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Purification technology of rokitamycin

A karimycin and process technology, applied in the field of purification process for extracting karimycin, can solve the problem of less research on component purification, and achieve the effects of simple operation, improved quality and yield

Inactive Publication Date: 2010-12-22
EAST CHINA UNIV OF SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still few studies on removing impurities and optimizing components, especially as a new class of drugs, carrimycin has fewer reports on the purification of its components

Method used

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  • Purification technology of rokitamycin
  • Purification technology of rokitamycin
  • Purification technology of rokitamycin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Add 150 g of polyaluminum chloride to 25 L of carrimycin fermentation broth (titer 796u / ml), filter with plate and frame, and obtain 37.5 L of filtrate. Add 1.46 L of butyl acetate to the filtrate, while stirring, add 6 mol / L NaOH solution to adjust the pH to 8.5, continue to stir for 30 min and then let stand, add NaCl and separate to obtain 1.13 L of ester phase extract. Sequentially use 280mL water, NaH with a mass concentration of 1% 2 PO 4 solution and water to wash the butyl ester extract. Add 310 mL of pure water to the butyl ester extract with a volume of 1.07 L after washing, add 3 mol / L hydrochloric acid solution to adjust the pH to 2.1 while stirring, continue stirring for 30 min, then stand still and separate layers to obtain 325 ml of aqueous phase extract. Add 2mol / L NaOH solution to adjust the pH to neutral, pass N 2 Blow for 5h. Add 2mol / L NaOH solution to the obtained 300ml aqueous phase extract to adjust the pH to 10.0, let stand, filter with sucti...

Embodiment 2

[0033] Add 125g of polyaluminum chloride to 25L of carrimycin fermentation broth (titer 1470u / ml), and filter with a plate frame to obtain 40L of filtrate. Add 3.5 L of butyl acetate to the filtrate, while stirring, add 6 mol / L NaOH solution to adjust the pH to 8.2, continue stirring for 30 min, then let stand, and obtain 2.98 L of ester phase extract by layering. Wash the ester phase extract once with 745ml water, then use 745ml mass concentration of 0.7% NaH 2 PO 4 The solution washes the ester phase extract twice. Adding mass concentration is 0.7% NaH in the extraction solution that volume is 2.86L after washing 2 PO 4 Solution 1L, while stirring, add 3mol / L hydrochloric acid solution to adjust the pH to 2.2, continue to stir for 30min, then stand still and separate layers to obtain 1.04L aqueous phase extract. Add 2mol / L NaOH solution to adjust the pH to neutral, pass N 2 Blow for 5h. Add 2mol / L NaOH solution to the obtained 1.18L extract to adjust the pH to 9.8, let...

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Abstract

The invention discloses a purification technology for extracting rokitamycin from fermentation liquor, comprising the following steps: (1) adding a flocculating agent in the fermentation liquor to precipitate protein, and filtering to obtain a filtrate; (2) extracting the rokitamycin from the fermented filtrate obtained from step (1) by a solvent extraction method, and obtaining an ester phase; (3) alternatively washing the ester phase obtained from step (2) with a salt solution and pure water; (4) reextracting the rokitamycin from the ester phase to a water phase by a reextraction method; and (5) crystallizing and drying the water phase rokitamycin obtained from step (4) to obtain a product. Through extraction, especially through using a buffer salt solution to wash the extraction phase, the purification method removes residual spiramycin therein and optimizes the component proportion of the rokitamycin, the obtained product meets the quality index requirements, and the spiramycin in a washing liquid can be separated and recycled, thereby greatly improving the quality and the yield of the product.

Description

【Technical field】 [0001] The invention relates to the technical field of medicine and chemical industry, and more specifically, relates to a purification process for extracting carrimycin from fermentation liquid. 【Background technique】 [0002] Carrimycin, also known as Bitespiramycin, is the first new class of antibiotics in China. It is fermented by transgenic spiramycin-producing bacteria. It is a mixture of various structurally similar components formed by adding isovaleryl side chains to the 4″ position of spiramycin. The main component of the finished product is isovaleryl spiramycin I, II, III, in addition to a small amount of butyryl, propionyl, acetylspiramycin II, III and other acylated spiramycins, the chemical structure is shown in the figure below, and the specific components are shown in Table 1. The components of the finished product are required to be different The content of valerylspiramycin III is not less than 30%, the content of total isovalerylspiramy...

Claims

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Application Information

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IPC IPC(8): C07H17/08C07H1/06
Inventor 陈葵朱家文武斌纪利俊吴艳阳顾芨芨游旭郝玉有张海平
Owner EAST CHINA UNIV OF SCI & TECH
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