Method for preparing water purification agent for degrading nitrite and ammoniacal nitrogen in aquaculture water body

Abstract

The invention discloses a method for preparing a water purification agent for degrading nitrite and ammoniacal nitrogen in an aquaculture water body. The method comprises the steps of: performing activated culture on Bacillus cereus on an activated liquid culture medium for two times to try to ensure that cells are at a synchronous growth stage, and then inoculating a working fermentation agent into an enriched medium according to the volume ratio of 1-10: 100 to culture for 24 to 48h under the condition that the temperature is between 25 and 40 DEG C and the rotating rate is between 150 and 200r / min to ensure that the viable count is over 1,014cfu / ml; or utilizing vacuum hot-air drying to ensure that the viable count reaches over 1,016cfu / g. The preparation has the ability of efficiently degrading nitrite, ammoniacal nitrogen and COD in a water body in freshwater and seawater aquaculture, and has good effect of clarifying the aquaculture water body.

Description

technical field [0001] The invention relates to a water purifier for degrading nitrite and ammoniacal nitrogen in aquaculture water cultivated by non-pathogenic bacillus cereus. Background technique [0002] In recent years, the aquaculture industry has developed rapidly, from traditional open-type farming methods to semi-intensive, even intensive, facility-based, and intensive farming methods. The progress of aquaculture methods is a "double-edged sword". On the one hand, the production of aquatic products can be greatly increased, on the other hand, the damage to the environment of the aquaculture water body is increasingly aggravated, that is, high-density aquaculture and a large amount of artificial bait are used to rapidly deteriorate the quality of the aquaculture water body, which seriously affects the production, quality and breeding benefits of aquatic products. . Under this high-density breeding mode, the residual bait, excrement and other organic pollutants in th...

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Problems solved by technology

[0003] There are two types of water purifiers for aquaculture water, one is based on inorganic salts, this method can solve some problems in a short period of time, but it cannot fundamentally solve the problem of excessive nitrite nitrogen and ammonia nitrogen in aquaculture water problem; the other is based on micro-ecological live bacteria, and in the current method, there are generally three defects:

[0004] (1) The number of live bacteria is too low or the number of live bacteria is too small, and the action time is too long, which can't achieve immediate results. As the application number is 200810100231.4, the Chinese invention patent application discloses "composite live bacteria biological water purifier and The total number of bacteria in its preparation method" is 10 8 cfu / g, due to the low total number of bacteria, the effect of degrading nitrite and ammoniacal nitrogen in the water body is not obvious; the Chinese invention patent application with the patent number ZL 200410024622.4 discloses the method of "preparation of detoxification bacteria and restoration of aquaculture environment" The fermentation period is as long as 15 days, and the number of viable bacteria is only 10 8 cfu / g, used for detoxification of aquaculture water for a long time, can not solve the urgent need; the Chinese invention patent application with application number 02134998.3 discloses "a long-acting microbial water purifier and its production method", in which the total number of bacteria is only up to 10 9 cfu / ml, and the bacterial strain Geotrichum candidum AS2.1175 is also used, and its optimum temperature for growth is narrow and lower at about 20°C, which is not suitable for changes in the temperature of the aquaculture water body; the Chinese invention patent application with the application number 00117453.3 discloses "a A high-density live bacteria fermentation process for live bacteria biological water purifiers”, in which the nitrogen source of the fermentation medium is mainly inorganic nitrogen sources, which cannot meet the large demand for nitrogen sources of vigorously growing spores, making it impossible for the amount of live bacteria to reach True "High Density"

[0005] (2) Use raw materials with strong adsorption functions as immobilized carriers, or micro-ecological water purifiers made into tablets, so that the spores can easily sink to the bottom of the aquaculture water when they are released, and cannot provide the spores necessary for reproduction Oxygen, so the effect is not significant

For example, the Chinese invention patent application whose application number is 200810198302.9 discloses "a process for immobilized nitrifying bacteria to degrade aquaculture wastewater nitrite", which is one of them; the Chinese invention patent application whose application number is 200510112131. Nitrate degrading agent and production method ", use sodium alginate-calcium chloride to embed Bacillus subtilis and nitrifying bacteria. The water body causes certain pollution; the Chinese invention patent application with the application number 200710059467.3 discloses "a compound microecological preparation, dosage form and preparation process for purifying aquaculture water", which uses modified polyacrylamide, which is not easy to degrade Potential hazards. When flocculated to a certain extent, larger molecular particles can be formed and sink to the bottom of the pond, or be swallowed by filter-feeding fish, causing certain potential hazards

[0006] (3) The effect of the selected living bacteria degrading nitrite and ammoniacal nitrogen in the aquaculture water body is not obvious, the scope of action is narrow, and the effect is single. For example, the Chinese invention patent application with application number 200410014054.X discloses "composite live bacteria "Preparation method of biological water purifier", this method is to prepare dry powder by culturing Bacillus cereus, Bacillus subtilis and Bacillus licheniformis. Only the preparation process is announced, and the use effect is not announced. The required high-efficiency and rapid intensive farming is not enough; the Chinese invention patent application with application number 200710193008.4 discloses "enhanced microbial preparations for regulating water quality in freshwater aquaculture and its application", which is only applicable to freshwater aquaculture and does not involve seawater The scope of aquaculture is not comprehensive; the Chinese invention patent application with the application number 200610013732.X discloses "microbial preparations for aquaculture", and the bacteria used have no flocculation effect, so they cannot quickly and efficiently clarify the aquaculture water body

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