Method for extracting and separating cucurbitacin B

Abstract

The invention discloses a method for extracting and separating cucurbitacin B, which comprises the following steps: firstly, melon pedicels as a raw material are crushed and are refluxed and extracted by ethanol diacolation or ethanol, an extracting solution is extracted by ethyl acetate more than three times after being concentrated, and the ethyl acetate is recovered to obtain a total cucurbitacin extract; secondly, the total cucurbitacin extract is taken to be dissolved in a 25 to 45 percent of ethanol water solution, and the hydrolysis reaction is carried out in the presence of acetic acid; the pH value is adjusted to be neutral after full reaction, and the extract is concentrated to dry; and then the obtained extract is treated by purification so as to obtain a cucurbitacin B product with more than 60 percent of cucurbitacin B content, and the concentration of the acetic acid is 0.1 to 0.3 mol / L. Compared with the prior art for extracting and separating cucurbitacin B, the invention has the following advantages: the analogue (such as cucurbitacin B glucoside) of the cucurbitacin B is converted into the cucurbitacin B by the structural transformation in the process of extraction, so that the extraction rate of the cucurbitacin B is improved, the production process is simple, and the operation is simple.

Description

technical field

[0001] The invention relates to the extraction and separation of active ingredients of natural products, in particular to a method for the extraction and separation of cucurbitacin B. Background technique

[0002] Cucurbitacin B is a cucurbitane substance distributed in a variety of plants. People have found the distribution of cucurbitacin B in muskmelon, gourd, Baixie root and other plants. However, the traditional cucurbitacin B extraction techniques mainly include: ethanol thermal extraction method, ultrasonic ethanol extraction method, etc. In these extraction and separation methods and technologies, the technical principles are based on the physical and chemical properties of target compounds with biological activity. The corresponding separation method and technology is a physical process in the whole process, which does not consider the comprehensive utilization of all chemical components of natural products, so the resource utilization rate is low, t...