Method for preparing degradant of Venetian acinetobacter tetradecane by semisolid fermentation method based on using bagasse as immobilized carrier
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A technology of immobilized carrier and semi-solid fermentation, applied in the field of preparation of tetradecane degrading agent
Inactive Publication Date: 2010-03-10
FUJIAN NORMAL UNIV
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Problems solved by technology
There is no unified classification standard for the immobilization method of microorganisms at home and abroad.
Method used
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Embodiment 1
[0026] 1. Take the components in the subordinate formula respectively, and prepare an inorganic salt medium with a pH value of 7.0 according to the conventional preparation method:
[0027] K 2 HPO 4 10g
[0028] K H 2 PO 4 10g
[0029] NH 4 NO 3 10g
[0030] MgSO 4 3g
[0031] CaCl 2 0.3g
[0032] FeSO 4 0.05g
[0033] ZnSO 4 0.02g
[0034] MnSO 4 0.002g
[0035] Distilled water 5000mL
[0036] The culture medium prepared according to the above formula was sterilized by high pressure and used for later use.
[0037] 2. Pretreatment of bagasse
[0038] Wash 1 kg of bagasse with tap water, dry it at 60° C., pulverize it with a pulverizer, sieve it, and take bagasse powder with a mesh number of 40 to 60 mesh, and use a DSX-28013 stainless steel portable sterilizer ( Shanghai Shenyi Medical Instrument Factory), after being autoclaved at 121°C for use.
[0039] 3. Preparation of degradation a...
Embodiment 2
[0044] Steps 1 and 2 are the same as in Example 1
[0045] 3. Preparation of degradation agent
[0046]Take 1000ml of the pre-prepared medium, place it in the SPX-250B-Z biochemical incubator (Shanghai Boxun Industrial Co., Ltd. Medical Equipment Factory), insert 50ml of Acinetobacter venetianus bacteria solution (Acinetobacter venetianus), and the volume of the medium 200g of 40-60 mesh bagasse powder was stirred and mixed evenly, the temperature was controlled at 35°C, and the shaking culture was carried out on a liquid shaking table, and the shaking speed of the shaking table was 150r / min. After cultivating for 8 hours, keep the temperature constant, then add 150g of 40-60 mesh bagasse, mix well, form a semi-dry and wet state for static cultivation, and cultivate for 8 hours to make a semi-dry bagasse-based immobilized carrier. Preparation of tetradecane-degrading agent of Acinetobacter veniceus by solid fermentation method.
[0047] 4. Bagging and hanging
[0048] The p...
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Abstract
The invention discloses a method for preparing degradant of Venetian acinetobacter tetradecane by a semisolid fermentation method based on using bagasse as immobilized carrier. In the invention, expanding cultivation is carried out on the Venetian acinetobacter by preparing conventional inorganic salt culture medium, and bagasse is utilized as immobilized carrier to prepare the degradant capable of efficiently degrading tetradecane in waste water and liquid waste. The process of the method is as follows: carrying out grinding, sieving and high pressure sterilization on bagasse after bagasse iscleaned and dried; introducing Venetian acinetobacter and bagasse powder in the inorganic salt culture medium, stirring and blending, and then carrying out liquid swing bed shaking primary culture; adding bagasse powder after 6 to 12h primary culture, mixing evenly and culturing again; and filling a double-layer gauze mesh bag with the culture, tying and then suspending the mesh bag in waste water required to be degraded. By adopting the method, the degradation ratio of 50.0mg / L tetradecane can be up to 89%, the degradation ratio to tetradecane with the same concentration can be kept at 96-98%, and has a wonderful effect on degrading alkane in diesel oil.
Description
technical field [0001] The invention relates to waste water treatment technology, in particular to a method for preparing a tetradecane degrading agent in waste water degraded by Acinetobacter venetianus by a semi-solid fermentation method based on bagasse as an immobilized carrier. technical background [0002] With the rapid development of social economy and the continuous improvement of people's living standards, the discharge of production and domestic sewage has increased sharply, and global water pollution has become increasingly serious, threatening the sustainable development of social economy and even human beings themselves. Among the various methods of wastewater, the biological method is favored because of its advantages of low cost, less land occupation, and no secondary pollution. Although the traditional biological method has unique advantages compared with other treatment methods, in the traditional wastewater treatment process, microorganisms generally grow ...
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