Process for improving fermentation conversion rate of enzyme preparation

Abstract

The invention discloses a process for improving fermentation conversion rate of an enzyme preparation, which comprises steps of strain culture of enzyme-producing strains, aerobic fermentation culture and extraction and purification of the enzyme preparation, wherein oxygen-rich sterile air is introduced in a fermentation tank culture process of the aerobic fermentation culture step, or oxygen-rich sterile air is introduced in a seed tank culture process of the strain culture step and a fermentation tank culture process of the aerobic fermentation culture step. When the process meets the oxygen requirement of the strains, the process can improve the activity of the fermentation enzyme by over 10 percent, shorten the fermentation period by about 20 percent under the condition of smaller ventilation ratio, increase the charging coefficient of a reaction tank at the same time, improve the yield of the enzyme preparation by about 50 percent, and save the power consumption of ton product under the equal condition by about 40 percent compared with the power consumption without adopting the oxygen-rich sterile air so as to greatly improve the utilization rate of energy.

Description

technical field [0001] The invention belongs to the field of biochemistry, and in particular relates to a method for improving the fermentation conversion rate of enzyme preparations. Background technique [0002] The enzyme preparation industry is an important part of bioengineering. Its application fields cover light industry, food, chemical industry, medicine and health, agriculture, energy, and environmental protection. It plays an important role in the development of the national economy and has generated huge social and economic benefits. benefit. [0003] Due to the high oxygen demand during the aerobic fermentation of the strain, the large-scale production of enzyme preparations requires a large amount of ventilation to supply oxygen to the microorganisms, thereby increasing the dissolved oxygen level in the fermentation broth. In the prior art, the method of increasing the stirring speed or the ventilation ratio is usually used to increase the dissolved oxygen leve...

AI Technical Summary

Problems solved by technology

However, the above methods still cannot fully meet the large-scale production needs of enzyme preparations.

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