Process for improving fermentation conversion rate of enzyme preparation

A technology of enzyme preparation and conversion rate, which is applied in the field of biochemistry, can solve problems such as the inability to fully meet the needs of large-scale production of enzyme preparations, and achieve the effects of improving energy utilization, ensuring aerobic demand, and increasing production

Inactive Publication Date: 2010-10-06
江苏博立生物制品有限公司
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Problems solved by technology

However, the above methods still cannot fully meet th

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  • Process for improving fermentation conversion rate of enzyme preparation

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Embodiment Construction

[0012] The present invention will be further described by taking the production of glucoamylase by Aspergillus niger as an example below.

[0013] Slant medium: 30g sucrose, NaNO 3 2g, K 2 HPO 4 1g, KCl 0.5g, magnesium sulfate heptahydrate 0.5g, ferrous sulfate heptahydrate 0.01g, agar 20g, distilled water 1000ml, natural pH.

[0014] Shake flask medium: glucose 100g, bean cake powder 30g, corn steep liquor 30g, distilled water 1000ml, natural pH.

[0015] Seed medium: 180g cornstarch, 30g bean cake powder, 30g corn syrup, 1000ml distilled water, natural pH.

[0016] Fermentation medium: 30% corn starch, 4% bean cake powder, 4% corn steep liquor, (NH 4 ) 2 SO 4 0.5%, natural pH.

[0017] First culture the strain on the slope of the slant medium at 33°C for 96h, then insert it into the shake flask containing the shake flask medium and cultivate it at 30°C for 48h, then insert it into the seed culture tank containing the seed medium at 32°C, 240rpm Cultivate for 48h. ...

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Abstract

The invention discloses a process for improving fermentation conversion rate of an enzyme preparation, which comprises steps of strain culture of enzyme-producing strains, aerobic fermentation culture and extraction and purification of the enzyme preparation, wherein oxygen-rich sterile air is introduced in a fermentation tank culture process of the aerobic fermentation culture step, or oxygen-rich sterile air is introduced in a seed tank culture process of the strain culture step and a fermentation tank culture process of the aerobic fermentation culture step. When the process meets the oxygen requirement of the strains, the process can improve the activity of the fermentation enzyme by over 10 percent, shorten the fermentation period by about 20 percent under the condition of smaller ventilation ratio, increase the charging coefficient of a reaction tank at the same time, improve the yield of the enzyme preparation by about 50 percent, and save the power consumption of ton product under the equal condition by about 40 percent compared with the power consumption without adopting the oxygen-rich sterile air so as to greatly improve the utilization rate of energy.

Description

technical field [0001] The invention belongs to the field of biochemistry, and in particular relates to a method for improving the fermentation conversion rate of enzyme preparations. Background technique [0002] The enzyme preparation industry is an important part of bioengineering. Its application fields cover light industry, food, chemical industry, medicine and health, agriculture, energy, and environmental protection. It plays an important role in the development of the national economy and has generated huge social and economic benefits. benefit. [0003] Due to the high oxygen demand during the aerobic fermentation of the strain, the large-scale production of enzyme preparations requires a large amount of ventilation to supply oxygen to the microorganisms, thereby increasing the dissolved oxygen level in the fermentation broth. In the prior art, the method of increasing the stirring speed or the ventilation ratio is usually used to increase the dissolved oxygen leve...

Claims

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Application Information

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IPC IPC(8): C12N9/50C12N9/26C12N9/34C12N9/42C12R1/125C12R1/69C12R1/685C12R1/885
Inventor 郭鸿飞周永治肖光焰黄荣强
Owner 江苏博立生物制品有限公司
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