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Tissue culture method of African agapanthus

A technology of tissue culture and Agapanthus, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of small number of introduced Agapanthus species, slow branching of seed bulbs, limited supply of seedlings, etc., and achieve improved reproduction Speed, the effect of improving the uniformity of seedlings

Inactive Publication Date: 2010-10-27
SHANGHAI SHANGFANG LANDSCAPE PLANT INST +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a new variety introduced from abroad, the number of introduced species of Agapanthus is small, the bulbs are slow to ramify, and the supply of seedlings is limited

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Obtaining sterile materials

[0029] When the Agapanthus sibiricus blooms, pick the unflowered buds, rinse them with tap water for 1 hour, and then soak them in ethanol with a mass concentration of 60% for 10 seconds and mercury with a volume concentration of 0.5‰ for 10 minutes. Rinse with bacterial water 4 times, absorb the surface water with sterile filter paper, inoculate the buds on the axillary bud induction medium including MS+6-BA1.0mg / L+NAA0.1mg / L;

[0030] (2) Differentiation and proliferation of bulbs

[0031] The flower buds were inoculated on the flower bud induction medium. After 1 week, the flower buds began to expand and appeared white protrusions. After 3 weeks, the bud meristems were visible. After another month of cultivation, small bulbs were formed. Cut the small bulbs and move them into MS+6. -BA0.5mg / L+NAA0.1mg / L corm growth medium for proliferation culture, there are more callus at the base of the bulb, but the bulb grows well, and there is no adve...

Embodiment 2

[0040] (1) Obtaining sterile materials

[0041] When the Agapanthus sibiricus blooms, pick the unflowered flower buds, rinse them with tap water for 1 hour, and then soak them in ethanol with a mass concentration of 75% for 30 seconds and mercury with a volume concentration of 1‰ for 15 minutes on an ultra-clean workbench. Rinse with bacteria water 5 times, absorb the surface water with sterile filter paper, inoculate the flower buds on axillary bud induction medium including MS+6-BA5.0mg / L+NAA0.5mg / L;

[0042] (2) Differentiation and proliferation of bulbs

[0043] The flower buds were inoculated on the flower bud induction medium. After 3 weeks, the flower buds began to expand and appeared white protrusions. After 4 weeks, the bud meristems were visible. After another one and a half months of cultivation, small bulbs were formed. Cut the small bulbs and move them into MS+6. -BA2.0mg / L+NAA0.2mg / L corm growth medium for proliferation culture, there are more callus at the base of the...

Embodiment 3

[0052] (1) Obtaining sterile materials

[0053] When the Agapanthus sibiricus blooms, pick the unflowered flower buds, rinse them with tap water for 3 hours, and then soak them in ethanol with a mass concentration of 75% for 50 seconds and mercury with a volume concentration of 2‰ for 30 minutes on the ultra-clean workbench. Rinse with bacteria water 6 times, absorb the surface water with sterile filter paper, inoculate the flower buds on the axillary bud induction medium including MS+6-BA5.0mg / L+NAA0.5mg / L;

[0054] (2) Differentiation and proliferation of bulbs

[0055] The flower buds were inoculated on the flower bud induction medium. After 3 weeks, the flower buds began to expand and appeared white protrusions. After 5 weeks, the bud meristems were visible. After another 2 months of cultivation, small bulbs were formed. Cut the small bulbs and move them into MS+6. -BA4.0mg / L+NAA0.3mg / L corm growth medium for proliferation and culture, there are more callus at the base of the bu...

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PUM

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Abstract

The invention relates to a tissue culture method of African agapanthus, comprising the following steps of: acquisition of a sterile material, differentiation and propagation of corms, strong seedling culture of corms, rooting culture, seedling hardening, transplanting, and the like. Compared with the prior art, the invention greatly improves the propagation speed of the African agapanthus and the neatness of seedlings and can realize industrial large-batch production of the seedlings.

Description

Technical field [0001] The present invention relates to a plant tissue culture method, in particular to a tissue culture method of Agapanthus. Background technique [0002] Agapanthus is a plant of the genus Agapanthus in the Amaryllidaceae family. It is native to Peru and Brazil and is now widely cultivated. Agapanthus is a perennial herbaceous plant with thick, nearly spherical bulbs, 5-7cm in diameter, and light green or yellowish-brown skin. The leaves are opposite on both sides, band-shaped, with a tapered apex, 6-8 leaves in total, and more leaves emerge after the flowers. There are 2-4 flowers on the top. The flowers are huge and purple in color. They are good flower mirrors and garden materials. As a new variety introduced from abroad, the number of introductions of Agapanthus agapanthus is small, the bulbs are divided slowly, and the supply of seedlings is limited. Contents of the invention [0003] The object of the present invention is to provide a tissue cul...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 陈建华黄建荣沈勤
Owner SHANGHAI SHANGFANG LANDSCAPE PLANT INST
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