Method for preparing hydrogen-producing and ethanol-producing microbial aggregate

Abstract

The invention relates to a method for preparing a hydrogen-producing and ethanol-producing microbial aggregate, which comprises the following steps of: preprocessing inoculated anaerobic sludge, and introducing synthetic organic waste water; and when the degradation rate of glucose in outflow water of a UASB reactor reaches over 95 percent, gradually increasing the concentration of an organic matter in inflow water and keeping the pH value of the outflow water to be between 4 and 4.5. In the invention, starting with the realization of a synchronous hydrogen-producing and ethanol-producing technique of the organic waste water, the diversity of microbes in the mature hydrogen-producing and ethanol-producing microbial aggregate is analyzed; a clone technique and a sequencing technique are combined for studying the functional group of the microbe and analyzing to obtain a population structure of the optimal hydrogen-producing and ethanol-producing microbes, so as to provide theory and technique guide for artificially constructing an anaerobic, high-efficiency biological hydrogen production system along with stable operation and accelerate the industrialization of organic waste water resource utilization.

Description

technical field [0001] The invention relates to a preparation method of microbial aggregates, in particular to a preparation method of hydrogen-producing ethanol-producing microbial aggregates. Background technique [0002] At present, edible substances such as sugar cane and corn sugar and starch are widely used as raw materials for the production of bioethanol. The limited supply and high cost limit its application. The production of hydrogen is mainly through chemical methods, which consumes a lot of energy and may cause environmental pollution. It is also facing the problem of how to effectively reduce costs and reduce environmental pollution. Biological treatment is mostly used for organic wastewater treatment. Traditional organic wastewater biological treatment technologies (anaerobic technology, aerobic technology) focus on decomposing organic matter into carbon dioxide and water, but fail to realize its resource utilization. [0003] Many organic wastewaters, such a...

AI Technical Summary

Problems solved by technology

In 2005, the "Organic Wastewater Fermentation Biological Hydrogen Production Technology Demonstration Project" was launched in Harbin International Science and Technology City, with a daily hydrogen production of 1200m 3 , but the cost of fermentation biohydrogen production is still high (Das and Veziroglu, 2009)

Similarly, to realize the continuous and stable operation of the UASB reactor for synchronous hydrogen production and ethanol production from organic wastewater, the best way is to quickly obtain this type of fermentation type of hydrogen-producing microbial aggregates, but there are few reports on this aspect of research

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