Polynucleotide sequences encoding scorpion toxin and transformed insecticidal fungi

A gene and neurotoxin technology, applied in the field of genetic engineering, can solve the problems of hindering the large-scale application of microbial pesticides and slow insecticidal effect.
CN101755050BInactive Publication Date: 2013-11-20SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
Publication Date
2013-11-20
Estimated Expiration
Not applicable · inactive patent

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Abstract

Provided are optimized polynucleotide sequences encoding a scorpion Androctonus australis neurotoxin AaIT, which are suitable for being transcribed and expressed in fungi. Also provided are the fungi capable of expressing AaIT, such as Metarhizium anisopliae, which have improved insecticidal efficiency and can be used for insect control in effect.
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Description

technical field

[0001] The invention belongs to the field of genetic engineering, and more particularly, the invention relates to an optimized polynucleotide encoding the North African scorpion neurotoxin polypeptide AaIT, a fungal engineering strain capable of expressing the North African scorpion neurotoxin polypeptide AaIT, and using the strain to kill Insect approach. Background technique

[0002] With the development of social economy and the improvement of people's living standards, the research and application of environmentally friendly microbial pesticides have received more and more attention. There are many kinds of microbial pesticides, and more than 2,000 kinds have been found. According to the types of microorganisms, they can be divided into bacteria, fungi, viruses, protozoa and nematodes. At present, there are mainly bacterial insecticides, fungal insecticides, and virus insecticides that have been developed, applied and commercialized.

[0003] Fungal ins...

Claims

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