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Enzyme preparation using nicotine dehydrogenase to assist tobacco processing, and preparation and use methods

A nicotine dehydrogenase enzyme and a nicotine dehydrogenase technology are applied in the field of enzyme preparations for improving tobacco grades, can solve the problems of low nicotine content and affect the results of smoking evaluation of tobacco leaves, etc., and achieve the reduction of nicotine content and ease of use. Effect

Active Publication Date: 2010-07-14
云南万芳生物技术有限公司
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

However, if the nicotine content is too low, the difference in the ratio of total nitrogen / nicotine and reducing sugar / nicotine will affect the results of tobacco leaf smoking evaluation

Method used

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  • Enzyme preparation using nicotine dehydrogenase to assist tobacco processing, and preparation and use methods

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Experimental program
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Effect test

Embodiment Construction

[0041] (1) Preparation and determination of the preparation of the present invention

[0042] Nicotine dehydrogenase can be purchased directly from microbial fermentation products. Or use the corresponding nicotine dehydrogenase-producing strains to ferment and produce crude products through cell wall breaking, release and preliminary extraction. Then, refine, remove metal ions, dry and concentrate with the method of the present invention to obtain the nicotine dehydrogenation preparation.

[0043] Dissolve and dilute the solid nicotine dehydrogenase crude product with W / V=1g:20mL or liquid W / V=1mL:10mL buffer, the pH value of Britton-Robinsion buffer is 7.0; centrifuge at 3500r / min for 20min, take the Clear solution, add (NH 4 ) 2 SO 4 , so that the final concentration of ammonium sulfate is 62%, let it stand for 24h, then centrifuge at 15000r / min at 4°C for 30min, take the supernatant, and add (NH 4 ) 2 SO 4 Overnight, centrifuge again at 15000r / min ...

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PUM

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Abstract

The invention relates to an enzyme preparation using nicotine dehydrogenase to assist tobacco processing, and preparation and use methods, and belongs to an enzyme preparation which is added before tobacco redrying to improve the grade of tobacco. The invention carries out impurity removal and refining for the known nicotine dehydrogenase source, and uses an EDTA complexing agent to remove metal ions in an enzyme solution, so that the enzyme in a condensed original solution shows the enzyme activity and stably exists in a passivation form favorable for storing for a longer time, and is condensed and prepared into a tobacco active additive by the freeze drying method. When in use, water is used to dilute so as to replace original second water for moistening when tobacco strips are redried, so that the nicotine content of tobacco can be better degraded, and the smoking safety of tobacco can be improved.

Description

technical field [0001] The invention belongs to an enzyme preparation for improving the grade of tobacco added before rebaking of tobacco. Background technique [0002] Microorganisms and enzymes play very important roles during tobacco leaf growth, conditioning, aging, processing and storage. In the mid-1850s, people began to use microorganisms or enzymes to improve the quality of tobacco leaves, tobacco stems and tobacco flakes, produce spices and degrade nicotine, which is still a hot research topic. Nicotine is an alkaloid in common tobacco, commonly known as nicotine (Nicotine), which acts on autonomic nerves, central nervous system and motor nerve endings. The content is directly proportional. With the continuous deepening of the worldwide anti-smoking campaign and consumers paying more attention to their own health, almost all types of cigarette products are developing in the direction of low-tar and low-nicotine light-flavored cigarettes. There are many upper toba...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/02C12Q1/32A24B15/20
Inventor 韩伟何宝玉陈应昆
Owner 云南万芳生物技术有限公司
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