Device for directly seeding and culturing arabidopsis thaliana in water and application thereof

A hydroponic device and an Arabidopsis technology are applied in the field of devices for direct seeding hydroponic Arabidopsis, which can solve the problems of weak Arabidopsis seedlings, affecting the survival rate, easy root damage, etc., so as to improve the germination rate and survival rate, Improve the survival rate and the effect of easy raw materials

Inactive Publication Date: 2010-06-16
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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Problems solved by technology

Arabidopsis seedlings are weak, and their root system is easily damaged when transplanting, which affects the survival rate. Especially when performing high-throughput screening of Arabidopsis mutants, thousands of seedlings need to be transplanted, and the workload is very heavy

Method used

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  • Device for directly seeding and culturing arabidopsis thaliana in water and application thereof
  • Device for directly seeding and culturing arabidopsis thaliana in water and application thereof

Examples

Experimental program
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Embodiment 1

[0026] Embodiment 1, the cultivation of Arabidopsis

[0027] Now use the device of the present invention to cultivate Arabidopsis thaliana in an artificial climate chamber, comprising the following steps:

[0028] 1) Vernalization treatment of Arabidopsis thaliana seeds: Arabidopsis thaliana seeds of ecotype Wassilewskija (Ws) were placed in a centrifuge tube, and placed in a refrigerator at 4° C. for 3 days in the dark.

[0029]2) An opaque plastic basin (38x27x14cm) is an open container in this device; a white foam board is used to make a support plate; a 1000 μL pipette tip is used to make a hollow tube. Cut off the thin end of the 1000 μL tip by 4 cm, and place the remaining part (about 3 cm) upside down on the glass plate to fill the growth gel with a culture solution containing 8 mg / ml agar and a concentration of one-tenth of the Hoagland nutrient solution ; Punch a well-cut white foam plate, the hole diameter matches the 1000μL suction head, and the distance between th...

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Abstract

The invention discloses a device for seeding directly and culturing arabidopsis thaliana in water and the application thereof. The device comprises an open container; a supporting flat plate is arranged in the open container; one or more small holes are disposed on the supporting flat plate; a hollow pipe is inserted in each small hole; solid culture medium is filled in each hollow pipe; water or nutrient solution is filled in the open container; the supporting flat plate is arranged above the level of the water or the nutrient solution; the upper end of each hollow pipe is arranged above the supporting flat plate; and the lower end of each hollow pipe is inserted in the water or the nutrient solution. The device is used for seeding directly and culturing the arabidopsis thaliana in water. The method and the device are simple, are easy to obtain the raw materials and can obtain a large number of plant organs such as roots, stems and leaves used for the biochemical and physiological research.

Description

technical field [0001] The invention relates to a hydroponic device for Arabidopsis thaliana, in particular to a device for live-seeding Arabidopsis thaliana in an artificial climate chamber and its application. Background technique [0002] Arabidopsis thaliana is a plant of the genus Arabidopsis in the Brassicaceae family. It has the characteristics of short growth period, small plant and small genome. It has been used as a model experiment material for molecular biology and genetics research for a long time, especially It plays a very important role in the research of plant developmental biology and is known as the "fruit fly" of the plant kingdom. However, Arabidopsis seeds are very small and the seedlings are very weak, which brings certain difficulties to its cultivation; and its volume and biomass are correspondingly small, so that the research on some physiological and biochemical problems is limited. Experiments with Arabidopsis thaliana are the basis for the succe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G31/02A01G31/00C05G1/00
CPCY02P60/21
Inventor 孙秀梅张纪秀陈吉平关亚风
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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