Method for preparing degradant of Venetian acinetobacter tetradecane by semisolid fermentation method based on using bagasse as immobilized carrier

Abstract

The invention discloses a method for preparing degradant of Venetian acinetobacter tetradecane by a semisolid fermentation method based on using bagasse as immobilized carrier. In the invention, expanding cultivation is carried out on the Venetian acinetobacter by preparing conventional inorganic salt culture medium, and bagasse is utilized as immobilized carrier to prepare the degradant capable of efficiently degrading tetradecane in waste water and liquid waste. The process of the method is as follows: carrying out grinding, sieving and high pressure sterilization on bagasse after bagasse iscleaned and dried; introducing Venetian acinetobacter and bagasse powder in the inorganic salt culture medium, stirring and blending, and then carrying out liquid swing bed shaking primary culture; adding bagasse powder after 6 to 12h primary culture, mixing evenly and culturing again; and filling a double-layer gauze mesh bag with the culture, tying and then suspending the mesh bag in waste water required to be degraded. By adopting the method, the degradation ratio of 50.0mg / L tetradecane can be up to 89%, the degradation ratio to tetradecane with the same concentration can be kept at 96-98%, and has a wonderful effect on degrading alkane in diesel oil.

Description

technical field [0001] The invention relates to waste water treatment technology, in particular to a method for preparing a tetradecane degrading agent in waste water degraded by Acinetobacter venetianus by a semi-solid fermentation method based on bagasse as an immobilized carrier. technical background [0002] With the rapid development of social economy and the continuous improvement of people's living standards, the discharge of production and domestic sewage has increased sharply, and global water pollution has become increasingly serious, threatening the sustainable development of social economy and even human beings themselves. Among the various methods of wastewater, the biological method is favored because of its advantages of low cost, less land occupation, and no secondary pollution. Although the traditional biological method has unique advantages compared with other treatment methods, in the traditional wastewater treatment process, microorganisms generally grow ...

AI Technical Summary

Problems solved by technology

There is no unified classification standard for the immobilization method of microorganisms at home and abroad.