Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for separating alpha s-casein

A separation method and casein technology, which can be applied to the preparation methods of peptides, chemical instruments and methods, animal/human peptides, etc., can solve the problems of cumbersome steps, long time consumption, etc., and achieve good application prospects, simple and easy operation, and effective The effect that is beneficial to development and utilization

Active Publication Date: 2012-11-07
宁夏塞尚乳业有限公司
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The steps of this method are cumbersome. After two times of dissolution, precipitation and water washing, it takes a long time, the total time is about 4 hours, the yield of α-casein is 69.5%, the purity is only 74.2%, and the yield of β-casein is 26.5%. , the purity is only 75.0%

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for separating alpha s-casein
  • Method for separating alpha s-casein
  • Method for separating alpha s-casein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) Weigh 6.75g of casein and dissolve it in water at 30°C, keep it at 30°C for 10 minutes to obtain an aqueous solution of casein with a mass-volume percentage concentration of 2.7%, adjust the pH value to 11.0, and let the solution stand for 3 minutes;

[0022] (2) Add CaCl to casein aqueous solution 2 solution to Ca 2+ When the concentration reaches 0.05mol / L, adjust the pH value to 7.0, centrifuge at 3000rmp for 10min, and collect the precipitate;

[0023] (3) Dissolve the precipitate in 6.6mol / L urea aqueous solution, and the solution contains 1.8mol / L NaCl, then add distilled water to dilute the urea concentration to 3.3mol / L, adjust the pH value to 4.6, centrifuge at 3000rmp for 10min, respectively The supernatant and the precipitate were collected, and the precipitate was washed twice with water, then freeze-dried to obtain α s - casein;

[0024] (4) Add distilled water to the supernatant in step (3), so that the urea concentration in the solution reaches 1.7...

Embodiment 2

[0032] (1) Weigh 6.75g of casein and dissolve it in water at 30°C, keep it at 30°C for 10 minutes to obtain a casein aqueous solution with a mass-volume percentage concentration of 2.7%, then adjust the pH value to 11.0, and let the solution stand for 1 minute;

[0033] (2) Add CaCl to casein aqueous solution 2 solution to Ca 2+ When the concentration reaches 0.04mol / L, adjust the pH value to 7.0, centrifuge at 3000rmp for 10min, and collect the precipitate;

[0034] (3) Dissolve the precipitate in 6.6mol / L urea aqueous solution, and the solution contains 1.8mol / L NaCl, then add distilled water to dilute the urea concentration to 3.3mol / L, adjust the pH value to 4.6, centrifuge at 3000rmp for 10min, respectively The supernatant and the precipitate were collected, and the precipitate was washed 3 times with water, then freeze-dried to obtain α s - casein;

[0035] (4) Add distilled water to the supernatant in step (3), so that the urea concentration in the solution reaches 1...

Embodiment 3

[0038] (1) Weigh 6.75g of casein and dissolve it in water at 30°C, keep it at 30°C for 10 minutes to obtain an aqueous solution of casein with a mass-volume percentage concentration of 2.7%, then adjust the pH value to 11.0, and let the solution stand for 4 minutes;

[0039] (2) Add CaCl to casein aqueous solution 2 solution to Ca 2+ When the concentration reaches 0.06mol / L, adjust the pH value to 7.0, centrifuge at 3000rmp for 10min, and collect the precipitate;

[0040] (3) Dissolve the precipitate in 6.6mol / L urea aqueous solution, and the solution contains 1.8mol / L NaCl, then add distilled water to dilute the urea concentration to 3.3mol / L, adjust the pH value to 4.6, centrifuge at 3000rmp for 10min, respectively The supernatant and the precipitate were collected, and the precipitate was washed twice with water, then freeze-dried to obtain α s - casein;

[0041] (4) Add distilled water to the supernatant in step (3), so that the urea concentration in the solution reache...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for separating alpha s-casein, which belongs to the technical field of casein separation. The method comprises the following steps of: separating milk casein, which is used as a raw material, by a chemical fractionation method; separating Kappa -casein from alpha s-casein and beta-casein according to the characteristic that the Kappa -casein is non-Ca2+ sensitive casein and can be dissolved into calcium ion-containing solution; and according to the characteristic that the dissolvability of the beta-casein and the alpha-casein in urea in solution of urea with different concentration is different, separating the beta-casein from the alpha-casein by adjusting the concentration of urea in the solution. The method has the advantages that: the operation is simple and practicable; only the conventional equipment is needed; the time of the method is about 2 to 3 hours; the recovery ratio of the obtained alpha s-casein component is high and the purity can reach97.8 percent; and through the deep processing of the milk casein, the corresponding milk bioactive peptide can be obtained by hydrolyzing the obtained alpha s-casein, so that the ratio of the target active peptide in the product is greatly increased.

Description

technical field [0001] The invention belongs to casein separation technology, in particular to a s - Isolation method of casein. In this method, milk casein is used as raw material, and the main component α is separated by chemical fractionation. s - Casein separation, while effectively separating the β-casein fraction. Background technique [0002] Casein (Cn), also known as casein, includes α s1 - casein, alpha s2 - Casein, β-casein (β-Cn) and κ-casein (κ-Cn), where α s1 - casein and alpha s2 -Caseins are collectively referred to as alpha s - Casein (α s -Cn), α s -Cn accounts for about 47% of the total casein. It is an important source of milk-derived bioactive peptides. alpha s -The rich phosphate group content in casein makes it the best raw material for preparing casein phosphopeptide (CPP) which promotes calcium absorption in human body. alpha s - Casein products have good foamability and can be used as food additives in food production to improve the pro...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/47C07K1/30
Inventor 张列兵刘晓辉吴方旭李妍
Owner 宁夏塞尚乳业有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com