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Method for testing efficacy of swine fever live vaccine

A swine fever live vaccine and inspection method technology, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, measuring devices, etc. Uniformity, significant differences in experimental results, and low cost

Active Publication Date: 2010-09-29
PU LIKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

Not only does it require a lot of experiments with white rabbits, which is time-consuming and laborious, but also because the number of pure white rabbits in my country is gradually decreasing and the strains are becoming more and more complex, it is difficult to accurately detect the efficacy of live vaccines by using rabbit body reaction heat

Method used

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  • Method for testing efficacy of swine fever live vaccine
  • Method for testing efficacy of swine fever live vaccine

Examples

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Embodiment 1

[0031] Test method for efficacy of swine fever live vaccine

[0032] 1. Preparation of Cells for Passaging

[0033] The porcine testis (ST) cells (derived from ATCC, American Type Culture Collection) covered with a single layer were digested and dispersed with 0.25% trypsin (containing 0.02% EDTA) and then added with 3% fetal bovine serum α-MEM cells were maintained in culture medium, and after cell counting, the cell suspension was added to a 96-well cell plate, 100 μl per well.

[0034] 2. Dilution of live swine fever vaccine

[0035] In this example, three batches of live vaccines were prepared, and their antigen contents were determined respectively.

[0036] The strain of the swine fever live vaccine is an attenuated strain of swine fever lathe (preserved by China Veterinary Drug Administration, preservation number AV1412). Inoculate ST cells with attenuated CSF rabbitized strain, and mix the harvested virus antigen solution with appropriate lyoprotectant to make CSF l...

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Abstract

The invention belongs to the technical field of new biological veterinary medicaments, and in particular relates to a method for testing the efficacy of a swine fever live vaccine. The efficacy of the live vaccine is evaluated by detecting the virus content of the live vaccine through indirect immunofluorescence (IFA). The method for testing the efficacy of the swine fever live vaccine has the advantages of short test time, simple operation, low cost, accurate obtained result, high repeatability, excellent application value and wide market prospects.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for testing the efficacy of a swine fever live vaccine. Background technique [0002] Classical Swine fever (CSF for short), also known as Hog cholera or European swine fever, is a viral infectious disease with acute or hyperacute fever in pigs, clinically characterized by dissemination Rapid, febrile, typical hemorrhagic lesions can be seen on autopsy. Swine fever can infect pigs of all ages and is prevalent throughout the year with high morbidity and mortality, which is extremely harmful to pigs. This disease is one of the most important infectious diseases threatening the pig industry. The World Organization for Animal Health (OIE) lists swine fever as one of the 16 notifiable infectious diseases of category A, and China defines it as a severe infectious disease. Mandatory immunization with swine fever live vaccines is currently the best way to prevent swine fever in m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N21/64C12Q1/02
Inventor 张许科孙进忠乔荣岑
Owner PU LIKE BIO ENG
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