Method for improving fermentation conversion rate of enzyme preparation by utilizing oxygen partial pressure

Abstract

The invention discloses a method for improving the fermentation conversion rate of an enzyme preparation by utilizing oxygen partial pressure. The method comprises the steps of strain culture of an enzyme producing bacterial strain, aerobic fermentation culture, and extraction and purification of the enzyme preparation; oxygen-enriched sterile air is introduced in the fermentation tank culture process in the aerobic fermentation culture step or oxygen-enriched sterile air is introduced in the seed tank culture process in the strain seed culture step and the fermentation tank culture process inthe aerobic fermentation culture step. The method disclosed by the invention has the benefits that while the oxygen demand of a bacterial strain is ensured, the fermentation enzyme activity can alsobe improved by above 10 percent, and the fermentation period can also be shortened by about 20 percent under the condition of smaller ventilation ratio; moreover, the loading coefficient of the reaction tank is increased, the yield of the enzyme preparation is improved by about 50 percent, and under the same conditions, the power consumption of tons of products can be saved by about 40 percent than that when the oxygen-enriched sterile air is not adopted, so that the energy utilization rate is greatly improved.

Description

technical field [0001] The invention belongs to the field of biochemistry, and in particular relates to a method for improving the fermentation conversion rate of an enzyme preparation. Background technique [0002] The enzyme preparation industry is an important part of bioengineering. Its application fields cover light industry, food, chemical industry, medicine and health, agriculture, energy, and environmental protection. It plays an important role in the development of the national economy and has generated huge social and economic benefits. benefit. [0003] Due to the high oxygen demand during the aerobic fermentation of the strain, large-scale production of enzyme preparations requires a large amount of ventilation to supply oxygen to the microorganisms, thereby increasing the dissolved oxygen level in the fermentation broth. In the prior art, the method of increasing the stirring speed or the ventilation ratio is usually used to increase the dissolved oxygen level,...

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Problems solved by technology

However, the above methods still cannot fully meet the large-scale production needs of enzyme preparations.

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