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Shearing force-electricity combined stimulation cell culture device

A technology of cell culture and shear force, which is applied in the field of cell engineering and tissue engineering, can solve undiscovered problems, achieve simple operation, improve cell growth conditions, and have good biocompatibility

Inactive Publication Date: 2010-11-24
BEIHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no culture device that can simultaneously apply force and electrical stimulation to cells in China.

Method used

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  • Shearing force-electricity combined stimulation cell culture device
  • Shearing force-electricity combined stimulation cell culture device
  • Shearing force-electricity combined stimulation cell culture device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Culture of Cardiomyocytes Combined with Shear Force-Electric Stimulation

[0039] Under sterile conditions, the cardiomyocytes of 1-3 day old SD rat suckling mice were divided into 1×10 5 / cm 2 The density is seeded on the plate electrode 603, and the plate electrode is made of tin dioxide conductive glass doped with fluorine. After 3 days of pre-cultivation, the plate electrode 603 inoculated with cardiomyocytes is placed on the plexiglass base 604, and then the silica gel gasket 602 is placed on the On the plate electrode 603, then put the plexiglass cover 601 on the top, and finally fix the plexiglass cover 601 and the plexiglass base 604 with screws.

[0040] Pass the downstream liquid storage bottle 3 and the upstream liquid storage bottle 4 after autoclaving through three silicone tubes such as figure 1 connected as shown. Then the upstream liquid storage bottle 4 is connected to the fluid inlet 606 through a silicone tube, and the downstream liquid...

Embodiment 2

[0043] Example 2 Culture of endothelial cells stimulated by combined shear force and electricity

[0044] Under sterile conditions, the human umbilical vein endothelial cells were divided into 1 × 10 5 / cm 2 The density is seeded on the plate electrode 603, such as indium tin oxide conductive glass. The next day after the cells adhere to the wall, the plate electrode 603 inoculated with endothelial cells is placed on the plexiglass base 604, and then the silicone gasket 602 is placed on the plate electrode 603. Then the plexiglass cover 601 is placed on the top, and finally the plexiglass cover 601 and the plexiglass base 604 are fixed with screws.

[0045] Pass the downstream liquid storage bottle 3 and the upstream liquid storage bottle 4 after autoclaving through three silicone tubes such as figure 1 connected as shown. Then the upstream liquid storage bottle 4 is connected to the fluid inlet 606 through a silicone tube, and the downstream liquid storage bottle 3 is co...

Embodiment 3

[0048] Example 3 Culture of Osteoblasts Stimulated by Combined Shear Force and Electricity

[0049] Under sterile conditions, the osteoblast MC3T3 cells were divided into 1 × 10 5 / cm 2 Density seeding on the plate electrode 603, such as conductive glass deposited with conductive polypyrrole, the next day after the cells adhere to the wall, place the plate electrode 603 seeded with osteoblasts on the plexiglass base 604, and then place the silicone gasket 602 on the plate On the electrode 603, then the plexiglass cover 601 is placed on the top, and finally the plexiglass cover 601 and the plexiglass base 604 are fixed with screws.

[0050] Pass the downstream liquid storage bottle 3 and the upstream liquid storage bottle 4 after autoclaving through three silicone tubes such as figure 1 connected as shown. Then the upstream liquid storage bottle 4 is connected to the fluid inlet 606 through a silicone tube, and the downstream liquid storage bottle 3 is connected to the flu...

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Abstract

The invention provides a shearing force-electricity combined stimulation cell culture device, which mainly comprises an incubator, a peristaltic pump, a downstream liquid storage bottle, an upstream liquid storage bottle, a signal generator and a flat flowing cavity. The peristaltic pump and the downstream liquid storage bottle are connected with components such as upstream liquid storage bottle and the like through silicone tubes to form a culture solution perfusion system for providing nutrition required for cell growth and loading shearing force stimulation to cells; the signal generator is used for providing electrical signals for loading electricity stimulation to the cells; and the flat flowing cavity is used for providing a substrate required for the cell growth and connected with the signal generator and the culture solution perfusion system to provide a shearing force-electricity combined acting environment for the cells on a flat electrode. The device establishes a cell culture environment of combining force stimulation and electricity stimulation, overcomes the defect that the prior art cannot simulate in vivo cells in the force-electricity combined acting environment, and lays a foundation for promoting functionalized culture of the cells and tissues.

Description

technical field [0001] The invention relates to the fields of cell engineering and tissue engineering, in particular to a cell culture device that combines a mechanical environment and an electrical environment. Background technique [0002] In tissue engineering, the in vitro functional culture of cells and tissues has become the core of tissue engineering and the necessary technical basis for the formation of tissue engineering industry. It is very important for the three-dimensional culture and functionalization of cells and tissues to provide an in vitro growth environment similar to the living conditions of cells in vivo. Among them, physical influence factors such as force field and electric field play a role that cannot be ignored. [0003] The mechanical environment has an important impact on life activities at all levels of organs, tissues, cells and even sub-cells. For example, the shear force generated by the blood flow directly acts on the endothelial cells and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/42
CPCC12M35/02C12M29/10
Inventor 樊瑜波李萍牛旭锋冯利敏李欣蔚贾璐
Owner BEIHANG UNIV
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