Method for reducing ammonium nitrogen content in L-isoleucine fermentation waste liquor

A technology for fermenting waste liquid and isoleucine, which is applied in microorganism-based methods, fermentation, and biochemical equipment and methods, etc., can solve the problems of treating the symptoms but not the root cause, and it is difficult to offset the investment and operation costs of engineering construction funds. The effect of reducing nitrogen content and ammonia consumption

Active Publication Date: 2011-07-20
WUXI JINGHAI AMINO ACID
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Problems solved by technology

This treatment method is a negative pollution control method that treats the symptoms but not the root cause, especially for a large amount of industrial high-concentration wastewater, it is difficult to cure; from a technical point of view, the development of terminal treatment technology is no less difficult than the development of production process technology , and the bigger problem is the economic problem. The small amount of output of the end treatment technology is difficult to offset the capital investment of the project construction and the daily expensive operation costs. The larger the scale of production, the more unbearable it is

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  • Method for reducing ammonium nitrogen content in L-isoleucine fermentation waste liquor

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Effect test

Embodiment 1

[0014] Put 3.0 liters of fermentation liquid in a 5-liter fermenter, inoculate with 5% seed liquid, add ammonia water with a mass concentration of 50% after 8 hours of fermentation to control the pH to 7.2-7.5, stop feeding ammonia water after 40 hours of fermentation, and change to continuous feeding 0.1mol / L sodium hydroxide solution controls the pH to 7.2-7.5, and when the fermentation ends, the output of L-isoleucine is 28.4g / L. Compared with the existing technology, the amount of ammonia water is reduced by about 50%, and the content of ammonium nitrogen in the fermentation broth is reduced from about 4000mg / L to about 600mg / L.

Embodiment 2

[0016] Put 350 liters of fermentation liquid in a 500-liter fermenter, inoculate with 5% seed liquid, add ammonia water with a mass concentration of 70% after 10 hours of fermentation to control the pH to 7.2-7.5, stop feeding ammonia water after 42 hours of fermentation, and change to feeding 0.5mol / L sodium hydroxide solution controls the pH to 7.2-7.5, and when the fermentation ends, the output of L-isoleucine is 28.7g / L. Compared with the existing technology, the amount of ammonia water is reduced by about 50%, and the content of ammonium nitrogen in the fermentation broth is reduced from 4000mg / L to about 500mg / L.

Embodiment 3

[0018] Put 3500 liters of fermentation liquid in a 5000-liter fermenter, inoculate with 5% seed liquid, add ammonia water with a mass concentration of 80% after 10 hours of fermentation to control the pH to 7.2-7.5, stop feeding ammonia water after 39 hours of fermentation, and change to feeding 2mol / L sodium hydroxide solution controls the pH to 7.2-7.5, and when the fermentation ends, the output of L-isoleucine is 29.1g / L. Compared with the existing technology, the amount of ammonia water is reduced by about 50%, and the content of ammonium nitrogen in the fermentation broth is reduced from 4000mg / L to about 600mg / L.

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Abstract

The invention relates to a method for reducing the ammonium nitrogen content in L-isoleucine fermentation waste liquor, in particular to a clean production method of L-isoleucine and belongs to the technical field of biochemical engineering. The method is mainly to use sodium hydroxide solution to partially replace the fed ammonia water in the fermentation process of L-isoleucine so as to supplement nitrogen source and control the pH value, and reduce the dosage of ammonia water and the content of ammonium nitrogen while not reducing the yield of L-isoleucine. Compared with the prior art, thebest advantage of the invention is to greatly reduce the ammonium nitrogen content in the fermentation waste liquor, thus the ammonium nitrogen content in L-isoleucine fermentation waste liquor can be reduced by above 80% and the dosage of ammonia water can be reduced by above 50%.

Description

technical field [0001] The invention discloses a method for reducing the content of ammonium nitrogen in L-isoleucine fermentation waste liquid, specifically relates to a clean production method of L-isoleucine, and belongs to the technical field of biochemical industry. Background technique [0002] L-isoleucine is one of the eight essential amino acids for the human body and one of the three branched-chain amino acids. It has wide application and commercial value in the pharmaceutical and food industries. At present, L-isoleucine is mainly produced by fermentation. Production. During the fermentation process of L-isoleucine, intermittent flow of ammonia water is required to adjust pH and supplement nitrogen source. The L-isoleucine is produced by adopting the existing technology, and the content of ammonium nitrogen in the fermentation waste liquid is relatively high. At present, the treatment of ammonium-nitrogen-containing fermentation waste liquid adopts "terminal tre...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P13/06C12R1/13
Inventor 宁健飞蔡立明
Owner WUXI JINGHAI AMINO ACID
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