Preparation method of immobilized aminoacylase and product and application thereof

An aminoacylase and amino acid technology, which is applied in the fields of immobilization on/in organic carriers, chemical industry, sustainable manufacturing/processing, etc., can solve the problems of high price, poor fluidity, and affecting enzyme activity, and achieve convenient Production operation, high mechanical strength, effect of lowering freezing point

Active Publication Date: 2010-03-17
滨海瀚鸿生化有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to overcome that the existing materials for immobilizing aminoacylase are expensive, can only be used in fixed bed reactions, are inconvenient for industrial operation, and are too high in freezing point to be difficult to immobilize and shape and affect the enzyme. activity, the immobilized glue is mostly large blocks or flakes, poor fluidity and other defects, and a preparation method of immobilized aminoacylase, immobilized aminoacylase and its preparation method are provided. application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Take 2 grams of carrageenan: gelatin at a ratio of 9:1, add 100ml of water, heat to dissolve, lower to 50°C, mix with 0.1g of aminoacylase, and pour into a kerosene flask at room temperature (25°C) 150rpm at a lower speed, stirred for 30 minutes, then filtered, washed once with distilled water, then added 0.1NKCL solution with a concentration of 0.5% glutaraldehyde, solidified for 2 hours, and prepared as immobilized enzyme particles.

Embodiment 2

[0026] Take 2 grams of carrageenan: gelatin at a ratio of 4:1, add 100ml of water, dissolve at 60°C, cool down to 40°C, mix with 0.02g of aminoacylase, and pour it into gasoline at room temperature (25°C) In the flask, stir at 150 rpm for 30 minutes, then filter, wash once with distilled water, then add 0.1 NKCL solution with a concentration of 0.5% glutaraldehyde, solidify for 2 hours, and prepare immobilized enzyme particles.

Embodiment 3

[0028] Take carrageenan: 2 grams of gelatin, the ratio is 19:1, add 198ml (198g) of water, dissolve at 75°C, drop to 50°C, mix evenly with 0.3g of aminoacylase, and flow to room temperature (25°C ) in a kerosene flask at a speed of 150 rpm, stirred for 30 minutes, then filtered, washed once with distilled water, then added 0.1NKCL solution with a concentration of 0.5% glutaraldehyde, solidified for 2 hours, and prepared as immobilized enzyme particles.

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PUM

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Abstract

The invention discloses a preparation method of immobilized aminoacylase, comprising the following steps: dissolving immobilizing materials (carrageenan and gelatin) in water at 60 to 90 DEG C, cooling to 40 to 60 DEG C, adding aminoacylase, uniformly mixing, adding the mixed liquid to an organic solvent with the temperature of 20 to 35 DEG C, and stirring and filtering to obtain granules; addingthe granules to a KCL water solution for immobilization to obtain the immobilized aminoacylase, wherein the KCL water solution also contains glutaraldehyde. The invention also discloses the immobilized aminoacylase prepared by the method and the application thereof. The preparation method of the invention can effectively reduce the solidifying points of the immobilizing materials, and the preparedimmobilized aminoacylase is in a granule shape, has good fluidity, and can effectively and conveniently resolve optically active amino acid.

Description

technical field [0001] The invention relates to a preparation method of an immobilized enzyme, its product and application, in particular to a preparation method of an immobilized aminoacylase, the immobilized aminoacylase prepared therefrom and its application. Background technique [0002] Aminoacylases are mainly used to split acylated-DL-amino acids into L-form and D-form optically active amino acids. Free enzymes can be used in the production of optically active amino acids, but the stability is poor, and the free enzymes can only be used in batch reactors. After the reaction, the enzymes cannot be recovered, which makes the separation and purification of products difficult and easily causes protein pollution. Therefore, it is very important for the production of unnatural amino acids So free enzyme is not an ideal catalyst yet. In order to overcome the various shortcomings of free enzymes, people have done a lot of research on the process and application technology of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/04C12P41/00C12P13/04
CPCY02P20/50
Inventor 马士忠李莉马道功元波马士强杨召鹏袁明张宁董龙辉
Owner 滨海瀚鸿生化有限公司
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