesion area on each cucumber leaf was counted, and the disease index and control effect were calculated. 16 leaves were investigated for each treatment, and the grading method was as follows:
[0025] Cucumber botrytis grading standard
[0026] Grade 0: no disease spots on the leaves;
[0027] Grade 1: Lesion area accounts for less than 5% of the entire leaf area;
[0028] Grade 3: The lesion area accounts for 6-15% of the entire leaf area;
[0029] Grade 5: Lesions account for 16-25% of the entire leaf area;
[0030] Grade 7: The lesion area accounts for 26-50% of the entire leaf area;
[0031] Grade 9: The lesion area accounts for more than 50% of the entire leaf area.
[0032] Drug efficacy calculation method:
[0033]
[0034] The control effect is converted into a probability value (y), and the concentration of the drug solution (μg/ml) is converted into a logarithmic value (x), and the toxicity equation and the inhibitory intermediate concentration EC are calculated by the least square method 50 According to Sun Yunpei's method, the toxicity index and co-toxicity coefficient (CTC) of the drug were calculated.
[0035] Measured Toxicity Index (ATI) = (standard drug EC 50 /Test agent EC 50 )×100
[0036] Theoretical toxicity index (TTI) = Toxicity index of agent A × percentage of A in the mixture + toxicity index of agent B × percentage of B in the mixture
[0037] Co-toxicity coefficient (CTC) = [mixture measured toxicity index (ATI) / mixture theoretical toxicity index (TTI)] × 100
[0038] When CTC≤80, the composition exhibits antagonistic effect; when 80
[0039] See Table 1 to Table 3 for toxicity test results
[0040] Table 1 Pot test results of boscalid and prochloraz series ratios on cucumber botrytis
[0041] deal with
[0042] The test results showed that when the compounding ratio of boscalid and prochloraz was between 50:1 and 1:50, the co-toxicity coefficient was above 136.3, showing a synergistic effect; the compounding ratio of 20 :1-1:20, the co-toxicity coefficients are all higher than 177.8, and the synergistic effect is more obvious.
[0043] Table 2 Pot test results of boscalid and cyprodinil series ratios on cucumber botrytis
[0044] deal with
[0045] The test results show that when the compounding ratio of boscalid and cyprodinil is between 50:1-1:50, the co-toxicity coefficient is above 136.2, which has a synergistic effect; Between 20:1-1:20, the co-toxicity coefficients are all higher than 187.1, and the synergistic effect is more obvious.
[0046] Table 3 Pot test results of boscalid and fluazinam series ratios on cucumber botrytis
[0047] deal with
[0048] Boscalid 1: fluazinam 1
[0049] The test results show that when the combination of boscalid and fluazinam controls cucumber gray mold, when the ratio is between 50:1-1:50, the co-toxicity coefficient is above 133.5, which has a synergistic effect; Between 20:1-1:20, the co-toxicity coefficients are all higher than 178.6, and the synergistic effect is more obvious.

