Automatic in-situ monitor for detecting total content of microorganisms by adenosine triphosphate bioluminescence method
An adenosine triphosphate biological and microbial total technology, which can be used in biological material pretreatment, biochemical equipment and methods, enzymology/microbiology devices, etc., and can solve problems such as consuming a lot of manpower and material resources
Inactive Publication Date: 2012-11-28
ZHEJIANG UNIV
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These cannot be achieved with manual-based instruments or require a lot of manpower and material resources
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[0016] figure 1 This is the structural block diagram of the automatic in-situ monitor for detecting the total amount of microorganisms by adenosine triphosphate bioluminescence method. Computer, stepping motor and temperature control system, the computer communicates with the microprocessor in the acquisition control system through the communication interface circuit, sets the working parameters of the monitor and reads the collected data for analysis, and the acquisition control system follows the settings set by the computer The working parameters control the operation of each module of the monitor during in-situ monitoring, including controlling the stepping motor, controlling the working status of each valve in the valve group, controlling the temperature control system, collecting fluorescent signals in the plunger reaction chamber, etc., stepping Under the control of the acquisition control system, the motor can change the volume of the plunger reaction chamber, and coop...
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The invention discloses a design scheme of an automatic in-situ monitor for detecting total content of microorganisms by an adenosine triphosphate(ATP) bioluminescence method. The automatic in-situ monitor comprises a plunger type reaction chamber, a waste liquid chamber, a sample chamber, a cleaning liquid chamber, a reagent chamber, a valve bank, an acquisition control system, a computer, a step motor and a temperature control system, wherein the waste liquid chamber, the sample chamber, the cleaning liquid chamber, the reagent chamber, the reaction chamber and the acquisition control system are connected with the valve bank; the reaction chamber, the computer, the step motor and the temperature control system are connected with the acquisition control system respectively; the reagent chamber and the plunger type reaction chamber are connected with the temperature control system respectively; and the step motor is connected with the plunger type reaction chamber. The automatic in-situ monitor can accurately add a fluorescent reagent, adopts a semiconductor refrigerator to generate low temperature to store a biochemical reagent, performs the constant-temperature control on the plunger type reaction chamber, detects the fluorescent light excited by the ATP, directly estimates the content of the microorganisms according to the relationship between the ATP and the microorganisms, and is automatically cleaned after detection.
Description
technical field [0001] The invention relates to an automatic in-situ monitor for detecting the total amount of microorganisms by an adenosine triphosphate bioluminescent method, belonging to the field of optical detection instruments. Background technique [0002] In the fields of environmental testing, biology, oceanography and food hygiene, microbiological testing is an important project. Traditional microbial detection methods, such as culture counting, are also national standard methods. It takes 2 days to detect the total number of bacteria, and 3-5 days to detect yeast and mold, which is difficult to meet the requirements of online detection in terms of time. Moreover, for microorganisms that are difficult to cultivate or cannot be cultivated, it is difficult to apply this method to calculate the total number of viable bacteria. The ATP bioluminescent method has been paid more and more attention due to its advantages of simplicity, rapidity and automatic quantitative ...
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IPC IPC(8): C12M1/34G01N21/64
Inventor 叶树明杨俊毅蒋凯李顶立楼凯凯
Owner ZHEJIANG UNIV