Lactobacillus acidophilus freeze-drying composite protectant and application method thereof

A technology of Lactobacillus acidophilus and protective agent, which is applied in the field of Lactobacillus acidophilus freeze-dried composite protective agent, can solve the problems of not meeting the requirements of long-term storage of Lactobacillus acidophilus, the survival rate is reduced, and the survival rate is not easy to reduce. , the effect of high survival rate and long storage period

Inactive Publication Date: 2010-04-14
李为忠
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the Lactobacillus acidophilus lyoprotectant can be used to obtain a higher freeze-drying survival rate, the survival rate is signi

Method used

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  • Lactobacillus acidophilus freeze-drying composite protectant and application method thereof
  • Lactobacillus acidophilus freeze-drying composite protectant and application method thereof

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0016] Compound protective agent solution formula: skimmed milk powder 10%, lactose 10%, glycerin 0.5%, sodium glutamate 2%, vitamin C 0.2%. All the above are mass fractions after being formulated into aqueous solutions, the same below.

[0017] Wherein, the skimmed milk powder is commercially available New Zealand skimmed milk powder, and the weight percent content is: 0.8% of fat, 33.4% of protein, 54.1% of lactose, and 3.5% of water. The skimmed milk powder used below is the same as above.

[0018] Composite protectant solution preparation

[0019] ① Weigh sodium glutamate and vitamin C according to the amount, add some distilled water to dissolve, and filter to sterilize;

[0020] ② Weigh skimmed milk powder, lactose and glycerin, add part of distilled water, heat to dissolve and mix well, and cool to room temperature;

[0021] ③Adjust the pH value to about 7.0 with NaOH solution;

[0022] ④ Sterilize at 115°C for 10 minutes and cool to room temperature;

[0023] ⑤ Ad...

example 2

[0025] Compound protective agent solution formula: skimmed milk powder 10%, lactose 10%, glycerin 0.3%, sodium glutamate 2%, vitamin C 0.2%, distilled water 77.5%.

[0026] Prepare composite protective agent solution according to the method of example 1.

example 3

[0028] Compound protective agent solution formula: skimmed milk powder 10%, lactose 12%, glycerin 0.6%, sodium glutamate 2.5%, vitamin C 0.2%, distilled water 74.7%.

[0029] Prepare composite protective agent solution according to the method of example 1.

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Abstract

The invention provides lactobacillus acidophilus freeze-drying composite protectant, and ingredients thereof includes: 9-11 parts by weight of dry buttermilk, 10-12 parts by weight of lactose, 0.3-0.6 part by weight of glycerol, 1.5-2.5 parts by weight of monosodium glutamate and 0.15-0.25 part by weight of Vitamin C. A freeze-drying protection method utilizing the lactobacillus acidophilus freeze-drying composite protectant includes steps of adding any type of lactobacillus acidophilus freeze-drying composite protectant into lactobacillus acidophilus liquor to generate protective liquor with the total weight of 130-180 parts by weight, and then freezing and drying. By utilizing the lactobacillus acidophilus freeze-drying composite protectant, higher freeze-driving survival rate of lactobacillus acidophilus is achieved with longer preservation, and the survival rate thereof is not easy to be reduced.

Description

technical field [0001] The invention relates to a freeze-drying compound protectant for Lactobacillus acidophilus and a freeze-dry protection method for Lactobacillus acidophilus using the protectant. Background technique [0002] Due to the continuous deepening of the research on Lactobacillus acidophilus, it has been confirmed that Lactobacillus acidophilus and its metabolites are beneficial to health. It can improve or regulate the balance of intestinal flora, enhance the body's immunity, lower cholesterol levels, relieve lactose intolerance, prevent and treat diarrhea, reduce allergic reactions, and prevent fungal infections, etc. Therefore, it is of great significance to develop Lactobacillus acidophilus products and supplement the number of Lactobacillus acidophilus colonized in the human intestinal tract through exogenous means to adjust the microecological balance of the human intestinal tract, improve immunity, and promote health. Now it has been used in food, heal...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/04C12R1/23
Inventor 王豫田
Owner 李为忠
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