Submerged fermentation culture method of straw mushroom liquid strain and culture medium thereof

A technology of deep fermentation culture and liquid culture medium, which is applied to the deep fermentation culture method of straw mushroom liquid strains and the culture medium field thereof, can solve the problems of low quality of mycelium, unfavorable growth of fungus balls, and lack of food, etc. The effect of shortening the incubation time

Inactive Publication Date: 2010-05-05
张纪明
View PDF4 Cites 23 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the annual production of Straw Mushroom factory, due to the extremely short production cycle of Straw Mushroom, the demand for strains is very large, so solid strains cannot meet the needs of Straw Mushroom factory annual production.
There are still many problems in the currently used fermented culture technology of straw mushroom liquid strains, specifically: (1) use a general-purpose medium to propagate the mother species, so the breeding time of the mother species is longer and the mycelium quality is not high; (2) more The solid strains cultured with the mother s

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0029] This embodiment is a preferred embodiment, and its specific steps are.

[0030] (1) Cultivate the mother species in the special mother species medium for straw mushrooms. Take 0.5cm 2 See square straw mushroom mother species, inoculate it into a special mother species culture medium, and culture it in the dark under the condition of 30±1°C, carry out strain activation and subculture, and form a test tube mother species. The content and the composition of the preparation of the special mother seed culture medium of this step are: apple juice (crushed juice) 200ml agar 30g straw mushroom fungus leaching solution 800ml; adjust pH to 7.4.

[0031] (2) Transplant the mother species mycelia that are full of test tubes in the mother species culture medium into the special medium for the original species of straw mushrooms and carry out transitional culture: the full bottle of mycelia that is gained from step (1) is transplanted into 150ml In the triangular flask, six bottles...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a submerged fermentation culture method of straw mushroom liquid strains and culture mediums thereof. The invention solves the technical defects of the prior art, such as long mother species propagation time, poor hypha quality, long fungus ball production time, different fungus ball sizes, higher production cost and the like. The method is used for the submerged fermentation culture of the straw mushroom liquid strains. The method comprises the following steps: 1. carrying out mother species culture in a special culture medium of straw mushroom mother species; 2. carrying out transition culture in a special culture medium of straw mushroom stocks; 3. homogenizing the stocks to obtain homogeneous succulent strains; 4. carrying out primary shaking culture and amplification in a shaking liquid culture medium; 5. carrying out secondary shaking culture and amplification; and 6. carrying out fermentation treatment in a submerged fermentation liquid culture medium to obtain the finished products of the straw mushroom liquid strains. The invention relates to the special culture medium of straw mushroom mother species, the special culture medium of straw mushroom stocks, the shaking liquid culture medium and the submerged fermentation liquid culture medium.

Description

Technical field: [0001] The invention relates to a submerged fermentation culture method and a culture medium thereof, in particular to a submerged fermentation culture method and a culture medium of straw mushroom liquid strains. Background technique: [0002] Straw mushrooms have been produced using solid strains. In the annual production of Straw Mushroom factory, because the production cycle of Straw Mushroom is very short, so the demand for strains is very large, so solid strains can not meet the needs of the annual production of Straw Mushroom factory. There are still many problems in the currently used fermented culture technology of straw mushroom liquid strains, specifically: (1) use a general-purpose medium to propagate the mother species, so the breeding time of the mother species is longer and the mycelium quality is not high; (2) more The solid strains cultured with the mother seed medium are directly cultured in a first-level shake flask without homogenization...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01G1/04C05G1/00C05F11/08
Inventor 张纪明
Owner 张纪明
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products