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Porcine circovirus-II vaccine potency test method

A technology for porcine circovirus and an inspection method, which is applied in the field of porcine circovirus type 2 vaccine efficacy inspection, can solve the problems of expensive PCV2 challenge model establishment, time-consuming piglet challenge experiment method, large batch-to-batch variation of inspection results, and the like, Achieve significant differences in experimental results, short detection time, and improve efficiency.

Inactive Publication Date: 2010-10-27
PU LIKE BIO ENG
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Problems solved by technology

However, the PCV2 pollution of pigs in my country is serious, and it is difficult to screen out qualified experimental pigs for PCV2 vaccine development or vaccine efficacy testing, and the piglet challenge experiment method is time-consuming, laborious, expensive, and it is difficult to establish a PCV2 challenge model. Large batch-to-batch differences in results

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Detection of Porcine Circovirus Type 2 Inactivated Vaccine Efficacy

[0031] 1. Source of inactivated vaccine

[0032] The virus strain of PCV2 inactivated vaccine is PCV2-SH strain (preservation number CGMCCNo.2389). The PCV2-SH strain was inoculated into PK15 cells, and the virus antigen solution was harvested 5 days later as an inactivated vaccine.

[0033] The prepared inactivated vaccines were divided into three groups Ⅰ, Ⅱ, and Ⅲ, and their antigen contents were determined to be 10 5.0 TCID 50 / ml, 10 5.5 TCID 50 / ml, 10 6.0 TCID 50 / ml. Next, three groups of inactivated vaccines were used as samples to immunize mice to determine their serum antibody levels.

[0034] 2. Antibody detection in immunized mice

[0035] (1) Immunization of BALB / c mice

[0036] The healthy BALB / c mice aged 5-6 weeks were randomly divided into 4 groups, 5 mice in each group, and the antigen content of 10 5.0 TCID 50 / ml porcine circovirus type 2 inactivated vaccine immunizati...

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Abstract

The invention belongs to the technical field of veterinary medicaments and new biological medicaments and relates to a porcine circovirus-II vaccine potency test method. The method comprises: immunizing a white mouse with a porcine circovirus-II inactivated vaccine; and determining the PCV2 specific antibody content of the serum of the white mouse to evaluate the potency of the vaccine. When the vaccine potency test method is used, and the problems of time-consuming and labor-consuming test pig screening, large inter-batch test result difference and the like of the conventional porcine circovirus-II inactivated vaccine potency test are solved. The test method of the invention has the advantages of short test period, simple operation, low cost, accurate result and high repeatability. The method has a high application value and a promising market prospect.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for testing the efficacy of porcine circovirus type 2 vaccine. Background technique [0002] Porcine cirovirus type 2 (PCV2) is the main pathogen that causes post-weaning multisystemic wasting syndrome (PMWS) in piglets. Since its outbreak in Canadian swine herds in 1991, PMWS has spread globally, causing huge economic losses to the swine industry worldwide. PCV2 has also been associated with hyperplastic necrotizing pneumonia, reproductive failure in sows, porcine dermatitis and nephrotic syndrome, and porcine respiratory disease syndrome. Porcine circovirus-associated disease (PCV2 Associated Disease, PCVD), mainly PMWS, caused a loss of up to 600 million euros in Europe in 2005. PCV2 infectious disease causes increased mortality, lower feed remuneration, and the level of feeding and management of large-scale pig farms in my country is relatively backward, so PCVD has al...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569
CPCG01N33/56983G01N2333/01
Inventor 张许科孙进忠乔荣岑
Owner PU LIKE BIO ENG
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