Plant CO2 sensors, nucleic acids encoding them, and methods for making and using them

A plant organ, plant technology, applied in the field of drought-resistant plants, to achieve the effect of enhanced carbon and water utilization

Inactive Publication Date: 2013-08-21
RGT UNIV OF CALIFORNIA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, no CO2 sensor has yet been identified

Method used

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  • Plant CO2 sensors, nucleic acids encoding them, and methods for making and using them
  • Plant CO2 sensors, nucleic acids encoding them, and methods for making and using them
  • Plant CO2 sensors, nucleic acids encoding them, and methods for making and using them

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 6

[0435]

[0436] a Arabidopsis genome project locus number

[0437] bAccording to Fabre N. et al., (2007) Plant, Cell Environment 30:617-629; or from the Arabidopsis information resource website (Carnegie Institution for Science, Department of Plant Biology, Stanford, CA, National Science Foundation grant) .

[0438] Thus, in alternative aspects, any carbonic anhydrase (carbonic dehydratase) may be used in the practice of the present invention.

[0439] Generating and Manipulating Nucleic Acids

[0440] In alternative aspects, the invention provides, for example isolated, synthetic and / or recombinant encoding novel CO of the invention 2 Nucleic acid and coding sequences of sensor genes that inhibit CO 2 Nucleic acids for sensor gene or messenger expression (eg siRNA, microRNA, antisense nucleic acids), and guard cell specific transcriptional regulatory elements such as promoters. Nucleic acids of the invention can be produced, isolated and / or manipulated by, for exampl...

Embodiment 1

[0496] Embodiment 1: By controlling the CO of the present invention 2 Sensor genetic manipulation of water and carbon dioxide (CO2) through plant stomata 2 )exchange

[0497] The present invention provides for controlling the CO of the present invention 2 Genetic manipulation of sensors via plant stomatal water and carbon dioxide (CO 2 ) method of exchange.

[0498] Construction of an Arabidopsis double mutant: According to cell-specific microarray analysis, this double mutant lacks the full-length expression of two homologous genes, which are highly expressed in wild-type guard cells.

[0499] Arabidopsis double mutants lack full-length expression of homologous genes that are highly expressed in wild-type guard cells, according to cell-specific microarray analysis. CO2Sen double mutants display impaired pair [CO 2 ] altered stomatal response, as determined by real-time gas exchange analysis; [CO 2 ] Change involves 365ppm CO from ambient 2 to an increased 800...

Embodiment 2

[0508] Example 2: Controlling plant CO 2 CO uptake and water use efficiency 2 Receptor Characterization

[0509]The present invention provides compositions and methods for the controlled opening and / or closing of plant stomata. Stomata are formed by pairs of guard cells in the leaf epidermis and are able to control plant water loss and carbon dioxide (CO 2 ) influx into plants. The present invention provides for the control of CO for photosynthetic carbon fixation 2 Compositions and methods for the amount of uptake and control of the amount of water lost through transpiration through these "controllable" stomata. The present invention provides a mechanism for providing guard cell signal transduction to sense CO 2 level, water status, light and other environmental stimuli to adjust stomatal aperture to optimize CO under different conditions 2 Compositions and methods for inflow, water loss and plant growth.

[0510] The present invention provides methods for ma...

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Abstract

The invention provides compositions and methods for manipulating the exchange of water and / or carbon dioxide (CO2) through plant stomata by controlling CO2 sensor genes. The invention provides compositions and methods for enhancing or optimizing biomass accumulation in a plant. The invention provides compositions and methods for opening or closing a stomatal pore on a guard cell in the epidermis of a plant. The invention provides compositions and methods for increasing or decreasing oxygenation efficiency and / or carbon fixation in a guard cell in the epidermis of a plant by manipulating expression of a ribulose-1,5-bisphosphate carboxylase / oxygenase. The invention provides promoters for regulating expression of a nucleic acid in a plant guard cell.

Description

technical field [0001] The present invention relates generally to plant molecular biology and cell biology. Specifically, the present invention provides the ability to express and control CO 2 Sensor genes, including the novel CO of the present invention 2 Sensor genes to manipulate water and / or carbon dioxide (CO 2 ) Compositions and methods for exchanging via plant stomata. The invention also provides drought-resistant plants; and methods of engineering plants and drought-resistant plants with improved water use efficiency. Background technique [0002] Stomata in plant leaf epidermis control plant water loss and CO 2 Influx from the environment into the plant. Carbon dioxide is taken up for photosynthetic carbon fixation, and water is lost through transpiration through stomata. Each stomata consists of a pair of specialized cells called guard cells, which can change the size of the stomata by controlling the expansion of the guard cells. An important shape in agri...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/09C12N15/29
CPCC12N15/8273C12N15/8222C07K14/415C12N15/8261C12Q1/6837Y02A40/146C12Y402/01001C12N9/88Y02P60/20C12N15/8218C12N15/8225
Inventor 朱利安·施罗德玛丽亚·伊斯拉尔松约瑟夫·M·库恩杨颖贞胡红红奥雷利安·布依松-多尼尔
Owner RGT UNIV OF CALIFORNIA
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