Human IgG and rheumatoid factor absorption device and related detection method of IgM antibody in human serum

A technology of rheumatoid factor and adsorption device, which is applied to the detection of IgM antibody in human serum, and the field of human IgG and rheumatoid factor adsorption device, can solve the problems of false negative, poor IgG and RF removal effect, and poor ability to adsorb RF, To achieve the effect of strong adsorption capacity, suitable for large-scale promotion and application, and improved accuracy

Inactive Publication Date: 2010-05-12
无锡博慧斯生物医药科技有限公司
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] ①In the serum of patients with previous infection and chronic patients, the competitive adsorption of specific IgG antibody and specific IgM in the sample to be tested caused false negatives;
Ordinary IgG is also used as an adsorbent; however, the ability to adsorb RF is p

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0030] The coating of embodiment 1 microwell plate

[0031] Use 50ul goat anti-human IgG monoclonal antibody per well and 50ul glutaraldehyde cross-linked denatured human IgG coating buffer (NaCl 8.0g, KH2PO4 0.2g, Na2HPO4.12H2O 2.9g, KCl 0.2g, dissolved in 1000ml water) was coated at 4°C for 24 hours, then sealed with skimmed milk powder for 24 hours, washed, air-dried, sterilized and sealed for later use.

Example Embodiment

[0032] Embodiment 2 is to human IgG in serum and rheumatoid factor (RF) adsorption test

[0033] Add 50ul of positive and negative clinically tested serum samples to the above-mentioned coated microwell plate, incubate in a 37°C incubator for 30 minutes, then take out the serum sample in the microwell plate for detection; use non-adsorbent The serum samples were used as a control; the results showed that the detection accuracy of the adsorbed serum samples was 99.2%; while the detection accuracy of the unadsorbed serum samples was 78.6%.

[0034] In summary, the human IgG and rheumatoid factor adsorption device of the present invention can specifically adsorb human IgG and rheumatoid factor, thereby easily and quickly eliminating the interference of human IgG and rheumatoid factor, facilitating rapid and accurate realization of related detection, suitable for It is suitable for large-scale promotion and application, and provides guarantee for simple and rapid large-scale clini...

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Abstract

The invention provides a human IgG and a rheumatoid factor absorption device comprising a microplate which comprises at least one micropore, substances for specifically absorbing human IgG and rheumatoid factors are coated in the micropore; preferably, the microplate is prepared from polystyrene or polypropylene, and the number of the micropores ranges from 1 to 1536; the microplate also comprises a base, and can be arranged on the base in a dismountable manner; and the substances are goat anti-human IgG antibody and denatured/agglutinative human IgG. The invention also provides a method using the above device to detect IgM antibody in human serum. The human IgG and rheumatoid factor absorption device can specifically absorb human IgG and rheumatoid factors, thereby conveniently and rapidly removing the interference of human IgG and rheumatoid factors, being beneficial to the rapid and accurate realization of related detection, being applicable to large scale promotion and application, and providing guarantee for conveniently and rapidly performing large-scale clinical detection.

Description

technical field [0001] The present invention relates to the technical field of immunology, more specifically, to the technical field of devices for improving the accuracy of immunological detection, in particular to an adsorption device for human IgG and rheumatoid factor and a related method for detecting IgM antibody in human serum. Background technique [0002] The detection of IgM antibodies is interfered to varying degrees by specific IgG and rheumatoid factor (RF). Especially in the detection of IgM antibodies in the TORCH series (toxoplasma gondii, rubella virus, cytomegalovirus, herpes simplex virus, etc.) during pregnancy, due to the presence of more IgG and rheumatoid factors, the detection of IgM is more likely to be interfered. [0003] The main reason for the inaccurate detection of IgM ELISA (indirect method) is: there are specific IgG antibodies and IgM type RF in the serum, and the specific reasons are as follows: [0004] ①In the serum of patients with prev...

Claims

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Application Information

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IPC IPC(8): G01N33/543G01N33/545
Inventor 韦彦余张丽新雷震
Owner 无锡博慧斯生物医药科技有限公司
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