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Method for preparing capillary inner wall coating

A capillary and coating technology, which is applied in the field of preparation of capillary inner wall coating, can solve the problems of capillary blockage, complicated preparation, and many influencing factors, and achieves the effects of high reusability, simple preparation method and good coating stability.

Inactive Publication Date: 2010-12-15
邯郸开发区奥泰克生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The chemically bonded coated column has better stability, but the preparation is cumbersome and there are many influencing factors, and the reaction in the column can easily cause capillary clogging, which increases the cost of preparing the coated column to a certain extent, and the capillary column efficiency decreases after a period of use. Cannot be reused later
The dynamic adsorption coating can be replaced at any time, there is no life problem, but it is not easy to find the ideal coating material

Method used

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  • Method for preparing capillary inner wall coating
  • Method for preparing capillary inner wall coating
  • Method for preparing capillary inner wall coating

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0016] Example 1: Take an unused quartz capillary with an inner diameter of 50um, an outer diameter of 360um, and a length of 70cm. The capillary was cleaned with 50 mM citric acid solution for 2 minutes; then, a 3% polyethylene glycol 2000 coating buffer solution was slowly injected into the aforementioned capillary; until three drops flowed out from the other end of the capillary, the preparation of the coating was completed. The test effect will be better if the coated capillary filled with coating buffer is left for 12 hours. Carry out the experimental test of electrophoresis separation lactoferrin below:

[0017] (1) Experimental conditions:

[0018] Materials: the prepared capillary of Example 1, three samples of lactoferrin;

[0019] Electrophoresis operating conditions: PH value: 2.2; voltage: 16KV; current: 12uA; wavelength: 214nm

[0020] (2) Data processing table obtained from the experiment

[0021] Injection sequence number

example 2

[0022] Example 2: Take an unused quartz capillary with an inner diameter of 50um, an outer diameter of 360um, and a length of 70cm. The effective length is 60cm (the length from the injection port to the detection window). Use a buffer solution with a pH of 2.2 and a concentration of Wash the capillary with 50mM citric acid solution for 2 minutes; take a few grams of polyethylene glycol 2000, dissolve it in 50mM citric acid solution at pH=2.2, and make a coating buffer solution with a concentration of polyethylene glycol 2000 of 3%. ; Dissolve the lactoferrin sample to be detected in the coating buffer; slowly inject the coating buffer containing the lactoferrin sample into the capillary; put the capillary filled with the protein sample and the coating buffer at a voltage of 16KV, Current: 12uA, wavelength: 214nm electrophoresis operating conditions, while completing the separation of protein samples, the preparation of the coating is completed. Three samples were taken and th...

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Abstract

The invention discloses a method for preparing a capillary inner wall coating, and aims to provide the method for preparing the capillary inner wall coating which has the characteristics of quick, simple and convenient operation, low material cost and stable product performance. The method comprises the following steps of: rinsing a quartz capillary with the solution of citric acid; dissolving polyethylene glycol 2000 into the solution of the citric acid which has the pH of 2.2 and the concentration of 50mM and serves as a medium to prepare 0.8 to 10 percent coating buffer solution; dissolving a protein to be detected into the coating buffer solution; and performing electrophoretic separation on the protein, simultaneously finishing preparing the capillary inner wall coating. The method is suitable for the technical field of protein electrophoretic separation analysis.

Description

technical field [0001] The invention relates to a method for preventing protein adsorption in liquid phase separation technology, in particular to a preparation method for capillary inner wall coating in capillary electrophoresis technology. Background technique [0002] Capillary electrophoresis technology, also known as high-efficiency capillary electrophoresis, is a new type of liquid phase separation that uses capillary as the separation channel and high-voltage direct current electric field as the driving force to separate and analyze the components according to the differences in mobility and partition coefficient. technology. It is the development of gel electrophoresis technology and is a supplement to high performance liquid chromatography analysis. However, in practice, biological macromolecules such as proteins and nucleic acids, especially positively charged basic proteins, are easily adsorbed on the inner wall of the quartz capillary during electrophoresis, res...

Claims

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Application Information

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IPC IPC(8): G01N1/40G01N27/447C25D13/06
Inventor 陈农
Owner 邯郸开发区奥泰克生物科技有限公司
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