Capillary electrophoresis coaxial type magnetic permeance detecting method and device thereof
A technology of capillary electrophoresis and detection device, which is applied in the field of analysis and detection technology and related devices, can solve the problems such as no reports of capillary electrophoresis magnetic permeability detection method and magnetic permeability detector, few measurement objects, low sensitivity, etc., and achieves low cost , easy to use, good versatility
Inactive Publication Date: 2010-07-07
SUN YAT SEN UNIV
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Problems solved by technology
Among them, ultraviolet absorption detection and conductivity detection are more common, but the sensitivity is low; laser-induced fluorescence detection and amperometric detection are high in sensitivity, but there are not many measurement objects; mass spectrometry involves expensive instruments, and the cost is very high
[0004] There is no report about capillary electrophoresis magnetic permeability detection method and magnetic permeability detector
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[0015] Some examples are listed below.
[0016] Fig. 1 is a device for coaxial magnetic permeability detection of capillary electrophoresis. The capillary (1) has an inner diameter of 0.1 cm, an outer diameter of 0.36 cm, and a length of 70 cm, serving as a separation channel. A coil (3) is formed by winding 300-1200 turns of Φ0.17 enameled wire in the region of 8.0-8.2 cm near the end of the capillary, and the width of the coil is 0.1-0.2 cm. A magnetic ring (2) with an inner diameter of 1.2-2.0 cm is placed outside the coil to form a magnetic inductor, and the magnetic circuit direction is coaxial with the capillary separation channel. In order to measure the magnitude of the signal passing through the induction coil, the coil (3) is connected in series with a 1-10k resistor (5) and then connected to the AC signal generator (4). Both ends of the resistor (5) are connected to a voltage measuring device (6), and the signal measured by the voltage measuring device (6) is ampl...
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Abstract
The invention relates to an analyzing and detecting technology and a device thereof. The position of the tail end of a capillary separating channel or the position close to the same is taken as a detecting area; the detecting area is provided with a coaxial magnetic induction coil; and a magnetic circuit of the magnetic induction coil is coaxial to the capillary separating channel. The induction coil is connected with an alternating-current signal generator so as to measure obtained signals of the induction coil, and is connected with a recording instrument after signal amplification. The invention has the advantages of better universality, uneasy pollution, simpler and more convenient application and the like.
Description
technical field [0001] The invention relates to an analysis and detection technology and a related device. In particular, it relates to a detection method of capillary electrophoresis and a corresponding device thereof. Background technique [0002] Capillary electrophoresis is electrophoresis performed in a capillary with high heat dissipation efficiency. It can use high voltage to significantly improve the separation effect. It has the characteristics of high efficiency, low consumption, fast speed and wide application. [0003] Detection technology is one of the core technologies in the capillary electrophoresis analysis method, which directly affects the detection object, detection sensitivity, linear range and instrument cost. The current detection techniques mainly include ultraviolet absorption method, laser induced fluorescence method, conductometric method, amperometric method, mass spectrometry and so on. Among them, ultraviolet absorption detection and conductiv...
Claims
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IPC IPC(8): G01N27/447
Inventor 陈缵光刘翠李偶连杨秀娟司徒贺宪
Owner SUN YAT SEN UNIV