Pichiacaribbica, application thereof in fruit storage and refreshment and use method thereof

A technology for storage and preservation of yeast, which is applied in the field of Pichia calibicus, can solve problems affecting the use of antagonistic bacteria, and achieve the effects of reducing losses, inhibiting pathogenic bacteria, and preventing rot

Active Publication Date: 2010-12-01
无棣鑫岳化工集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These factors have affected the use of antagonistic bacteri

Method used

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  • Pichiacaribbica, application thereof in fruit storage and refreshment and use method thereof
  • Pichiacaribbica, application thereof in fruit storage and refreshment and use method thereof
  • Pichiacaribbica, application thereof in fruit storage and refreshment and use method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1: Utilize Pichia kalibicus to carry out fruit (pome fruit) post-harvest disease prevention and storage method

[0026] The activated yeast was inoculated in a conical flask filled with 50ml of NYDB (5g of yeast extract, 8g of beef extract, 10g of glucose, 1000ml of purified water, pH7.0-9.0), and cultured on a shaking table at 28°C and 180r / min for 48h. Take the bacterial solution and centrifuge it at 4000r / min for 10min, discard the supernatant, wash it repeatedly with sterile water for 3 times, and count it on a hemocytometer to obtain a concentration of 1×10 8 cells / ml of bacterial suspension. Punch 3 holes with a diameter of 3m (width) × 3mm (depth) at the waist of the fruit, and inject 30 μl of antagonistic bacteria at a concentration of 1 × 10 in each hole.8 cells / ml cell suspension, the control fruit was injected with 30 μl sterile water. After 2 hours, 30 μl of spore suspensions of Botrytis cinerea and Penicillium expansum were inoculated respective...

Embodiment 2

[0031] Embodiment 2: Utilize Pichia calibicus to carry out post-harvest disease prevention and storage and fresh-keeping methods of fruit (strawberry)

[0032] The activated yeast was inoculated in a conical flask filled with 50ml of NYDB (5g of yeast extract, 8g of beef extract, 10g of glucose, 1000ml of purified water, pH7.0-9.0), and cultured on a shaking table at 28°C and 180r / min for 48h. Take the bacterial solution and centrifuge it at 4000r / min for 10min, discard the supernatant, wash it repeatedly with sterile water for 3 times, and count it on a hemocytometer to obtain a concentration of 1×10 7 pcs / ml, 1×10 8 pcs / ml, 1×10 9 cells / ml of bacterial suspension, and the control group was sterile water. Punch 2 holes with a diameter of 3m (width) × 3mm (depth) at the waist of the fruit, and then inject 30 μl of the above-concentrated antagonistic antibiotic suspension into each hole, and inject 30 μl of sterile water into each hole. After 2 hours, 30 μl of the spore susp...

Embodiment 3

[0037] Embodiment 3: Utilize Pichia calibicus to carry out post-harvest disease prevention and storage and fresh-keeping methods of fruit (strawberry)

[0038] 1. Test plan

[0039] The activated yeast was inoculated in a conical flask filled with 50ml of NYDB (5g of yeast extract, 8g of beef extract, 10g of glucose, 1000ml of purified water, pH7.0-9.0), and cultured on a shaking table at 28°C and 180r / min for 48h. Take the bacterial solution and centrifuge it at 4000r / min for 10min, discard the supernatant, wash it repeatedly with sterile water for 3 times, and count it on a hemocytometer to obtain a concentration of 1×10 7 cells / ml of bacterial suspension. Soak strawberries to 1 x 10 7 Individual / ml bacterial suspension, contrast strawberry is soaked in sterile water, and the time is 30 seconds-2 minutes. After drying, put the fruit into a plastic basket, seal it with plastic wrap to maintain a humidity of 95%, and store it at room temperature. After 5 days, the incidenc...

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Abstract

The invention discloses a Pichiacaribbica which can prevent diseases of picked fruit, an application thereof in fruit storage and refreshment and a use method thereof. The Pichiacaribbica is CGMCC No.3616. A thallus can be obtained by activating Pichiacaribbica, fermenting and centrifuging. The thallus is diluted with sterile water so as to prepare the thallus suspension with a concentration of between 1.0*107 pieces/ml and 1.0*109 pieces/ml. After the fruit is put into the thallus suspension and soak for 30 seconds to 2 minutes, the fruit can be taken out immediately, dried, and then put into a plastic crate and sealed with a plastic wrap. The invention uses the Pichiacaribbica to prevent the diseases of picked fruit, the use is simple, the operation is convenient, the effect is excellent and the cost is low. Besides, the invention can prevent the harm to human by using chemical fungicide and has obvious economic benefits and social benefits.

Description

technical field [0001] The invention relates to Pichiacaribbica with control effect on post-harvest fruit diseases, application of the bacterial strain in fruit storage and fresh-keeping and its application method. Background technique [0002] The loss caused by rot in the process of fruit picking, transportation and storage is huge, and it is also a problem of global concern. According to reports, in developed countries, 10%-30% of fresh products are rotted due to post-harvest diseases, while in developing countries that lack storage and transportation refrigeration equipment, the rate of post-harvest fruit rot is as high as 40%-50%. The storage and preservation of foreign fruits accounts for more than 60% of the total fruit output. At present, the storage capacity of fruit in my country is more than 8 million tons, which is less than 15% of the total. Among them, the storage capacity of cold storage and air-conditioning storage is 700,000 tons, accounting for only 10% of ...

Claims

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Application Information

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IPC IPC(8): C12N1/16A23B7/155C12R1/84
Inventor 张红印庞水秀张晓云任晓锋
Owner 无棣鑫岳化工集团有限公司
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