Example Embodiment

[0050] Bioassay Example 2: Indoor Toxicity Test of Cyazofamid (A) and Prochloraz, Cyprodinil, and Fluazinam (B) against Cucumber Powdery Mildew
[0051] Test object: Cucumber powdery mildew (Erysiphe cichoracearum DC.)
[0052] The potted method was used in this experiment. Sowing cucumber powdery mildew varieties, the potted plant grows to 2-4 true leaf stage, and the number is reserved. On the basis of the preliminary test, set 5-7 series of mass concentrations according to the activity of the drug. Spray the liquid medicine evenly on the leaf surface until it is completely moistened, and wait for the liquid medicine to dry naturally for later use. Four pots were treated for each treatment, and the treatment containing only solvent and surfactant but no active ingredient was set as a blank control. 24 hours after the application, the powdery mildew spore suspension was sprayed and inoculated. The preparation process of the spore suspension is as follows: use pure water with a small amount of Tween-80 to absorb the fresh spores on the cucumber leaves covered with powdery mildew fungus, and make the spore concentration 1×10 5 individual/mL spore suspension. After culturing in the greenhouse for 7 days, the lesion area on the leaves of each cucumber seedling was counted, and the disease index and control effect were calculated.
[0053] Cucumber Powdery Mildew Grading Standard
[0054] Level 0: no disease;
[0055] Level 1: The spore pile area accounts for less than 5% of the entire leaf area;
[0056] Level 3: The spore pile area accounts for 6%-10% of the entire leaf area;
[0057] Grade 5: The spore pile area accounts for 11%-20% of the entire leaf area;
[0058] Grade 7: The spore pile area accounts for 21%-50% of the entire leaf area;
[0059] Grade 9: The spore pile area accounts for more than 50% of the entire leaf area.
[0060] Drug efficacy calculation method:
[0061]
[0062] The control effect was converted into a probability value (y), the concentration of the drug solution (μg/ml) was converted into a logarithmic value (x), and the toxicity equation and the inhibitory intermediate concentration EC were calculated by the least square method 50 , The calculation and analysis methods of toxicity index and co-toxicity coefficient are the same as above.
[0063] See Table 4 to Table 6 for toxicity assay results
[0064] Table 4 Pot test results of the series ratio of boscalid and cyprodinil to cucumber powdery mildew
[0065] deal with
[0066] The test results show that when the combination of boscalid and cyprodinil controls cucumber powdery mildew, when the ratio is between 50:1-1:50, the co-toxicity coefficient is above 137.1, which has a synergistic effect; Between 20:1-1:20, the co-toxicity coefficients are all higher than 172.7, and the synergistic effect is more obvious.
[0067] Table 5 Determination results of pot experiments on cucumber powdery mildew with series ratios of boscalid and fluazinam
[0068] deal with
[0069] The test results show that when the combination of boscalid and fluazinam controls cucumber powdery mildew, when the ratio is between 50:1-1:50, the co-toxicity coefficient is above 132.5, which has a synergistic effect; when the ratio is 20 :1-1:20, the co-toxicity coefficients are all higher than 174.6, and the synergistic effect is more obvious.
[0070] Table 6 Determination results of pot experiments on cucumber powdery mildew with series ratios of boscalid and prochloraz
[0071] deal with
[0072] Boscalid 5: Prochloraz 1
[0073] The test results show that the combination of boscalid and prochloraz can prevent and control cucumber powdery mildew. When the ratio is between 50:1-1:50, the co-toxicity coefficient is above 134.6, which has a synergistic effect; when the ratio is 20 :1-1:20, the co-toxicity coefficients are all higher than 179.3, and the synergistic effect is more obvious.
[0074] The above determination results show that when the ratio of boscalid (A) to one of prochloraz, cyprodinil and fluazinam (B) is between 50:1-1:50, there is a synergistic effect , between 20:1-1:20, the co-toxicity coefficient is above 172.7, and the synergistic effect is more obvious.
[0075] The fungicidal composition of the present invention can be prepared into wettable powders, suspension concentrates, water-dispersible granules, emulsifiable concentrates, water-emulsions, and microemulsions suitable for agricultural use by known methods. Illustrate below with specific embodiment, percentage is mass percent in formula. The active ingredient in this application document refers to one of boscalid (A) and prochloraz, cyprodinil, and fluazinam (B), and will not be described in detail below.

Example Embodiment

[0076] Example 1: 10.2% boscalid prochloraz EC
[0077] Boscalid 10%
[0078] Prochloraz 0.2%
[0079] N-methylpyrrolidone (solvent) 10%
[0080] DMF (solvent) 10%
[0081] Nongru 1601# (emulsifier) ​​5%
[0082] Nongru 500# (emulsifier) ​​5%
[0083] Xylene (solvent) was made up to 100%.
[0084] Active ingredients, solvents, and emulsifiers are sequentially added into the mixing kettle according to the ratio of the formula, and stirred evenly to prepare 10.2% boscalid-prochloraz EC.
[0085] This embodiment is applied to the control of strawberry gray mold. Dilute and spray 10.2% boscalid prochloraz EC 500 times (the effective concentration of boscalid is 200 μg/ml, and the effective concentration of prochloraz is 4 μg/ml) with water, and the prevention and control of 7 days and 15 days after treatment The effects were 93.8% and 92.2%, respectively. 25% boscalid suspending agent was used at 1000 times (the effective concentration of boscalid was 250 μg/ml) and 25% prochloraz EC was used at 1000 times (the effective concentration was 250 μg/ml), and used in the same way. The control effects of days and 15 days were 86.1%, 85.3% and 87.4%, 80.1%, respectively. The combination of boscalid and prochloraz has obvious synergistic effect, and the control effect on strawberry botrytis is obviously better than that of single agent.
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

no PUM

Description & Claims & Application Information

We can also present the details of the Description, Claims and Application information to help users get a comprehensive understanding of the technical details of the patent, such as background art, summary of invention, brief description of drawings, description of embodiments, and other original content. On the other hand, users can also determine the specific scope of protection of the technology through the list of claims; as well as understand the changes in the life cycle of the technology with the presentation of the patent timeline. Login to view more.
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Similar technology patents

Classification and recommendation of technical efficacy words

  • Improve the effect of prevention and control
  • Reduce field use

Bactericidal composition for preventing and controlling potato black scurf

ActiveCN104996429AReduce field useBiocideFungicidesToxicitySuspending Agents
Owner:SHANDONG AGRICULTURAL UNIVERSITY

Insecticidal sterilizing sustained-release granule containing clothianidin and tebuconazole and application thereof

InactiveCN103340209AReduce field useControl the occurrence of pests and diseasesBiocideFungicidesControl periodDisease damage
Owner:PLANT PROTECTION & QUALITY & SAFETY OF AGRI PRODS INST ANHUI ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products