Method for extracting dihydromyricetin from vine tea

A technology of dihydromyricetin and rattan tea, applied in the direction of organic chemistry, etc., to achieve the effects of saving production costs, convenient operation, and large output

Inactive Publication Date: 2010-08-18
安徽省瑞森生物科技有限责任公司
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AI-Extracted Technical Summary

Problems solved by technology

However, the research on the development, utilization and clini...
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Abstract

The invention discloses a method for extracting dihydromyricetin from vine tea, including the following steps: (1) vine tea dry leaves are weighed, subject to microwave radiation treatment by a microwave oven and then smashed; (2) the particles are added into a microwave reverse flow extractor, water is added, microwave extraction and filtering are carried out, thus obtaining filtration A and residue A; (3) the residue A is processed according to the method in the step (2), thus obtaining filtration B and residue B; (4) residue A and residue B are merged and added into the microwave reverse flow extractor, ethanol is added, microwave extraction is carried out for 30-60min and then filtering is carried out, thus obtaining filtration C; (5) the filtrations A, B and C are merged, and then concentration, crystallization and drying are carried out. The invention has simple technology and convenient operation, extraction ratio of dihydromyricetin can reach 47.3%, purity can reach 85%, the required organic solvent is less, operating safety is good, no high temperature furnace is required for heating, energy consumption is reduced, and production cost is saved.

Application Domain

Organic chemistry

Technology Topic

ChemistryOrganic solvent +11

Examples

  • Experimental program(3)

Example Embodiment

[0019] Example 1
[0020] (1) Weigh 10 kg of dried leaves of rattan tea, use a microwave oven to conduct microwave radiation treatment at a microwave power of 900w, and crush them to 80-100 mesh particles;
[0021] (2) Add the above particles to the WNT-B microwave countercurrent extraction equipment, and then add 100kg of water, extract for 60 minutes at a microwave power of 1000w, and filter to obtain filtrate A and filter residue A;
[0022] (3) Add filter residue A to the WNT-B microwave countercurrent extraction equipment, add 100kg of water, extract for 60 minutes at a microwave power of 1000w, and filter to obtain filtrate B and filter residue B;
[0023] (4) Combine filter residue A and filter residue B with a total of 5.3 kg, add them to the microwave countercurrent extraction equipment, and then add 15 kg of ethanol with a mass fraction of 70%, extract for 50 minutes at a microwave power of 1000w, and filter to obtain filtrate C;
[0024] (5) Combine filtrate A, filtrate B and filtrate C, concentrate, crystallize, and dry to obtain pure dihydromyricetin with an extraction rate of 43.6% and a purity of 83.6%.

Example Embodiment

[0025] Example 2
[0026] (1) Weigh 10 kg of dry leaves of rattan tea, use a microwave oven to conduct microwave radiation treatment at a microwave power of 800w, and crush them to 80-100 mesh particles;
[0027] (2) Add the above particles to the WNT-B microwave countercurrent extraction equipment, and then add 60kg of water, extract for 30 minutes at a microwave power of 1500w, and filter to obtain filtrate A and filter residue A;
[0028] (3) Add filter residue A to the WNT-B microwave countercurrent extraction equipment, add 60kg of water, extract for 30 minutes at a microwave power of 1500w, and filter to obtain filtrate B and filter residue B;
[0029] (4) Combine the filter residue A and the filter residue B with a total of 5.1 kg, add them to the microwave countercurrent extraction equipment, and then add 12 kg of 70% ethanol with a microwave power of 1500w for 30 minutes and filter to obtain filtrate C;
[0030] (5) Combine filtrate A, filtrate B and filtrate C, concentrate, crystallize, and dry to obtain pure dihydromyricetin with an extraction rate of 47.3% and a purity of 85.0%.

Example Embodiment

[0031] Example 3
[0032] (1) Weigh 10 kg of dry leaves of rattan tea, use a microwave oven to conduct microwave radiation treatment at a microwave power of 800w, and crush them to 80-100 mesh particles;
[0033] (2) Add the above particles to the WNT-B type microwave countercurrent extraction equipment, then add 120kg of water, extract for 90 minutes at a microwave power of 1200w, and filter to obtain filtrate A and filter residue A;
[0034] (3) Add filter residue A to WNT-B type microwave countercurrent extraction equipment, add 120kg of water, extract for 90 minutes at a microwave power of 1200w, and filter to obtain filtrate B and filter residue B;
[0035] (4) Combine filter residue A and filter residue B with a total of 5.5 kg, add them to the microwave countercurrent extraction equipment, add 18 kg of ethanol with a mass fraction of 50%, extract for 30 minutes at a microwave power of 1200w, and filter to obtain filtrate C;
[0036] (5) Combine filtrate A, filtrate B and filtrate C, concentrate, crystallize, and dry to obtain pure dihydromyricetin with an extraction rate of 45.7% and a purity of 81.3%.
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Description & Claims & Application Information

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Classification and recommendation of technical efficacy words

  • Reduce extraction time
  • Improve efficiency
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