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Ahas mutants

A double mutation, valine technology, applied in the direction of recombinant DNA technology, introduction of foreign genetic material using a vector, lyase, etc., can solve the problem of imidazolinone specific resistance and other problems that are not described

Active Publication Date: 2010-03-10
BASF PLANT SCI GMBH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The mutations described in those documents encode cross-resistance or sulfonylurea-specific resistance to imidazolinones and sulfonylureas, but imidazolinone-specific resistance is not described

Method used

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[0149] 1. Dilute the product for foliar application with water. For seed treatment purposes, such products may be applied to the seed with or without dilution.

[0150] A) Water-soluble concentrate (SL, LS)

[0151] 10 parts by weight of the AHAS-inhibiting herbicide is dissolved in 90 parts by weight of water or a water-soluble solvent. Alternatively, wetting agents or other auxiliaries are added. After dilution with water, the AHAS-inhibiting herbicide was dissolved, thus obtaining a formulation containing 10% (w / w) of the AHAS-inhibiting herbicide.

[0152] B) Dispersible Concentrate (DC)

[0153] 20 parts by weight of an AHAS-inhibiting herbicide was dissolved in 70 parts by weight of cyclohexanone, and 10 parts by weight of a dispersant (for example, polyvinylpyrrolidone) was added. Dilution with water produces a dispersant, thus obtaining a formulation containing 20% ​​(w / w) of the AHAS-inhibiting herbicide.

[0154] C) Emulsifiable Concentrate (EC)

[0155] 15 par...

Embodiment 1

[0188] Vectors Containing Arabidopsis AHASL Mutant Genes

[0189] A complete XbaI fragment of Arabidopsis genomic DNA containing the complete AHAS large subunit gene with some additional DNA included in the 5' and an XbaI site at the 3' end. Bases 2484 to 4496 of SEQ ID NO: 34 include the coding sequence of the serine to threonine mutant allele at position 653 of the Arabidopsis AHAS large subunit gene, including the stop codon shown in SEQ ID NO: 30 son. Smaller genome of the serine to threonine mutant allele at position 653 of the Arabidopsis AHAS large subunit gene shown in SEQ ID NO: 33 included in bases 2484 to 5717 of SEQ ID NO: 34 The fragment includes the coding sequence and the 3' end up to and including the 3' end XbaI site, where the first two bases of the NcoI site present at the start codon of AtAHASL were removed for clarity.

[0190] The DNA fragment SEQ ID NO: 33 encoding the full-length Arabidopsis AHASL single mutant S653N and the 3' untranslated region wa...

Embodiment 2

[0201] Embodiment 2: the carrier of maize AHASL mutant gene

[0202] The maize AHASL2 gene (SEQ ID NO:29) was cloned into the bacterial expression vector pTrcHisA (Invitrogen Corporation, Carlsbad, CA), fused to the vector tag and a translation initiation site starting at base 160 of SEQ ID NO:29. Using ZE1 as the base vector, mutagenesis and subcloning methods were used to generate vectors ZE2, ZE5, ZE6 and ZE7. A subcloning approach was used to generate ZE3 from ZE1, which is the maize AHASL2 gene fused to the vector tag and translation initiation site of pTrcHis A, starting at base 121 of SEQ ID NO:29. Since no functional differences between ZE1 or ZE3 were shown in E. coli, standard mutagenesis and subcloning methods were used to generate vectors ZE4 and ZE8 using ZE3 as the base vector.

[0203] A plant transformation vector with an expression cassette was prepared using standard methods and named ZP1 ( Figure 7 ), wherein the expression cassette comprises a maize ubiq...

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Abstract

The invention provides nucleic acids encoding mutants of the acetohydroxyacid synthase (AHAS) large subunit comprising at least two mutations, for example double and triple mutants, which are useful for producing transgenic or non-transgenic plants with improved levels of tolerance to AHAS-inhibiting herbicides. The invention also provides expression vectors, cells, plants comprising the polynucleotides encoding the AHAS large subunit double and triple mutants, plants comprising two or more AHAS large subunit single mutant polypeptides, and methods for making and using the same.

Description

field of invention [0001] The present invention relates generally to compositions and methods for increasing the tolerance of plants to acetohydroxyacid synthase-inhibiting herbicides. Background of the invention [0002] Acetohydroxyacid synthase (AHAS; EC 4.1.3.18, also known as acetolactate synthase or ALS) is the first enzyme that catalyzes the biochemical synthesis of the branched-chain amino acids valine, leucine and isoleucine ( Singh (1999) "Biosynthesis of valine, leucine and isoleucine," in Plant Amino Acids, edited by Singh B.K., Marcel Dekker Inc. New York, New York, pp. pp. 227-247). AHAS is the site of action of four structurally distinct herbicide families including the sulfonylureas (Tan et al. (2005) Pest Manag. Sci. 61:246-57; Mallory-Smith and Retzinger (2003 ) Weed Technology 17:620-626; LaRossa and Falco (1984) Trends Biotechnol.2:158-161), imidazolinones (Shaver et al. (1984), Plant Physiol.76:545-546), triazolo Pyrimidines (Subramanian and Gerwick (...

Claims

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Application Information

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IPC IPC(8): A01H5/00A01H5/10C12N15/82C12N9/88
CPCC12N9/88C12N15/8274C12N15/8278A01N43/50C12Q1/6895C12Q2600/13C12Q2600/156
Inventor J·A·麦克尔利文B·辛格
Owner BASF PLANT SCI GMBH
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