Method for producing coniothyrium minitans conidium

Abstract

The invention provides a method for producing coniothyrium minitans conidium which takes agricultural wastes as raw materials. The method comprises the following steps: (1) pulverizing and drying the agricultural wastes; (2) preparing a matrix mixture; (3) preparing spore suspension; (4) inoculating; and (5) culturing. In the invention, the coniothyrium minitans conidium is prepared from the agricultural wastes; and after measurement, the final spore yield reaches 1.58*109-6.0*109pcs / g matrixes and has no significant difference with the spore yield by single wheat fermentation. The method overcomes the defect of high cost in the prior art, has very low cost and very good production effect, and can make use of the agricultural wastes.

Description

technical field

[0001] The invention relates to a method for producing conidia of shell mold. Background technique

[0002] Shell mold is a biocontrol fungus for controlling Sclerotinia sclerotiorum, which can be used as a high-efficiency biopesticide. The first prerequisite for the application of C. shield in production practice is to obtain a large number of active conidia. Studies have found that C. shieldiae does not produce conidia under liquid fermentation conditions, and produces little or no conidia under semi-solid conditions; using PDA plates for solid culture of C. shieldiae can produce a large number of conidia, but this production The method is inconvenient for industrial expansion and cultivation production. There is another method, which is to use wheat, oats, barley, etc. as substrates to cultivate C. shield, because these substrates contain a large amount of carbon and nitrogen sources, which can make C. shield produce a large number of conidia, and can be...