Method for quickly propagating grassiness

Abstract

The invention relates to a method for quickly propagating grassiness, belonging to the technical field of plant cell engineering. By taking young spikes of grassiness as materials, the grassiness cell engineering seedlings are quickly propagated by callus induction, clump bud induction and clump bud multiplication and root-strengthened culture; one young spike can generate 85 to 406 cell engineering seedlings by expanding propagation once; the average propagation coefficient of the cell engineering seedling is 198 plants/spike; the transplanting survival rate for cell engineering seedling greenhouse or field seedbed is 98%; and after the growth for more than 30 days, the young spikes can be taken as seedlings for field planting. The method can overcome the defect that the known method for regenerating the culture plants of grassiness cells can not induce the clump buds; furthermore, the method in the invention is a beneficial supplementation for the existing technologies of asexual propagation of grassiness seed stems and propagation-expanding of cell engineering of seed stems, has the advantages of having high propagation coefficient, conveniently obtaining materials, simple and convenient operation, and provides technical supports for the industrial application of the grassiness.

Description

1. Technical field [0001] The invention relates to a rapid propagation method of elephant grass, which belongs to the technical field of plant cell engineering. 2. Background technology [0002] Elephant Grass (Pennisetum Purpureum Schumach), also known as Pennisetum Purpureum Schumach, is a perennial herbaceous plant of the Gramineae Pennisetum genus. It is native to tropical Africa and is suitable for cultivation in tropical and subtropical regions. Elephant grass can not only be used as high-quality green feed for herbivorous livestock and poultry and aquatic fish, ideal raw material for high-quality pulp and medium-to-high-end composite wood-based panels, but also an important biomass energy crop. The planting area at home and abroad is expanding rapidly. [0003] Although Elephant Grass is a perennial herb, it cannot bloom and bear fruit naturally in the middle and lower reaches of the Yangtze River in my country and areas at the same latitude. Propagation usually adopt...

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Problems solved by technology

[0003] Although Elephant Grass is a perennial herb, it cannot bloom and bear fruit naturally in the middle and lower reaches of the Yangtze River in my country and areas at the same latitude. Propagation usually uses stem nodes that have grown for about 2 months as materials for vegetative propagation, which has the disadvantages of low reproduction coefficient and labor-intensive operations.

Jiang Hongru et al. used the young tender stem nodes of Dwarf Elephant Grass as explants to establish the test tube rapid propagation technology system of Dwarf Elephant Grass (such as Jiang Hongru, She Faxin, Wang Biqin, Liu Tengyun. Research on the Test Tube Rapid Propagation Technology of Dwarf Elephant Grass. The full-text database of important conferences in China) has effectively utilized the young stem nodes and has a high reproduction coefficient; Zhong Xiaoxian et al. used the young panicles of Elephant grass as explants to establish the "Plant Regeneration Method of Elephant Grass Somatic Cell Culture" (ZL 200610085256.2), It provides the possibility to use the young panicles of Elephant grass to propagate seedlings, but there are shortcomings that the existing young panicles cannot induce clustered seedlings and the reproduction coefficient is low

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