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In-vitro multicellular line coculture dish and manufacturing method thereof

A production method and co-cultivation technology, applied in tissue cell/virus culture devices, biochemical equipment and methods, biochemical instruments, etc., can solve the problems of inconsistency in culture medium and inconvenient observation of cell growth, and achieve simple structure, The effect of low cost and small investment

Inactive Publication Date: 2010-10-06
程计林 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, both suspension cell co-culture and cell migration have their drawbacks. One is that the medium cannot be unified, and the other is that it is very inconvenient to observe cell growth.

Method used

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  • In-vitro multicellular line coculture dish and manufacturing method thereof
  • In-vitro multicellular line coculture dish and manufacturing method thereof
  • In-vitro multicellular line coculture dish and manufacturing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] see figure 1 , an in vitro multi-cell line co-culture dish related to the present invention is composed of a housing 1, a diaphragm 2 and a partition 3, the diaphragm 2 is arranged in the housing 1, and the housing 1 is divided into two chambers, A partition 3 is arranged beside the diaphragm 2, and the partition 3 is a detachable partition;

[0014] The diaphragm 2 is made of polycarbonate film;

[0015] The material of the partition 3 is plastic, which is the same as that of the housing 1 .

Embodiment 2

[0017] The difference between this embodiment and Embodiment 1 is that the diaphragm 2 is arranged vertically and horizontally in the casing, and divides the casing 1 into four chambers.

[0018] The in vitro multi-cell line co-culture dish and the manufacturing method thereof of the present invention can be produced into bottles or plates of different sizes and specifications and different compartments.

[0019] During manufacture, the shell is prepared first, and then a polycarbonate diaphragm is arranged inside the shell, and notches are arranged on the bottom and both sides of the shell next to the polycarbonate diaphragm, and partitions are inserted in the notches.

[0020] working principle:

[0021] Two or more different strains of cells are cultured separately with one or more polycarbonate membranes that can only allow cell-secreted factors or molecules to pass freely, and cells cannot pass through. There is a plastic partition next to the polycarbonate membrane, acco...

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Abstract

The invention relates to an in-vitro multicellular line coculture dish and a manufacturing method thereof. The in-vitro multicellular line coculture dish comprises a shell (1). The in-vitro multicellular line coculture dish is characterized by also comprising a diaphragm (2) and a partition (3), wherein the diaphragm (2) is arranged in the shell (1) and divides the shell (1) into a plurality of chambers; the partition (3) is detachable and is arranged beside the diaphragm (2); and the diaphragm (2) is a polycarbonate film. The in-vitro multicellular line coculture dish has simple structure, and can maximally simulate the living environment of complex cells in vivo. By using the in-vitro culture dish, multiple cells, which may interact with each other, can be cultured in the same environment; and the cells do not contact with each other, but cell factors secreted by the cells can interact with each other, thereby simulating an in-vivo physiological environment in vitro and completing the positive and negative feedback effects among the cells. The manufacturing method is simple and has the advantages of low investment and low cost.

Description

(1) Technical field [0001] The invention relates to an in vitro multi-cell line co-culture dish and a manufacturing method thereof, and belongs to the field of cell culture devices in experiments of biomedicine or in biopharmaceuticals. (2) Background technology [0002] Cell culture is an important part of in vitro experiments in medical scientific research. Conventional cell culture is to implant the same kind of cells into a petri dish (bottle or plate), grow the cells in vitro by adding the nutrients needed by the cells, obtain a large number of cells or their metabolites, and then use corresponding technical means to treat them. It detects and analyzes the results to obtain a physiological or pathological phenomenon. Traditional cell culture dishes can culture cells in vitro to test a regulatory factor or result, but complex intercellular regulation in vivo, that is, multi-factor or sequential effects, cannot be fully simulated in vitro. This is because the cell cultu...

Claims

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Application Information

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IPC IPC(8): C12M3/00C12M1/22
CPCC12M23/38C12M21/06C12M47/04
Inventor 程计林程远
Owner 程计林
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