Mechanical hybrid rice seed production method utilizing transgenic technology of chloroplasts

A technology of hybrid rice and chloroplast, applied in the direction of plant gene improvement, recombinant DNA technology, botanical equipment and methods, etc., can solve the problems of complicated procedures and inability to produce seeds in large-scale farms, so as to reduce production costs and operation procedures of seed production , the effect of reducing labor intensity

Inactive Publication Date: 2010-12-29
HUNAN WEST CITY HYBRID RICE GENE TECH
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AI Technical Summary

Problems solved by technology

The process is complicated, and the entire seed production process requires sufficient labor to ensure that it is only suitable for popularization and application in labor-intensive rural areas. Large-scale farms cannot produce seeds due to lack of sufficient labor.

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0009] experiment procedure:

[0010] 1 Construction of green fluorescent protein gene carrier for chloroplast transformation

[0011] According to the published related sequences, primers were designed respectively, and a series of elements needed to construct rice plastid site-directed integration expression vectors were cloned by PCR technology: plastid ribosomal (16S) RNA operator promoter (Prrn), plastid psbA gene 3' end terminator (psbA3'), aminoglycoside 3'-adenylyltransferase gene (aadA), rice plastid genome high-frequency homologous recombination fragment (psbC / trnG, size 3362bp), and green fluorescent protein gene ( gfp). The rice plastid site-specific integration expression vector (-psbC-Prrn-gfp-aadA-psbA3'-trnG-) was constructed.

[0012] 2 Genetic transformation of rice chloroplasts

[0013] 2.1 Plant material selection

[0014] Rice (Oryza sativa L.) varieties used for genetic transformation of chloroplasts were selected from easily transformed hybrid rice b...

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PUM

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Abstract

The invention discloses a mechanical hybrid rice seed production method utilizing the transgenic technology of chloroplasts. The method is characterized by transforming the luminescent protein genes of the rice into the chloroplasts of restorer lines of the hybrid rice, wherein the restorer lines have the characteristic of maternal inheritance of fluorescent proteins and the pollens do not carry transgenes, therefore, the hybrids mated by the restorer lines are non-transgenic, proportionally sowing the male and female parents in a mixed manner during hybrid rice seed production, after becoming mature through field regular management, mechanically reaping the seeds and sorting the reaped seeds into non-fluorescing hybrids and fluorescing restorer lines through photoelectric sorting. The mechanical seed production technology not only greatly lightens the labor intensity of the farmers, reduces the operation procedures and lowers the production cost of the seeds but also ensures that the non-transgenic hybrid rice seeds are produced by utilizing the transgenic technology of chloroplasts.

Description

technical field [0001] The invention relates to a mechanized hybrid rice seed production method utilizing chloroplast transgene technology. Background technique [0002] my country's current hybrid rice seed production technology relies entirely on manual operations from sowing to harvesting. The parents are planted in stages, mostly the male parent is planted first, and the female parent is planted later; the female parent has multiple rows and the male parent has two rows. The process is complicated, and the entire seed production process requires sufficient labor to ensure that it is only suitable for popularization and application in labor-intensive rural areas. Large-scale farms cannot produce seeds due to lack of sufficient labor. Moreover, due to the factor of the male parent, the yield of seed production is reduced. The present invention will fundamentally change the current situation, realize mechanization without relying on labor force, and greatly increase the y...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82A01H1/02
Inventor 曹孟良邢俊杰唐丽袁隆平廖翠猛李丁谢灵灵陶小平赵迎曦
Owner HUNAN WEST CITY HYBRID RICE GENE TECH
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