Method utilizing hop residues to extract xanthohumol

A technology for hops and xanthohumol, which is applied in the separation/purification of carbonyl compounds, bulk chemical production, organic chemistry, etc., can solve problems such as easy loss, and achieve the effects of convenient extraction, high product purity and high yield

Inactive Publication Date: 2010-07-07
刘玉英
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But because the content of xanthohumol in hops is less than 1%, and its activity is higher, it is easy to lose in the process of boiling beer wort. Therefore, if people want to increase their intake, they can only It is extracted and added to beer or used as medicine
Currently, supercritical CO 2 In the process of extracting hop extract, hop polyphenols are basically not extracted, and the remaining hop residue is mostly discarded except for a small amount as feed, fertilizer, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Take 100g of hop waste residue, add 300mL of 95% edible ethanol and ultrasonically extract at 40°C for 1 hour, filter the extract, and repeat the extraction of the residue twice; combine the three extracts, concentrate under reduced pressure to remove most of the solvent, and obtain 25mL of a concentrate. Add 80g of diatomaceous earth into the concentrated solution, stir evenly, make the hop polyphenol extract evenly adsorb on the adsorbent diatomite, place it, and volatilize a small amount of solvent therein. Take 200mL of 30% methanol aqueous solution to elute the diatomite adsorbed with hop polyphenol extract, filter and separate the methanol aqueous solution for later use; then use 400mL of 60% methanol aqueous solution to elute the diatomite, and collect the obtained methanol aqueous solution by filtration 80mL of deionized water was added to the aqueous methanol solution under stirring, and the solution was left to stand. A yellow precipitate precipitated out of th...

Embodiment 2

[0026] Take 120g of hop waste residue, add 300mL of acetone to extract at room temperature for 3 hours, filter the extract, and repeat the extraction of the residue twice; combine the three extracts, concentrate under reduced pressure to remove most of the solvent, and obtain 25mL of concentrate. Add 100g of silica gel for chromatography into the concentrated solution, stir evenly, so that the hop polyphenol extract is evenly adsorbed on the silica gel of the adsorbent, place it, and volatilize a small amount of solvent therein. Take 300mL of 40% methanol aqueous solution to elute the silica gel adsorbed with hop polyphenol extract, filter, separate the methanol aqueous solution for later use; then use 60-65% methanol aqueous solution 500mL to elute the silica gel, filter and collect the obtained methanol aqueous solution, Add 100 mL of deionized water to the aqueous methanol solution under stirring, and let it stand still. A yellow precipitate precipitates out of the solution,...

Embodiment 3

[0028]Take 400g of hop waste residue, add 1000mL of 95% edible ethanol and ultrasonically extract at room temperature for 1 hour, filter the extract, and repeat the extraction of the residue twice; combine the three extracts, concentrate under reduced pressure to remove most of the solvent, and obtain a 75mL concentrate. Add 280g of diatomaceous earth to the concentrated solution, stir evenly, make the hop polyphenol extract evenly adsorb on the adsorbent diatomite, place it, and volatilize a small amount of solvent therein. Take 500mL of 20% methanol aqueous solution to elute the diatomaceous earth adsorbed with hop polyphenol extract, filter, collect the methanol aqueous solution, and concentrate the filtrate to obtain extract I; then use 30% methanol aqueous solution 500mL to elute the diatomite, filter , collect the obtained aqueous methanol solution, and then concentrate the filtrate to obtain extract II; then use 500 mL of 40% methanol aqueous solution to elute diatomaceo...

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Abstract

The invention provides a method utilizing hop residues to extract xanthohumol. The method includes the following steps: after a hop extractum is extracted by liquid and subcritical or supercritical CO2, hop raffinate (the hop residues) wasted is utilized to extract total polyphenols in hops; a total polyphenol extract is absorbed by an absorbent; then, gradient elution is carried out on the absorbent by an alcohol-water system so as to obtain hop polyphenols of different kinds; meanwhile, the collected xanthohumol is separated and purified to obtain the high-purity one.

Description

technical field [0001] The invention relates to a method for extracting hop polyphenol compounds by using hop waste residue. More specifically, the present invention relates to a method for utilizing liquid, subcritical or supercritical CO 2 The invention discloses a method for extracting, separating and purifying xanthohumol from waste hop raffinate (hop waste residue) after extracting hop extract. Background technique [0002] Hops are one of the important raw materials for beer brewing. Polyphenols in hops account for about 2-10% of the total, including phenolic acids, flavonols, catechins and proanthocyanidins and other four categories, the most content is catechin compounds, including a small amount of epicatechin, gallocatechin, epigallocatechin and so on. The proanthocyanidins in hop polyphenols are mainly proanthocyanidins formed by the condensation of two or more flavan-3-ols, as well as some white anthocyanins, anthocyanins and delphinidins. These compounds Or e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C49/84C07C45/80C07C45/79
CPCY02P20/54
Inventor 刘玉英
Owner 刘玉英
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