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Tissue culture method of floral leaf pampasgrass

A technology of tissue culture and pampas grass, which is applied in the field of tissue culture of pampas grass, which can solve the problems of limited supply of seedlings, small number of introduced pampas grass, etc., to improve stability, increase speed of reproduction and neatness of seedlings degree of effect

Inactive Publication Date: 2010-10-27
SHANGHAI SHANGFANG LANDSCAPE PLANT INST +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as a new variety introduced from abroad, the number of introduced species of Pampas syringae is relatively small, and the supply of seedlings is limited to a certain extent.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Obtaining sterile materials

[0029] Take the young buds that have germinated in spring, remove the wrapped leaves, wash them with tap water for 1 hour, put them on the ultra-clean workbench, soak them in ethanol with a mass concentration of 70% for 10 seconds, and then soak them in mercury with a volume concentration of 0.5‰ for 10 minutes. Rinse 4 times with sterile water again, use sterile filter paper to blot the moisture on the surface, then cut into 0.5cm-long segments with axillary buds, and inoculate the segments with MS+6-BA1.0mg / L+NAA0. On the axillary bud induction medium of 1mg / L;

[0030] (2) Differentiation and proliferation of buds

[0031] After the segments were inoculated on the axillary bud induction medium for 1 week, the axillary buds began to expand and green protrusions appeared. After 3 weeks, bud meristems could be seen. After culturing for 1 month, the small adventitious buds could grow to 3 cm. Cut the adventitious buds Under transfer in...

Embodiment 2

[0040] (1) Obtaining sterile materials

[0041] Take the young buds that have germinated in spring, remove the wrapped leaves, wash them with tap water for 2 hours, put them on an ultra-clean workbench, and soak them in ethanol with a mass concentration of 75% for 30 seconds, and mercury with a volume concentration of 1‰ for 15 minutes. Rinse 5 times with sterile water, use sterile filter paper to blot the water on the surface, then cut into 1cm-long segments with axillary buds, and inoculate the segments with MS+6-BA2.0mg / L+NAA0.2mg / L on the axillary bud induction medium;

[0042] (2) Differentiation and proliferation of buds

[0043] After the segments were inoculated on the axillary bud induction medium for 2 weeks, the axillary buds began to expand and green protrusions appeared. After 4 weeks, bud meristems could be seen. After culturing for 1 month, the small adventitious buds could grow to 4cm. Cut the adventitious buds Under transfer into the adventitious bud prolif...

Embodiment 3

[0052] (1) Obtaining sterile materials

[0053] Take the young buds that have germinated in spring, remove the leaves wrapped outside, rinse them with tap water for 3 hours, put them on the ultra-clean workbench, and soak them in ethanol with a mass concentration of 75% for 50 seconds, and mercury with a volume concentration of 2‰ for 30 minutes. Rinse 6 times with sterile water, use sterile filter paper to blot the water on the surface, then cut into 2cm-long segments with axillary buds, and inoculate the segments with MS+6-BA5.0mg / L+NAA0.5mg / L on the axillary bud induction medium;

[0054] (2) Differentiation and proliferation of buds

[0055] After the segments were inoculated on the axillary bud induction medium for 3 weeks, the axillary buds began to expand and green protrusions appeared. After 5 weeks, bud meristems could be seen. After 2 months of culture, the small adventitious buds could grow to 4cm. Cut the adventitious buds Under transfer into the adventitious bud ...

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Abstract

The invention relates to a tissue culture method of floral leaf pampasgrass, comprising the steps of obtaining sterile materials, splitting and breeding germ, cultivating adventitious bud strong seedling, cultivating root, hardening seedling, replanting, and the like. Compared with the prior art, the invention greatly improves the breeding speed of floral leaf pampasgrass and the uniformity of the seedling, improves the stability of the character and can realize factory and batch production of grow seedlings.

Description

technical field [0001] The invention relates to a plant tissue culture method, in particular to a tissue culture method of pampas grass. Background technique [0002] Pampagne mosaic is a plant of the genus Pampas grass, widely distributed, and is an evergreen perennial herb with a plant height of 80-100cm, leaves with white stripes, inflorescences conical and feathery, silvery white, and is a famous ornamental grass abroad. It is mostly used for landscaping or shore. The leaves and flower spikes of Pampas reed are beautiful, and the garden cultivation is spectacular and elegant. When planted on the bank, you can enjoy the panicle of silvery white pinnate spikes in autumn. It can also be used as dried flowers or flower border ornamental grasses in the special garden It has excellent ecological adaptability and ornamental value. However, as a new variety introduced from abroad, the number of introduced species of Pampas reed is relatively small, and the supply of seedlings ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 陈建华黄建荣沈勤
Owner SHANGHAI SHANGFANG LANDSCAPE PLANT INST
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