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Method for preparing rice into glucose for injection

A technology for glucose and injection, applied in the field of biopharmaceuticals, can solve the problems of many impurities and difficulties in glucose for injection, and achieve the effect of improving the conversion rate, increasing the high-tech content, and increasing the added value.

Inactive Publication Date: 2010-09-15
WANFU BIOTECHNOLOGY (HUNAN) AGRI DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The DE of corn starch after liquefaction and saccharification can reach more than 98%, while the liquefaction and saccharification of rice starch is difficult to complete, and there are many impurities, so it is very difficult to make glucose for injection

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0014] Wash the rice directly with water and soak for 4 hours, crush it into 60 mesh, add distilled water according to the ratio of material to liquid 1:5, preheat to 95°C, add CaCl according to 0.3% of the weight of the rice 2 , adjust the pH to 6.2; add high-temperature a-amylase 60U / g, stir while adding, keep the temperature at 95°C, and the reaction time is 40min. At this moment, the iodine addition reaction was colorless, and the DE (glucose value) value was 20, and it was centrifuged at 1000r / min.

[0015] After liquefaction, the tank pressure is 1.4150MPa, the temperature is raised to 110°C, and the enzyme is killed for 10 minutes.

[0016] Saccharification: After adjusting the pH value to 4.8 with HCL, add 120 U / g of glucoamylase and 0.25 apu / g of pullulanase. Saccharify for 48 hours at a tank pressure of 0.3 MPa at a temperature of 60°C. No reaction is found at the end of iodine addition.

[0017] Neutralization and filtration: cool the saccharification solution to b...

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Abstract

The invention relates to a method for preparing rice into glucose for injection. The method comprises the following steps of: removing protein in rice by adopting amylase; then, hydrolyzing and saccharifying the protein of broken rice separately with alpha-amylase, diastatic enzyme and pullulanase; decoloring, removing impurities and filtering by using active carbon; removing ions by using ion exchange columns; crystallizing and drying; and finally obtaining qualified glucose powder for injection. The invention has the advantages of improving the starch conversion rate and the added values and the high-tech contents of rice processing, reducing the production cost for preparing starch into glucose for injection, changing waste into valuable, and promoting the development of rice-processing industrialization.

Description

technical field [0001] The invention provides a method for preparing glucose for injection from rice, which belongs to the technical field of biopharmaceuticals. Background technique [0002] Currently used glucose for injection is prepared from cornstarch, Japan uses cassava to prepare glucose for injection, rice (broken rice) starch has only been made into edible glucose, and there is no report of making glucose for injection. Corn contains less protein and has a large starch particle size. Broken rice contains 7.0-10.0% protein, the starch particle size is small and the starch and protein are combined into a tight network structure. The size of starch granules determines the gelatinization temperature of starch and affects the liquefaction of starch. Corn starch has a low gelatinization temperature and is easy to liquefy, while rice starch has a small particle size and a high gelatinization temperature of 85°C, and is tightly combined with protein and is not easy to liq...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14
Inventor 龚永福贺福元叶华杨志荣杨晓华王湛淅刘文龙石继连邹欢
Owner WANFU BIOTECHNOLOGY (HUNAN) AGRI DEV CO LTD