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Microbial carrier and preparation method thereof

A microbial carrier and microbial technology, applied in the direction of immobilization on/in the organic carrier, can solve the problems of decreased diffusion behavior, difficulty in taking into account material stability, activity and diffusion behavior, etc., to achieve improved diffusivity, increased activity and diffusion Behavior, effect of good stability

Inactive Publication Date: 2010-06-23
SOUNDGRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the higher the degree of cross-linking of the current polymer network, the better the stability of the material, and the better the strength and stability of the formed carrier, but its diffusion behavior drops sharply. Therefore, it is difficult for the existing microbial carrier to take into account the stability of the material. Sexuality, microbial and / or enzymatic activity and diffusion behavior

Method used

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Examples

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Effect test

preparation example Construction

[0027] The preparation method of the microorganism carrier of the embodiment of the present invention is specifically as follows:

[0028] Get each raw material according to above-mentioned microbial carrier formula;

[0029] Add the water-soluble monomer capable of radical polymerization, water-soluble or water-dispersible polymer, microorganism and / or enzyme, water, cross-linking agent and radical polymerization initiator into the container according to the proportion, mix well, and adjust the temperature between 0 and 50 After standing at a constant temperature of ℃ for 2 minutes to 2 hours, the gel formed by polymerization is taken out of the container, cut into nearly cubes, and the microbial carrier is obtained.

[0030] The microbial carrier of the embodiment of the present invention is manufactured under anaerobic environment, but it is also suitable for aerobic microorganisms, facultative microorganisms and microorganisms that can sleep for more than 2 hours or surviv...

Embodiment 1

[0034] This embodiment 1 provides a microbial carrier, which is a hydrogel material with an interpenetrating network structure, and the hydrogel material is composed of a main network part and an interpenetrating part, wherein the main network part is The main body network is formed by free radical polymerization of water-soluble monomers; the interpenetrating part is formed by the presence of water-soluble or water-dispersible polymers with interactive groups in the form of linear or physical cross-linking to form an interpenetrating network.

[0035] The preparation method of the microbial carrier is as follows:

[0036] Get each raw material according to the following ratio:

[0037]Acrylamide (water-soluble monomer capable of radical polymerization) 6wt%

[0038] Polyvinyl alcohol (water-soluble or water-dispersible polymer) 2wt%

[0039] Ammonium persulfate / sodium bisulfite (initiator) 0.05wt%

[0040] N, N-methylenebisacrylamide (crosslinking agent) 0.1wt%

[0041] P...

Embodiment 2

[0046] This embodiment 2 provides a microbial carrier, which is a hydrogel material with an interpenetrating network structure;

[0047] The preparation method of the microbial carrier is as follows:

[0048] Get each raw material according to the following ratio:

[0049] Acrylamide (water-soluble monomer capable of radical polymerization) 8wt%

[0050] Sodium polyacrylate (polymer) 0.15wt%

[0051] Ammonium persulfate / sodium bisulfite (initiator) 0.08wt%

[0052] N, N-methylenebisacrylamide (crosslinking agent) 0.2wt%

[0053] Aqueous dispersion of nitrifying bacteria (water dispersion of microorganisms and / or enzymes, wherein the content of microorganisms and / or enzymes is 5wt% by solid, and the remaining 25wt% is water) 30wt%

[0054] Sterile water 61.55wt%

[0055] Glass powder 0.02wt%;

[0056] The preparation method is as follows: add water-soluble monomer, polymer, water dispersion of nitrifying bacteria, sterile water, cross-linking agent and glass powder into a...

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Abstract

The invention relates to a microbial carrier and a preparation method thereof. The microbial carrier is a hydrogel which can interpenetrate the network and is prepared by polymerization equipment; an interpenetrating network structure of the hydrogel comprises a main body network part and an interpenetrating part, wherein the main body network part is formed by water-soluble monomers through radical polymerization; and the interpenetrating part exists by macromolecules with interaction radicals in a linear or cross-linking form and respectively forms a full-interpenetrating network and a half-interpenetrating network. The compression ratio of the microbial carrier with the interpenetrating network structure in the embodiment of the invention can reach 95 percent. The microbial carrier is simple, convenient and feasible to embed microorganism. The obtained microorganism has stable quality and excellent activity and diffusivity.

Description

technical field [0001] The invention relates to the field of immobilized biology, in particular to a microbial carrier and a preparation method thereof. Background technique [0002] Immobilized Biotechnology (Immobilized Biotechnology) is a new technology that has developed rapidly since the 1960s. It uses chemical or physical means to locate free cells or enzymes in a limited space area, so that free cells or enzymes Remain active and can be used repeatedly. Immobilized biotechnology has the advantages of high microbial density, rapid reaction, less microbial loss, easy separation of products, and easy control of the reaction process. It is a promising wastewater treatment technology with high efficiency, low consumption, easy operation and management. In the past 20 years, immobilized biotechnology has developed rapidly and has achieved phased results, especially in the United States and Japan, where industrial examples with a certain scale have appeared. [0003] In im...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/08
Inventor 李宗慧屈佳玉王凯佟娟牛薇
Owner SOUNDGRP CO LTD
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