Method for preparing water purification agent for degrading nitrite and ammoniacal nitrogen in aquaculture water body

Abstract

The invention discloses a method for preparing a water purification agent for degrading nitrite and ammoniacal nitrogen in an aquaculture water body. The method comprises the steps of: performing activated culture on Bacillus cereus on an activated liquid culture medium for two times to try to ensure that cells are at a synchronous growth stage, and then inoculating a working fermentation agent into an enriched medium according to the volume ratio of 1-10: 100 to culture for 24 to 48h under the condition that the temperature is between 25 and 40 DEG C and the rotating rate is between 150 and 200r/min to ensure that the viable count is over 1,014cfu/ml; or utilizing vacuum hot-air drying to ensure that the viable count reaches over 1,016cfu/g. The preparation has the ability of efficiently degrading nitrite, ammoniacal nitrogen and COD in a water body in freshwater and seawater aquaculture, and has good effect of clarifying the aquaculture water body.

Description

technical field [0001] The invention relates to a water purifier for degrading nitrite and ammoniacal nitrogen in aquaculture water cultivated by non-pathogenic bacillus cereus. Background technique [0002] In recent years, the aquaculture industry has developed rapidly, from traditional open-type farming methods to semi-intensive, even intensive, facility-based, and intensive farming methods. The progress of aquaculture methods is a "double-edged sword". On the one hand, the production of aquatic products can be greatly increased, on the other hand, the damage to the environment of the aquaculture water body is increasingly aggravated, that is, high-density aquaculture and a large amount of artificial bait are used to rapidly deteriorate the quality of the aquaculture water body, which seriously affects the production, quality and breeding benefits of aquatic products. . Under this high-density breeding mode, the residual bait, excrement and other organic pollutants in th...

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Problems solved by technology

[0003] There are two types of water purifiers for aquaculture water, one is based on inorganic salts, this method can solve some problems in a short period of time, but it cannot fundamentally solve the problem of excessive nitrite nitrogen and ammonia nitrogen in aquaculture water problem; the other is based on micro-ecological live bacteria, and in the current method, there are generally three defects:

[0004] (1) The number of live bacteria is too low or the number of live bacteria is too small, and the action time is too long, which can't achieve immediate results. As the application number is 200810100231.4, the Chinese invention patent application discloses "composite live bacteria biological water purifier and The total number of bacteria in its preparation method" is 10 8 cfu/g, due to the low total number of bacteria, the effect of degrading nitrite and ammoniacal nitrogen in the water body is not obvious; the Chinese invention patent application with the patent number ZL 200410024622.4 discloses the method of "preparation of detoxification bacteria and restoration of aquaculture environment" The fermentation period is as long as 15 days, and the number of viable bacteria is only 10 8 cfu/g, used for detoxification of aquaculture water for a long time, can not solve the urgent need; the Chinese invention patent application with application number 02134998.3 discloses "a long-acting microbial water purifier and its production method", in which the total number of bacteria is only up to 10 9 cfu/ml, and the bacterial strain Geotrichum candidum AS2.1175 is also used, and its optimum temperature for growth is narrow and lower at about 20°C, which is not suitable for changes in the temperature of the aquaculture water body; the Chinese invention patent application with the application number 00117453.3 discloses "a A high-density live bacteria fermentation process for live bacteria biological water purifiers”, in which the nitrogen source of the fermentation medium is mainly inorganic nitrogen sources, which cannot meet the large demand for nitrogen sources of vigorously growing spores, making it impossible for the amount of live bacteria to reach True "High Density"

[0005] (2) Use raw materials with strong adsorption functions as immobilized carriers, or micro-ecological water purifiers made into tablets, so that the sp...

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