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Primer evaluation method, primer evaluation program, and real-time polymerase chain reaction apparatus

A time-varying and incremental technology, applied in biochemical cleaning devices, biochemical equipment and methods, enzymology/microbiology devices, etc., can solve problems such as reducing the comparative value of quantified nucleic acids

Inactive Publication Date: 2012-11-28
SONY CORP
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Problems solved by technology

Therefore, for example, in the case of monitoring the amount of nucleic acid in a certain active substance every predetermined period of time, the amplification efficiency of nucleic acid changes due to the difference between the temperatures at the respective quantification timings, thus reducing the quantification of nucleic acid. comparative value of

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  • Primer evaluation method, primer evaluation program, and real-time polymerase chain reaction apparatus
  • Primer evaluation method, primer evaluation program, and real-time polymerase chain reaction apparatus
  • Primer evaluation method, primer evaluation program, and real-time polymerase chain reaction apparatus

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Embodiment Construction

[0025] The mode of carrying out the present invention will be described below. The description will be performed in the following order.

[0026]

[0027] [1-1. Configuration of real-time PCR apparatus]

[0028] [1-2. Configuration of primer evaluation index submitter]

[0029] [1-3. Process of primer evaluation index submission processing]

[0030] [1-4. Advantageous effects, etc.]

[0031]

[0032]

[0033] [1-1. Configuration of real-time PCR apparatus]

[0034] exist figure 1 , a schematic configuration of the real-time PCR apparatus 1 is shown. This real-time PCR apparatus 1 has a structure obtained by layering a plurality of substrates 11 to 16 at predetermined intervals for the reaction chamber RM.

[0035] The reaction substrate 11 is a substrate serving as a base layer. In this substrate, containers (hereinafter also referred to as wells) UL each serving as an amplification reaction site for nucleic acid are formed at high density. To these wells UL, tar...

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Abstract

A primer evaluation method includes: acquiring a signal indicating time change in an amplification amount obtained when sample sets prepared for the number of temperature conditions that should be made different from each other at an annealing stage in units of target nucleic acids diluted in a stepwise manner are so amplified that a temperature condition at a stage other than the annealing stageis fixed; acquiring a signal indicating initial amounts of the target nucleic acids diluted in a stepwise manner; obtaining amplification efficiency for each of the temperature conditions based on the time change in the amplification amount and the initial amount, and calculating a variation degree of the amplification efficiency; and submitting the variation degree and a reference value for quality evaluation of a primer, set with respect to the variation degree.

Description

technical field [0001] The present invention relates to a primer evaluation method, a primer evaluation program, and a real-time polymerase chain reaction PCR device, and is applicable, for example, in the technical field of amplifying nucleic acid. Background technique [0002] In the polymerase chain reaction (PCR), deoxyribonucleic acid (DNA), which is the subject of amplification, DNA polymerase, and a large number of primers are mixed together. Primers are short nucleic acid segments that act to provide DNA polymerase with a starting point for the DNA synthesis reaction and are essential in any DNA synthesis reaction. Therefore, the design of primers is very important. [0003] For the design of primers, a method of synthesizing and screening a plurality of sequences that should be used as candidates to obtain an optimal sequence satisfying predetermined conditions has been proposed (refer to, for example, Published Japanese Patent No. 2003-210175 and Published Japanes...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G06F19/00C12M1/36C12M1/00C12N15/09C12Q1/68
CPCB01L7/52B01L2300/0654B01L2300/1822B01L2300/0819C12Q1/6851B01L2200/147B01L7/54C12Q2527/101
Inventor 阿部友照中川和博田中里实
Owner SONY CORP
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