Dominant dwarf rice related protein, encoding gene thereof and application

A technology encoding genes and proteins, which can be used in applications, genetic engineering, plant genetic improvement, etc., and can solve problems such as weakening the inhibitory effect of GA signal transduction

Active Publication Date: 2010-06-23
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

C-terminal mutations make SLR1 lose or weaken the inhibitory effect on GA signal transduction, but the mutant gene product can still sense GA signals normally and trigger its own degradation, so the slr1 mutation is a recessive mutation

Method used

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  • Dominant dwarf rice related protein, encoding gene thereof and application
  • Dominant dwarf rice related protein, encoding gene thereof and application
  • Dominant dwarf rice related protein, encoding gene thereof and application

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Experimental program
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Effect test

Embodiment 1

[0045] Embodiment 1, the isolation of gene

[0046] 1. Phenotype analysis of dwarf mutants

[0047] The applicant of the present invention discovered a dominant dwarf mutant (named Slr1-d4) in the indica rice variety Zhongxian 3037, and crossed the mutant with a normal line (Zhongxian 3037 wild type) to obtain the F1 generation. The plant height of the F1 generation was between the mutant parent and the wild parent, showing good plant height traits and no other bad traits.

[0048] Under normal cultivation conditions, the plant type of the rice mutant Slr1-d4 was significantly different from that of the wild type Zhongxian 3037 ( figure 1 , A represents the wild type Zhongxian 3037 (slr1-d4 / slr1-d4), B represents the heterozygote between Zhongxian 3037 and the mutant (Slr1-d4 / slr1-d4), C represents the homozygous mutant (Slr-d4 / Slr-d4)), mainly manifested in the obvious dwarfing of the mutant, the average final plant height of the mutant (Slr-d4 / Slr-d4) is about 47.6cm, whi...

Embodiment 2

[0074] Embodiment 2, the function of gene

[0075] 1. Functional complementation of dwarfing gene Slr1-d4

[0076] (1) Acquisition of recombinant expression vectors

[0077] The plant expression vector pCAMBIA1300 vector was purchased from Australia, Canberra, Cambia.

[0078] The pCAMBIA1300 vector was double-digested with restriction endonucleases Xmal and Smal. The digested product was ligated with the full-length Slr1-d4 obtained by double digestion with Xmal and Smal, and a complementary plasmid containing the full-length Slr1-d4 gene fragment was obtained. Restriction digestion and sequencing verification showed that the sequence of the gene in the complementary plasmid was constructed and the insertion direction are correct (the sequence of the gene inserted in the complementary plasmid is shown in the 121-6237th nucleotide from the 5' end of sequence 2 in the sequence table), and the positive complementary plasmid is recorded as the recombinant expression vector pCAM...

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Abstract

The invention discloses dominant dwarf rice related protein, an encoding gene thereof and application. The protein is the following a) or b) protein: a) the protein which comprises an amino acid sequence shown in a sequence 1 in a sequence list; b) the protein which comprises the amino acid sequence shown in the sequence 1 in the sequence list, is through the replacement and / or the missing and / or the adding of one or more amino acid residues, is related to the character of a dwarf plant, and is derived from a). A mutant gene Slrl-d4 provided by the invention is mutated from a single base of a TVHYNP motif on the N terminal of a gene SLRl (SLENDERRICEl). The mutant gene is a dominant gene, can lead a rice plant to produce the character of the dwarf plant, so as to facilitate the improvement of the rice yield; the height of an F1 generation plant obtained by the hybrid of the mutant containing the gene and a normal wild-type indica rice 3037 is between that of the mutant parent and the wild parent, and the F1 generation plant shows good plant height and does not have other bad character. Therefore, the mutant gene is a novel dwarf plant resource with excellent economical character, and will display a significant role in the breeding of the plant, in particular the rice.

Description

technical field [0001] The invention relates to a rice dominant dwarf trait-related protein and its coding gene and application. Background technique [0002] In the 1960s and 1970s, due to the promotion of semi-dwarf varieties, the world's rice and wheat production achieved a breakthrough growth. This dwarf breeding is known as a "green revolution" in modern grain production. In rice production, what set off this "green revolution" was the application of the rice semi-dwarf gene sd1. SD1 encodes gibberellin 20-oxidase, which is involved in the synthesis of gibberellin. At present, most of the dwarf genes used in rice breeding are alleles of sd1. There are mainly 6 dwarf resources used in my country: Banantan Nante, Aizaizhan, Diaojiaowujian, Banjiazai, Hualong Shuitiangu and Indonesian Shuitiangu. The dwarf characteristics of these six varieties are determined by sd1 control. The singleness of this dwarf source gene can reduce the genetic diversity of cultivated rice, w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/11C12N15/63C12N1/00C12N5/10C12N15/82
Inventor 程祝宽李明苗春波唐丁王克剑顾铭洪
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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