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Cynoglossus semilaevis gunther sex-linked microsatellite marker and genetics sex testing method

A technology of microsatellite marking and semi-smooth tongue sole, applied in the fields of microsatellite marking and genetic sex identification

Inactive Publication Date: 2010-12-15
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Abstract
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Problems solved by technology

However, there is no literature or patent report on sex-linkage or specific microsatellite marker screening of tongue sole in half-smooth tongue sole

Method used

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  • Cynoglossus semilaevis gunther sex-linked microsatellite marker and genetics sex testing method
  • Cynoglossus semilaevis gunther sex-linked microsatellite marker and genetics sex testing method
  • Cynoglossus semilaevis gunther sex-linked microsatellite marker and genetics sex testing method

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Embodiment Construction

[0029] Below by embodiment and in conjunction with accompanying drawing, technical content of the present invention is described in detail:

[0030] The present invention includes three aspects: 1. Screening of sex-linked microsatellite markers in tongue sole and its primer sequence; 2. Female-specific microsatellite allele sequence and primer sequence in tongue sole; 3. Genetic sex identification of tongue sole based on microsatellite markers application method.

[0031] 1. Sex-linked microsatellite markers and their primer sequences in half-smooth tongue sole: including: 1. Screening and cloning of microsatellite sequences; 2. Design of microsatellite primers; 3. Verification of sex-linked microsatellite markers.

[0032] 1. Screening and cloning of microsatellite sequences:

[0033] The fin of 1 half smooth tongue sole is about 5mm 2 Put it into a 1.5mL centrifuge tube, and cut it into pieces with clean surgical scissors after the ethanol evaporates. Add 700 μL extractio...

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Abstract

The invention relates to a cynoglossus semilaevis gunther sex-linked microsatellite marker and genetics sex testing method comprising a PCR method and a PCR testing method, wherein the PCR method is used for screening and testing of a cynoglossus semilaevis gunther sex-linked microsatellite marker, clone of female peculiar allelic gene, sequence analysis, peculiar primer design and cynoglossus semilaevis gunther genetics sex; and the PCR testing method is used for creating the cynoglossus semilaevis gunther genetics sex according to the obtained cynoglossus semilaevis gunther sex-linked microsatellite marker primer and female peculiar allelic gene. The invention ensures that the cynoglossus semilaevis gunther sex-linked microsatellite marker and the female peculiar allelic gene are screened for the first time, establishes the PCR technique tested by the cynoglossus semilaevis gunther genetics sex, has the characteristics of high efficiency, accuracy and stability, has important application value in the cynoglossus semilaevis gunther sex control and unisexuality seed production, and also has important application prospect in the sex determination gene research by cloning the cynoglossus semilaevis gunther.

Description

Technical field: [0001] The invention belongs to the genetic sex molecular marker identification technology of seawater fish in the technical field of aquatic organisms, and relates to a microsatellite marker linked with the genetic sex of half smooth tongue sole and a genetic sex identification method. Background technique [0002] Cynoglossus semilaevis belongs to the order P1euronectiformes, suborder Soleoidei, family Cynoglossidae, genus Cynoglossus, subgenus Arelia, and is a large bottom-floor flounder in warm temperate shallow seas. Flounder is the most widely distributed in the Yellow and Bohai Seas, but less in the East China Sea and the South China Sea. It is a marine flounder with important farming prospects. [0003] Zhou Liqing et al. (Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Journal of Fisheries, 2005, 29: 417-419) studied the karyotype of tongue sole half smooth, and the results showed that the number of diploid chromosomes ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 廖小林陈松林徐亘博徐营
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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