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Detection of curative effect of antihypertensive drug

An antihypertensive and drug technology, applied in the fields of molecular biology and medicine, can solve problems such as difficulty in obtaining optimal blood drug concentration, toxic and side effects, and inability to effectively exert the curative effect of drugs

Inactive Publication Date: 2010-12-29
XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the genetic polymorphism of the CYP2D6 gene, there are differences in CYP2D6 enzyme activity among individuals, and polymorphisms in drug metabolism ability appear, which makes it difficult for patients to obtain the best blood drug concentration when taking medicine, such as blood drug concentration is too low to effectively exert the drug Toxic and side effects due to curative effect or high concentration

Method used

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Embodiment Construction

[0023] Below in conjunction with specific embodiment, further illustrate the present invention. For the experimental methods not indicated in the following examples, the specific conditions are usually in accordance with conventional conditions, or in accordance with the conditions suggested by the manufacturer.

[0024] The use of embodiment detection kit

[0025] Step 1: Extraction of DNA template

[0026] Genomic DNA of oral epithelial cells was extracted by silica gel adsorption.

[0027] Step 2: Real-time quantitative PCR reaction

[0028] Use the fluorescent quantitative PCR kit in the detection kit to perform 4 independent fluorescent quantitative PCR reactions. The total volume of each reaction is 10 μl, including 2 μl of DNA template with a concentration of 20 ng / μl, 1 μl of 10X fluorescent quantitative PCR reaction buffer , 0.1μl 25mM dNTP mixture, 0.6μl 25mM MgCl 2 solution, 0.025 μl (5 units / μl) Taq DNA polymerase, 0.225 μl each of 20 μM sense primer and antise...

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Abstract

The invention discloses a kit for detecting a curative effect of an individual antihypertensive drug. The kit comprises specific primer pairs of targeted locus CYP3A4 gene, CYP2D6 gene, CYP2C9 gene and CYP2C19 gene polymorphic locus for detecting the antihypertensive drug, specific fluorescent probe pairs, a fluorescent quantitive PCR conventional assembly and the like. The kit of the invention evaluates the curative effect of the individual antihypertensive drug by using the targeted locus CYP3A4 gene, CYP2D6 gene, CYP2C9 gene and CYP2C19 gene polymorphic locus for detecting the antihypertensive drug.

Description

technical field [0001] The present invention relates to the fields of molecular biology and medicine. More specifically, the present invention relates to a kit for detecting the curative effect of individual antihypertensive drugs. Gene polymorphism sites to assess the efficacy of individual antihypertensive drugs. Background technique [0002] Hypertension can be divided into primary and secondary two categories. In the vast majority of patients, the cause of hypertension is unknown, which is called essential hypertension, accounting for more than 95% of the total hypertension; in less than 5% of patients, elevated blood pressure is a clinical manifestation of certain diseases Performance, itself has a clear and independent cause of disease, known as secondary hypertension. [0003] Primary hypertension is a syndrome in which elevated blood pressure is the main clinical manifestation, referred to as hypertension. Hypertension is an important cause and risk factor of vari...

Claims

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Application Information

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IPC IPC(8): C12Q1/68G01N21/64
Inventor 冯哲民邹祖烨
Owner XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
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