Codling moth identification primer, identification method and application

A technology of codling moth and primer pair, which is applied to distinguish codling moth, small pear borer and plum small borer, in the field of peach small borer, which can solve the problems of increasing the work intensity of quarantine personnel and prolonging the customs clearance time of import and export items, etc. , to achieve the effects of shortening customs clearance time, good amplification effect, and high success rate

Inactive Publication Date: 2012-09-26
NORTHWEST A & F UNIV
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  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

However, in the actual quarantine work at the port, it is often the young larvae or eggs that are intercepted. The usual practice is to raise them indoors and wait for the mature larvae to be identified. This not only prolongs the customs clearance time for import and export items, but also increases Work intensity of quarantine personnel

Method used

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  • Codling moth identification primer, identification method and application
  • Codling moth identification primer, identification method and application
  • Codling moth identification primer, identification method and application

Examples

Experimental program
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Effect test

Embodiment

[0083] Specific codling moth identification primers IF / R were used to rapidly identify samples of codling moth, small peach borer, pear borer and plum borer collected from different regions in China.

[0084] Primer 1F: 5′-ATTACCCCCATCTATTATACTT-3′

[0085] Primer 1R: 5′-TGGTAATGATAAAAGAAGTAAAAAGAGC-3′

[0086] Step 1: Extraction of total DNA from borer worms to be identified

[0087] 1. Specimen collection information: 10 codling moth samples, 5 peach borer samples, 4 pear borer samples and 3 plum borer samples were collected from different regions in my country.

[0088]

[0089] 2. Extraction of DNA template

[0090] Randomly select a single borer specimen (preserved in pure alcohol), take the head from the larvae, and take the legs or chest muscles from the adult; after taking out the sample, immerse it in sterile water to remove the alcohol, and dry it on absorbent paper; transfer the sample to 100 μL for extraction after drying Grind thoroughly in the liquid; move ...

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Abstract

The invention relates to a codling moth identification primer, which comprises a primer 1F: 5'-ATTACCCCCATCTATTATACTT-3' and a primer 1R: 5'-TGGTAATGATAAAAGAAGTAAAAGAGC-3'. A method for identifying codling moths by use of the primer comprises the following steps: (1) extracting a DNA template of an identification sample; (2) using the codling moth identification primer in right claim 1 to performPCR amplification on template DNA; and (3) detecting the existence of 289bp band through electrophoresis. The codling moth identification primer provided by the invention has good amplification effect on target fragments of codling moths, can identify samples only according to the existence of the 289bp band when the primer is used for identification, and has the advantages of quickness, convenience, clear results and accuracy.

Description

technical field [0001] The present invention relates to a codling moth identification primer and a method for quickly identifying and identifying codling moth by using the specific identification primer to identify a sample to be identified. Application on pear borer and plum borer. Background technique [0002] The codling moth Cydia pomonella L., synonymous with Laspeyresia Pomonolla L., commonly known as the small apple moth, belongs to Lepidoptera, Toriidae, mainly harms apples, pears, sago, crabapple, plum, peach, hawthorn and other fruit trees , is a devastating pest of pome fruit and drupe fruit trees, and is a worldwide quarantine pest. The larvae damage the fruit with larvae, causing a large number of insects and fruit, seriously affecting the quality of the fruit; the larvae also have the habit of turning fruit, causing a large number of fruit drop and rot before the fruit is harvested. The fruit-eating rate of the larvae is usually about 50%. It can reach 70%-10...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/11
Inventor 冯纪年董昆杨建强赵骁
Owner NORTHWEST A & F UNIV
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