Albumin-adriamycin nano preparation, preparing method and application thereof
A nano preparation, albumin technology, applied in the field of medicine
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Embodiment 1
[0016] Embodiment 1. Dissolving bovine serum albumin (BSA) and dextran (62kDa) with deionized water, its molar feed ratio is 2: 1, after the solution is mixed evenly, its pH value is adjusted to 7.1, then the solution is frozen dry. The freeze-dried solid powder was weighed, then placed in a beaker and placed in a closed container with KBr saturated solution (the relative humidity in the container was 79%), and the albumin- Dextran covalent complex. Dissolve the albumin-dextran covalent complex with deionized water, prepare a solution with an albumin concentration of 1 mg / mL, and mix it with an acetic acid solution of chitosan (molecular weight 50 kDa), so that the mass of chitosan and albumin The ratio was 1:10, and the final concentration of albumin was 0.5 mg / mL. After the pH value of the mixed solution is adjusted, the chitosan / dextran-albumin nanoparticles can be prepared by heating at 80° C. for 1 hour.
[0017] The particle size and polydispersity coefficient of the ...
Embodiment 2
[0020] Embodiment 2. will carry out the bovine serum albumin-dextran covalent complex that obtains after 24 hours Maillard reaction is dissolved with deionized water, add the chitosan (molecular weight 50kDa) acetic acid solution of different ratios, then adjust solution pH To 5.6, prepare chitosan / dextran-albumin nanoparticles by heating at 80°C for 1 hour. The results in Table 2 show that chitosan / dextran-albumin nanoparticles can be prepared within a certain range of mass ratios of chitosan and albumin.
[0021] Table 2. Chitosan / dextran-albumin nanoparticles prepared under different mass ratios of chitosan and albumin.
[0022] Mass ratio of chitosan to albumin particle size polydispersity coefficient 0 187 0.13 0.1 127 0.14 0.2 131 0.22 0.3 131 0.24 0.4 139 0.26 1.0 377 0.37 1.2 410 0.27 1.4 429 0.40 1.6 459 0.42 1.8 510 0.38 2.0 4...
Embodiment 3
[0023] Embodiment 3. will carry out the bovine serum albumin-dextran covalent complex obtained after 24 hours Maillard reaction with deionized water dissolving, add chitosan (molecular weight 50~190kDa) acetic acid solution, chitosan and white The mass ratio of the protein was 1:10, then the pH of the solution was adjusted to 5.6, and the chitosan / dextran-albumin nanoparticles were prepared by heating at 80° C. for 1 hour.
[0024]Nanoparticles prepared by this method are very stable, and the particle size hardly changes after a long time in pH 7.4PBS solution, indicating that dextran exists in the outer shell of nanoparticles, which prevents chitosan from being cross-linked due to hydrogen bonds. and the aggregation that occurred. ζ-potential analysis showed that (attached figure 2 ), the ζ-potential of the prepared nanoparticles was similar to that of chitosan alone at low pH, indicating that chitosan and dextran together constitute the shell of nanoparticles, while the co...
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