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Kit for detecting alpha 1-microglobulin by nano microsphere immunonephelometry

A microglobulin and nano-microsphere technology, which is applied in the field of α1-microglobulin kits, can solve the problems of inability to perform batch sample analysis, undetectable, cumbersome operations, etc., and achieve strong anti-interference ability, simple operation, and accuracy Good results

Active Publication Date: 2010-09-01
浙江伊利康生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Known Determination α 1 - Microglobulin (α 1 -MG) methods include immunodiffusion, immunoelectrophoresis, radioimmunoassay, and enzyme-linked immunoassay (ELISA). These methods are cumbersome to operate and require special equipment. Samples need to be pretreated and cannot be analyzed in batches. and can not be directly used for automatic biochemical analyzer detection and other shortcomings

Method used

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  • Kit for detecting alpha 1-microglobulin by nano microsphere immunonephelometry
  • Kit for detecting alpha 1-microglobulin by nano microsphere immunonephelometry
  • Kit for detecting alpha 1-microglobulin by nano microsphere immunonephelometry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1. α 1 -MG solution (reagent R 1 )

[0032] Tris (buffer) 50mmol / L

[0033] Tween-20 (surfactant) 0.5mmol / L

[0034] NaCl (electrolyte) 100mmol / L

[0035] PEG-6000 (reaction accelerator) 4mmol / L

[0036] Broad-spectrum fungicide (preservative) 3mmol / L

[0037] Disodium EDTA (stabilizer) 5mmol / L

[0038] The rest is purified water

[0039] 2. Anti-human alpha 1 -MG antibody solution (reagent R 2 )

[0040]Dilute the goat anti-human α1-MG antibody polyclonal antibody and 150nm nanospheres (the weight ratio of antibody and microspheres is 1:1) at room temperature with 100mmol / L Tris buffer (pH 7.0). After the two are mixed, shake and adsorb at room temperature for 2 hours (i.e., physical adsorption method known to those skilled in the art), add 0.1% BSA-containing trishydroxymethylaminotetrane buffer to block for 1 hour, centrifuge to remove the supernatant, and use 100 mmol / L tris buffer solution is diluted to concentration and is 0.3%, and adds preservative; ...

Embodiment 2

[0051] 1. α 1 -MG solution (reagent R 1 )

[0052] Tris (buffer) 100mmol / L

[0053] Tween-20 (surfactant) 4mmol / L

[0054] NaCl (electrolyte) 60mmol / L

[0055] PEG-6000 (reaction accelerator) 8mmol / L

[0056] Broad-spectrum fungicide (preservative) 0.1mmol / L

[0057] Disodium EDTA (stabilizer) 0.5mmol / L

[0058] The rest is purified water

[0059] 2. Anti-human alpha 1 -MG antibody solution (reagent R 2 )

[0060] Dilute goat anti-human alpha with 150mmol / L Tris buffer (pH7.4) at room temperature 1 -MG antibody polyclonal antibody and 50nm nanometer microspheres (the weight ratio of antibody and microsphere is 1: 1), after mixing the two, shake and adsorb at room temperature for 2 hours (i.e. the physical adsorption method known to those skilled in the art), Add tris buffer containing 0.1% BSA to block for 1 hour, centrifuge to remove supernatant, dilute to 4% concentration with 100mmol / L tris buffer, and add appropriate technology in the art Preservatives known to...

Embodiment 3

[0071] 1. α 1 -MG solution (reagent R 1 )

[0072] Tris (buffer) 200mmol / L

[0073] Tween-20 (surfactant) 2mmol / L

[0074] NaCl (electrolyte) 200mmol / L

[0075] PEG-6000 (reaction accelerator) 5mmol / L

[0076] Broad-spectrum fungicide (preservative) 3mmol / L

[0077] Disodium EDTA (stabilizer) 2mmol / L

[0078] The rest is purified water

[0079] 2. Anti-human alpha 1 -MG antibody solution (reagent R 2 )

[0080] Dilute the goat anti-human α1-MG antibody polyclonal antibody and 100nm nanospheres (the weight ratio of antibody and microspheres is 1:1) at room temperature with 200mmol / L Tris buffer (pH 7.4). After the two are mixed, shake and adsorb at room temperature for 2 hours (i.e., physical adsorption method known to those skilled in the art), add 0.1% BSA-containing trishydroxymethylaminotetrane buffer to block for 1 hour, centrifuge to remove the supernatant, and use 100 mmol / L Tris buffer solution was diluted to a concentration of 2%, and an appropriate preserv...

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Abstract

The invention relates to a kit for detecting alpha 1-microglobulin in blood serum. In order to solve technical problems, a reagent provided by the invention has the characteristics of no need of dilution of a sample, simple operation, high precision, good repeatability and suitability for various full-automatic biochemical analyzers. The invention adopts a technical scheme that the kit for detecting the alphal-microglobulin by nano microsphere immunonephelometry comprises the following components: a, a reagent R1 which comprises buffer solution, a stabilizing agent, an electrolyte, a surfactant and a proper amount of preservative; b, a reagent R2 which comprises buffer solution, an antihuman alpha 1-MG antibody polyclonal antibody and nano microspheres and a proper amount of preservative;and c, a reference calibration material which comprises buffer solution, a stabilizing agent, a proper amount of preservative and recombinant alpha 1-microglobulin pure product of which the mount is determined according to the needs of concentration.

Description

technical field [0001] The invention relates to a kit for measuring serum components, in particular to a kit for measuring α1-microglobulin (α1-MG) in serum, which can be widely used in the technical fields of medicine and biochemistry. Background technique [0002] alpha 1- - Microglobulin (α 1 -MG) is mainly synthesized in liver and lymphocyte tissue, widely distributed in body fluids and a glycoprotein on the surface of lymphocyte membrane, with a molecular weight of 27KD, α in blood 1 - Microglobulin has various forms such as free and bound to IgA; under normal circumstances, free α in the blood 1 - Microglobulins are freely filtered through the glomerulus and reabsorbed in the proximal convoluted tubule, so urine levels are minimal. α 1 - Microglobulins cannot be filtered by the glomerulus. due to alpha 1 - The production of microglobulin is constant and less affected by extrarenal factors, so the determination of α 1 - Microglobulin concentration has early diagn...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/531G01N33/539G01N21/31
Inventor 王贤理蒙凯蔡其浩
Owner 浙江伊利康生物技术有限公司
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