Technology for producing calf chymosin substitute of cheese
A rennet and substitute technology, which is applied in the technical field of production and preparation of cheese calf rennet substitute, can solve the problems of strong degradability of casein protein, etc., and achieves the promotion of rennet production, high fermentation activity, guaranteeing yield effect
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Embodiment 1
[0014] Embodiment 1: (a kind of production preparation technology of cheese calf rennet substitute)
[0015] 1) Prepare medium: 25g of glutinous rice, 10g of bran, add animal hydrolyzed protein, skimmed milk powder, soybean protein, etc., the addition amount is 2g, 0.25g, 0.3g respectively. After mixing the raw materials evenly, dilute to 100g with water, pH6.8±0.2, steam sterilization at 121°C for 15min, cool down after sterilization and inoculate. The fermentation time is 72h.
[0016] 2), strain activation: the strains preserved through freeze-drying are not vigorous,
[0017] In a dormant state that sustains life, especially freeze-dried bacteria. When the bacteria are subjected to severe physical stimulation during freeze-drying, most of them die, and only a few percent survive. Therefore, they need to be activated before use to restore their vitality to normal. Take it directly with a sterilized coiled platinum earring, and transfer it into a fresh medium test tube. ...
Embodiment 2
[0019] Embodiment 2: (a kind of production preparation technology of cheese calf rennet substitute)
[0020] 1) Prepare medium: 400g glutinous rice, 100g bran, add animal hydrolyzed protein, skimmed milk powder, soybean protein, etc., and add 20g, 1.5g, 1.5g respectively. Ammonium citrate and ammonium sulfate, the added amount is 0.5g and 1g. After mixing the raw materials evenly, pH6.8±0.2, steam sterilization at 121°C for 15 minutes, after sterilization, cool down and inoculate. The fermentation time is 72h.
[0021] 2) Activation of strains: Freeze-dried strains, preserved strains are not vigorous, and are in a dormant state to maintain life, especially freeze-dried strains. The strains were subjected to intense physical stimulation during freeze-drying, most of them died, and only a few percent survived. Therefore, they need to be activated before use to restore their vitality to normal. Take it directly with a sterilized coiled platinum earring, and transfer it into a ...
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