Method for ultrasonically extracting general flavone from chloranthus japonicus

A technology of Silverwort and total flavonoids, which can be used in pharmaceutical formulas, medical preparations containing active ingredients, anti-toxins, etc., and can solve the problems that silverwort has no relevant reports.

Inactive Publication Date: 2010-12-15
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no relevant report on the research on the extraction of effective components of Silverwort

Method used

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  • Method for ultrasonically extracting general flavone from chloranthus japonicus
  • Method for ultrasonically extracting general flavone from chloranthus japonicus
  • Method for ultrasonically extracting general flavone from chloranthus japonicus

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Embodiment Construction

[0016] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0017] 1 Materials and Instruments

[0018] (1) Experimental materials

[0019] Silverwort was collected from Taibai Mountain in Shaanxi in July 2007. It was identified as Chloranthus japonicus Sieb. by Professor Tian Xianhua, College of Life Sciences, Shaanxi Normal University. Sample specimens are preserved in the College of Life Sciences, Shaanxi Normal University.

[0020] (2) Instruments and reagents

[0021] KQ-600DB CNC ultrasonic cleaner (600w, 40kHz, Kunshan Ultrasonic Instrument Co., Ltd.), electronic balance, UV-2450 ultraviolet spectrophotometer (Shimadzu, Japan), FZ102 miniature plant pulverizer (Huanghua Zhongxing Co., Ltd.) ; Rutin was purchased from the China Institute for the Control of Pharmaceutical and Biological Products, methanol, petroleum ether, sodium nitrate and other reagents used were of analytical grade.

[00...

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Abstract

The invention discloses a method for ultrasonically extracting general flavone from chloranthus japonicas. The method is characterized by comprising the following steps of: smashing the chloranthus japonicas, sieving the smashed chloranthus japonicas through a 40-mesh screen, adding petroleum ether into the sieved chloranthus japonicas for uniform mixing, fully decolorizing the mixture, filtering the decolorized mixture with two layers of filter paper and vaporizing filter residues to dryness for later use; and correctly weighing a pre-treated material, adding 50 to 60 percent solution of methanol into the pre-treated material in a material-to-liquid ratio (g / mL) of 1:15-20, fully shaking the mixture, dipping for 1 hour and ultrasonically extracting at the temperature of between 55 and 65 DEG C and at the frequency of 40 KHz for 5 to 10 minutes.

Description

technical field [0001] The invention relates to a method for extracting plant effective components, in particular to a method for ultrasonically extracting total flavonoids of silvertracea. Background technique [0002] Response surface methodology (RSM) is an experimental optimization method that combines mathematical methods and statistical methods. It expresses implicit functional functions by approximately constructing a polynomial with a clear expression form, and is affected by multiple independent variables. Modeling and analyzing the response of the model, the ultimate goal is to optimize the response value. Due to the adoption of a more reasonable experimental design, the optimal conditions of the multi-factor system can be effectively and quickly determined in the most economical way. To put it simply, the response surface method is a test method that arranges all test points on a spherical surface. Under a limited number of experiments, a continuous variable surf...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/898A61P9/00A61P39/06
Inventor 康杰芳王喆之王慧李娜
Owner SHAANXI NORMAL UNIV
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